A cost-effective, straightforward and modifiable 2.5D microfabrication methodology, as we term multi-layertape lithography, is presented here for the first time. It uses a commercial scalpel to prototype 2.5D multilevel microchannels on commercial tape as thin as 500µm in 10 minutes. Three functional microfluidic devices are applied with this methodology, and display high performance regarding microdroplet formation, multiphase flux and self-powered sequential fluid delivery. We find the microchannel height of a 2.5D 15 microchip can efficiently control capillary force-driven flow velocity. The autonomous sample flow rates through 55 mm long microchannels are 0.1 µL/s, 0.21 µL/s and 0.39 µL/s when multilevel microchannel heights are 200 µm, 300 µm, and 400 µm, respectively.20After detachment of two, one, and one layers of tape from the three microchannels of a 2.5D tape-master, the microchannel heights are modified to 100 µm, 250 µm and 300 µm, with the autonomous sample flow rate changing to 0.03 µL/s, 0.15 µL/s and 0.28 µL/s, 25 correspondingly. In contrast with 2D microfabrication technology, we anticipate that multi-layer tape lithography will pave the way for researchers, especially those from resource-limited labs, to develop costeffective, practical, self-powered, and disposable 2.5D 30 microfluidic devices.