Specific microRNA expression during chondrogenesis of human mesenchymal stem cells

Abstract

Abstract. Mesenchymal stem cells (MSCs) have the capacity to self-renew and differentiate into multiple cell types, but little is known about the precise mechanism of this procedure. Recent studies show that a group of short noncoding RNAs called microRNAs (miRNAs) play a key role in this procedure. However, little work has been done to determine the miRNAs which specifically regulate the differentiation of MSCs. In this study, we cultured human MSCs and chondrogenic differentiation MSCs of 3 donors, and investigated the miRNA expression profiles of MSCs and chondrogenic differentiated MSCs from 2 donors by using miRNA microarrays. We found 5 miRNAs were significantly overexpressed in chondrogenic differentiated MSCs in each sample, and 4 were further confirmed by real-time RT-PCR assay in the sample from the third independent donor. We also predicted the confirmed miRNAs corresponding to putative target genes by online software. The results indicated that the overexpressed miRNAs in chondrogenic differentiated MSCs may play a role in the procedure of MSC chondrogenesis, which offers us guidance for further studies.

MicroRNA‐193b‐3p regulates matrix metalloproteinase 19 expression in interleukin‐1β‐induced human chondrocytes

“…Simultaneously, the iNOS gene suppression by miR‐193b‐3p was detected in this study; however, neither TNF‐α nor iNOS was proven to precisely regulate—miR‐193b‐3p as its target. Recent studies have reported that miR‐193b‐3p was overexpressed during chondrogenesis of human adipose‐derived stem cells and human bone marrow‐derived mesenchymal stem cells, and regulated early chondrogenic differentiation with the help of TGFBR2 and TGFBR3 . Yet, the TGF‐β signaling pathway, regulated by miR‐193b‐3p, appears to play a more essential role in both chondrocyte differentiation and osteoarthritis development and progression.…”

Epigenetic and Proteomic Signatures for Chronic Pain Patients after Total Knee Replacement

“…In regards to hsa-miR-130b-3p, there is still a lack of evidence concerning the action of it in relation to pain conditions, but established is its involvement at different levels in the pathophysiology of several types of tumors 94,282 . Moreover, the dysregulation of this miRNA has been associated to several pathological conditions such as obesity, or resistance to insulin, but has been also found involved in physiological process which includes osteo and chondrogenesis 101,169,171 . In Study II the results find hsa-miR-130b-3p positively dysregulated in the preoperative circulation of KOA patients with bad postoperative recovery, but more evidence is necessary to validate the involvement of this miRNA for post-operative pain conditions in patients with KOA and show whether it can serve as a potential biomarker (Study II).…”