A metrological approach for the assessment of selenium status in human serum

Atomic spectrometry update. Elemental speciation review

“…These materials, which have certied and indicative values for total Se respectively, were examined by SSIDMS. 94 The SeMet was extracted from 0.45 g serum, aer the addition of a 76 SeMet, with 6 mol L À1 urea and 0.1 mol L À1 dithiothreitol with mechanical shaking for 1 h. Following the addition of a mixture of protease (15 mg mL À1 ) and lipase (8 mg mL À1 ) the mixture was shaken for a further 15 min and incubated at 37 C for 17 h in order to complete the enzymatic reaction. Finally, 20 mL of glacial acetic acid was added and the resulting solution stored at À20 C until analysis.…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…The rationale for determination of measurement uncertainty, together with validation of an analytical method, so as to give high quality data which may be assessed with respect to statutory limits, reference limits and data from other studies, was presented by Bocca et al 18 In applying their principles to the validation of analytical procedures to measure 20 elements in human blood, these workers reported linearity, LODs, bias on CRMs, repeatability, reproducibility and expanded uncertainty. In keeping with this philosophy, Jitaru et al 19 measured six elements in tomato paste by HR-ICP-MS, presenting the method validation data including the measurement uncertainty.…”

[Sec-to-Cys]selenoprotein – a novel type of recombinant, full-length selenoprotein standard for quantitative proteomics

“…19 In contrast, selenium speciation, especially selenoprotein quantication, is more challenging since no appropriate protein standards are available so far. 20 Various approaches for selenoprotein quantication have been reported previously. They include SeAlb quantication at the level of SeMet released from SeAlb by enzymatic hydrolysis, 20,21 species-unspecic ID ICP MS 22,23 for SEPP1 and GPx3, as well as methods using antibodies 10 and on-line external calibration.…”

“…20 Various approaches for selenoprotein quantication have been reported previously. They include SeAlb quantication at the level of SeMet released from SeAlb by enzymatic hydrolysis, 20,21 species-unspecic ID ICP MS 22,23 for SEPP1 and GPx3, as well as methods using antibodies 10 and on-line external calibration. 24 The analytical approach combining online affinity liquid chromatography coupled to ICP MS, laser ablation ICP MS and ESI MS/MS has been presented recently.…”