Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Kamp, Verena te; Lindner, Ricco; Jahnke, Heinz‐Georg; Krinke, Dana; Kostelnik, Katja B.; Beck‐Sickinger, Annette G.; Robitzki, Andrea A.

doi:10.1016/j.bios.2014.08.066

“…Afterwards, this effect diminished for concentrations up to 30 nM A2M*. Cellular alterations that could reflect this impedimetric effect course could be among others an increased cell-cell and/or cell substrate adhesion [ 14 ]. This correlates with the increased adhesion of tumour cells upon A2M* treatment ( Fig 1A ) .…”

Section: Resultsmentioning

confidence: 99%

The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat

Kurz

¹

,

Thieme

²

,

Amberg

³

et al. 2017

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Cancer resistance is a major cause for longevity of the naked mole-rat. Recent liver transcriptome analysis in this animal compared to wild-derived mice revealed higher expression of alpha2-macroglobulin (A2M) and cell adhesion molecules, which contribute to the naked mole-rat’s cancer resistance. Notably, A2M is known to dramatically decrease with age in humans. We hypothesize that this might facilitate tumour development. Here we found that A2M modulates tumour cell adhesion, migration and growth by inhibition of tumour promoting signalling pathways, e.g. PI3K / AKT, SMAD and up-regulated PTEN via down-regulation of miR-21, in vitro and in tumour xenografts. A2M increases the expression of CD29 and CD44 but did not evoke EMT. Transcriptome analysis of A2M-treated tumour cells, xenografts and mouse liver demonstrated a multifaceted regulation of tumour promoting signalling pathways indicating a less tumorigenic environment mediated by A2M. By virtue of these multiple actions the naturally occurring A2M has strong potential as a novel therapeutic agent.

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“…Herein, we demonstrate that also the larger CD with a DP of 10 to 12 and a mixture of CD8–CD15 were not toxic to the investigated human cell lines. Sensitive techniques such as multielectrode array‐based impedimetric monitoring allowed the detection of even small changes in cellular adhesion and cell contacts . We used interdigital electrode arrays in combination with the cell lines for the sensitive impedimetric detection of cellular alterations caused by the CD .…”

Section: Figurementioning

confidence: 99%

“…Sensitive techniques such as multielectrode array‐based impedimetric monitoring allowed the detection of even small changes in cellular adhesion and cell contacts . We used interdigital electrode arrays in combination with the cell lines for the sensitive impedimetric detection of cellular alterations caused by the CD . The results of impedimetric analyses obtained with CD10 are shown in Figure b and for CD11 and CD12 shown in Figure S8 in the Supporting Information.…”

Section: Figurementioning

confidence: 99%

Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals

Sonnendecker

¹

,

Thurmann²,

Przybylski

³

et al. 2019

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Large-ring cyclodextrins (CD) are cyclic glucans composed of 9o rm ore a-1,4-linked glucose units.T hey are minor side products of bacterial glucanotransferases (CGTases,E C2.4.1.19) and have previously been available only in very small amounts for studies of their properties in supramolecular complex formation reactions.W ee ngineered aCGTase to synthesizemainly large-ring CD facilitating their preparation in larger amounts.B yr eversed phase chromatography,w eo btained single CD samples composed of 10 to 12 glucose units (CD10, CD11, and CD12) with ap urity of > 90 %. Their identity was confirmed by high resolution mass spectrometry and fragmentation analysis.Wedemonstrated the non-toxicity of CD10-CD12 for human cell lines by ac ell proliferation assaya nd impedimetric monitoring.W et hen showed that CD10 and CD11 are efficient chiral selectors for the capillary electrophoretic separation of the enantiomeric pharmaceuticals fluvastatin, mefloquine,c arvedilol, and primaquine.CDa re a-1,4-linked cyclic glucans with ad egree of polymerization (DP) between 6t o> 100. Branched a-1,6-CD have also been described. [1] In contrast to larger CD,CD6, CD7, and CD8 (a-, b-, g-CD) have been studied extensively and are produced commercially. [2] They form cone-shaped structures with ahydrophobic cavity and an outer hydrophilic area, enabling the reversible binding of hydrophobic mole-Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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“…[21] Herein, we demonstrate that also the larger CD with aDPof10to 12 and amixture of CD8-CD15 were not toxic to the investigated human cell lines.Sensitive techniques such as multielectrode array-based impedimetric monitoring allowed the detection of even small changes in cellular adhesion and cell contacts. [22,23] We used interdigital electrode arrays in combination with the cell lines for the sensitive impedimetric detection of cellular alterations caused by the CD. [22][23][24] Ther esults of impedimetric analyses obtained with CD10 are shown in Figure 2b and for CD11 and CD12 shown in Figure S8 in the Supporting Information.…”

mentioning

confidence: 99%

“…[22,23] We used interdigital electrode arrays in combination with the cell lines for the sensitive impedimetric detection of cellular alterations caused by the CD. [22][23][24] Ther esults of impedimetric analyses obtained with CD10 are shown in Figure 2b and for CD11 and CD12 shown in Figure S8 in the Supporting Information. Hemolytic effects of CD6-CD9 on human erythrocytes have previously been studied.…”

mentioning

confidence: 99%

Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals

Sonnendecker

¹

,

Thurmann²,

Przybylski

³

et al. 2019

Self Cite

View full text Add to dashboard Cite

Large-ring cyclodextrins (CD) are cyclic glucans composed of 9o rm ore a-1,4-linked glucose units.T hey are minor side products of bacterial glucanotransferases (CGTases,E C2.4.1.19) and have previously been available only in very small amounts for studies of their properties in supramolecular complex formation reactions.W ee ngineered aCGTase to synthesizemainly large-ring CD facilitating their preparation in larger amounts.B yr eversed phase chromatography,w eo btained single CD samples composed of 10 to 12 glucose units (CD10, CD11, and CD12) with ap urity of > 90 %. Their identity was confirmed by high resolution mass spectrometry and fragmentation analysis.Wedemonstrated the non-toxicity of CD10-CD12 for human cell lines by ac ell proliferation assaya nd impedimetric monitoring.W et hen showed that CD10 and CD11 are efficient chiral selectors for the capillary electrophoretic separation of the enantiomeric pharmaceuticals fluvastatin, mefloquine,c arvedilol, and primaquine.CDa re a-1,4-linked cyclic glucans with ad egree of polymerization (DP) between 6t o> 100. Branched a-1,6-CD have also been described. [1] In contrast to larger CD,CD6, CD7, and CD8 (a-, b-, g-CD) have been studied extensively and are produced commercially. [2] They form cone-shaped structures with ahydrophobic cavity and an outer hydrophilic area, enabling the reversible binding of hydrophobic mole-Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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Quantitative impedimetric NPY-receptor activation monitoring and signal pathway profiling in living cells

Cited by 9 publications

References 29 publications

The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat

The anti-tumorigenic activity of A2M—A lesson from the naked mole-rat

Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals

Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals

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