A microarray for microRNA profiling in mouse testis tissues

Abstract

MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles by repressing translation or cleaving RNA transcripts. Recent studies indicate that miRNAs are mechanistically involved in the development of mammalian spermatogenesis. However, little work has been done to compare the miRNA expression patterns between immature and mature mouse testes. Here, we employed a miRNA microarray to detect 892 miRNAs in order to evaluate the expression patterns of miRNA. The expression of 19 miRNAs was significantly different between immature and mature individuals. Fourteen miRNAs were significantly upregulated and five miRNAs were downregulated in immature mice and this result was further confirmed by a quantitative real-time RT-PCR assay. Many target genes involved in spermatogenesis are predicted by MiRscan performing miRNA target scanning. Our data indicated specific miRNAs expression in immature mouse testis and suggested that miRNAs have a role in regulating spermatogenesis.

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

“…However, the effect of TBHP-induced oxidative stress on miRNA expression has not been investigated in mouse testis so far. Based on microarray information of miRNAs expression profile in mouse testis [57], five miRNAs were selected to examine this idea whether failure in spermatogenesis associated with oxidative stress is at least partly due to changes in testicular miRNAs profile.…”

“…To achieve this goal, five miRNAs (miR-181b, miR-34a, miR-449, miR-122a and let-7e) were selected to examine their expression levels, according to the study of Yan et al [57]. The selected miRNAs are involved in cellular processes such as cell cycle control and apoptosis.…”

A multi-faceted approach to understanding male infertility: gene mutations, molecular defects and assisted reproductive techniques (ART)

“…In a recent study, high levels of expression of specific miRNAs was observed in low fertility bulls, which could suggest that miRNAs might be responsible for down regulating gene expression and thereby, regulate proteins that have important roles in fertilization and early embryonic development [187]. Other studies revealed significant differences in the expression of miRNAs between immature and mature individuals, suggesting miRNAs have a role in regulating spermatogenesis [188,189]. Special mention needs to be made of miR-322, miR-323, miR-372 and miR-373 that are presumed to be novel oncogenes that participate in the development of human testicular germ cell tumors [190].…”

Specific microRNA expression during chondrogenesis of human mesenchymal stem cells

“…Procedures were performed as described previously (23,24). Briefly, low-molecular-weight RNAs were enriched from 100 μg total RNA [sample A, B, C (25)] with mirVana™ miRNA isolation kit (Ambion) and amplified with the NCode™ miRNA amplification system (Invitrogen).…”