A Microassay for Nitroblue Tetrazolium Reduction by Human Neutrophils

Hofstaetter, T.; Brammsen, H.

doi:10.1016/s0171-2985(81)80086-1

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…Under the experimental conditions chosen neither St-IgG, S-IgG, nor R-IgG stimulated the oxi dative metabolism of PMN as measured by the intracellular reduction of the dye, nitroblue tétrazolium (NBT) [6], irrespective of whether autologous serum as complement source was present or not (not shown). ICs (10 pg/ml) consisting of tetanus toxoid (TT) and anti-TT standard IgG gave a 2.5-fold stimulation of PMN over control values ( fig.…”

Section: Activation Of Phagocyte Oxidative Burstmentioning

confidence: 96%

“…Particle binding and ingestion induces an oxidative burst in phagocytes, which has some correlation with their bactericidal ca pacity [6,11]. This response to the different ICs tested here was measured for human PMN and monocytes by separate tech niques.…”

Section: Activation Of Phagocyte Oxidative Burstmentioning

confidence: 96%

“…Human polymorphonuclear granulocytes (PMN) were isolated from the peripheral blood of healthy vol unteers by centrifugation through a two-step FicollHypaque (Seromed, Munich, FRG) gradient according to the method of English and Anderson as described [6], Monocytes were obtained from the mononuclear cell fraction by adherence to plastic Petri dishes for 60 min at 37 "C. They were used in the assays after 4-10 days culture in serum-free medium. At least 98% of these cells were esterase-positive.…”

Section: Cellsmentioning

confidence: 99%

“…Nitroblue tétrazolium (NBT) reduction by human PMN was measured by a microassay as described pre viously [6]. Briefly, 2xl05 PMN were incubated with lOpg IC in 0.2ml RPMI 1640 with or without autolo gous serum for 45 min at 37 "C. Each sample was assayed eight-fold.…”

Section: Stimulation Of Phagocyte Oxidative Metabolismmentioning

confidence: 99%

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S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

Hofstaetter¹,

Gronski²,

Kanzy³

et al. 1983

Vox Sanguinis

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The generation and release of mediators of inflammation and anaphylaxis via activation of complement or of Fc receptor-bearing cells is held responsible for adverse reactions observed upon intravenous administration of standard immunoglobulins. They are caused by immunoglobulin G (IgG) effector functions predominantly located in the Fc region of the molecule and ‘activated’ by aggregate formation. Their functional activity depends on the correct conformation of the Cy2 domains of Fc and are therefore impaired or even abrogated by S-sulfonation of the hinge disulfide bonds, as demonstrated in this communication: S-sulfonated IgG (S-IgG) has no anticomplementary activity and does not interact with Fc-receptors anymore. After antigen binding, i. e. immune complex (IC) formation, sulfonated IgG is about half as potent as standard IgG in complement activation or phagocyte stimulation (human monocytes and granulocytes). The two activities synergize, however, so that in the presence of complement S-IgG IC are as effective phagocyte activators as standard IC. Moreover, S-sulfonation being the only chemical modification of imunoglobulins that is reversible, it can be demonstrated that all IgG effector functions important for antigen removal are regained by reconstitution of the disulfide bonds.

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Section: Activation Of Phagocyte Oxidative Burstmentioning

confidence: 96%

Section: Activation Of Phagocyte Oxidative Burstmentioning

confidence: 96%

Section: Cellsmentioning

confidence: 99%

Section: Stimulation Of Phagocyte Oxidative Metabolismmentioning

confidence: 99%

See 2 more Smart Citations

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

Hofstaetter¹,

Gronski²,

Kanzy³

et al. 1983

Vox Sanguinis

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In vitro chemosensitivity testing of leukemic cells: Development of a semiautomated colorimetric assay

Bernabei

Santini

Silvestro

et al. 1989

Hematological Oncology

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A rapid chemosensitivity assay was developed, employing the human continuous leukemic cell lines HL 60, K 562, FLG 29.1. This automated colorimetric assay is based on the characteristic of viable, metabolically active cells to cleave p-iodonitrotetrazolium violet (INT) into a red formazan derivative, whose optical density is readable at 492 nm by an automated microtiter-plate reader photometer. A linear relationship was found between the viable cell number and the optical density of INT cleaved by the cellular samples. Dead cells did not reduce INT and did not interfere with the formazan derivative generation and the photometric reading. Leukemic cell lines were also tested for INT formazan derivative generation after exposure to antileukemic drugs at various concentrations, representative of plasma levels obtainable in vivo. A dose-dependent inhibition was detected, with different sensitivity patterns, related both to the drugs and to the different cell lines. A significant correlation between the viable cell number and the amount of tetrazolium salt cleaved was also demonstrated after drug exposure. INT assay allows the processing of a great number of samples and gives the opportunity to screen several drugs, saving time and yielding fully reliable results.

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Immunostimulation as a Therapeutic Principle in Bacterial Infections: The Effect of the Immunomodulator Bestatin on the Experimental Chronic E. coli Urinary Tract Infection

Dickneite

Schorlemmer²,

Hofstaetter³

et al. 1985

Experimentelle Urologie

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A Microassay for Nitroblue Tetrazolium Reduction by Human Neutrophils

Cited by 7 publications

References 18 publications

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

In vitro chemosensitivity testing of leukemic cells: Development of a semiautomated colorimetric assay

Immunostimulation as a Therapeutic Principle in Bacterial Infections: The Effect of the Immunomodulator Bestatin on the Experimental Chronic E. coli Urinary Tract Infection

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