A microelectrode-based sensor for label-free in vitro detection of ischemic effects on cardiomyocytes

Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

“…If distinct morphological alterations were the main cause, cell membrane capacitance and resistance would be changed significantly as shown in previous studies Krinke et al, 2009). Based on a simplified equivalent circuit (Fig.…”

“…In contrast, label-free bioelectronic monitoring techniques based on e.g. electrochemical impedance spectroscopy are suitable for sensitive measurements of cellular alterations (Jahnke et al, 2012;Kloss et al, 2008;Krinke et al, 2009;Poenick et al, 2014). More strikingly, in a previous work we were able to demonstrate the impedance spectroscopy based indirect detection of Y1-receptor activation (Wolf et al, 2008).…”

A novel 96-well multielectrode array based impedimetric monitoring platform for comparative drug efficacy analysis on 2D and 3D brain tumor cultures

“…In research, 2D cell culture systems are widely used to investigate cellular response to drug treatment Krinke et al, 2009;Liu et al, 2009). Since 2D cultures have shown to be an expedient but still highly artificial representation of the in vivo situation (Hirschhaeuser et al, 2010), 3D cultures have been introduced to mimic the corresponding in vivo tissues and allow studying of basic mechanisms like cell growth, proliferation and differentiation in a reproducible format (Kunz-Schughart et al, 1998) as well as for testing novel treatment strategies (Hirschhaeuser et al, 2010).…”

Direct Chemosensitivity Monitoring Ex Vivo on Undissociated Melanoma Tumor Tissue by Impedance Spectroscopy

“…Although molecular biologic end-point assays on 3D cultures are in general more laborious and/or can be easily falsified when the readout (e.g., absorbance or fluorescence) is performed directly on the 3D culture, label-free bioelectronic methods have recent advantages. Especially, impedance spectroscopy is a suitable technique for characterization of single tumor cells (14) as well as cellular degradation in 2D cultures (15,16) and, more strikingly, for quantitative monitoring of chemotherapeutic efficacy in 3D cultures (17,18). Using our unique microcavity array (MCA) technology, we could detect the sensitivity or even an aggressive response to certain chemotherapeutics within 3D in vitro tumor models (19).…”