A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation

Abstract

Three different dutasteride extraction methods were compared and a method based on liquid-liquid extraction (LLE) using methyl tert-butyl ether and methylene chloride was proved to be more effective than others for the extraction of dutasteride and finasteride, the internal standard (IS), from rat plasma. Additionally, a method composed of the LLE extraction, liquid chromatography, and multiple reaction monitoring (MRM) to target dutasteride and IS was validated by assessing specificity, linearity (r 2 = 0.9993, 5 -400 ng/mL), sensitivity (the limit of detection: 4.03 ng/mL; the limit of quantitation: 12.10 ng/mL), accuracy (intra-day: 89.4 -105.9%; inter-day: 84.9 -100.9%), precision (intra-day: 0.8 -6.9%; inter-day: 2.9 -15.9%), and recovery (84.7 -107.8%). Since the validated method was successfully applied to a pharmacokinetic study of dutasteride, it can be useful for the pharmacokinetic evaluation of newly developed dutasteride formulations.

Simultaneous screening of 21 potential adulterants in dietary supplements for the treatment of prostate diseases using ultra‐performance liquid chromatography and liquid chromatography‐electrospray ionization‐tandem mass spectrometry

“…Two to three product ions (Q3) were compared with various references. As a result, 19 drug compounds were found to be nearly identical to various references [21][22][23][40][41][42][43]. For two other compounds that either lacked references or did not match the references (emepronium and imidafenacin), their fragmentation patterns were monitored according to the collision energy obtained from LC-Q-TOF/MS analysis.…”

“…Previously, for the development of a more rapid and accurate monitoring method, drug compounds for a few prostate-related diseases or erectile dysfunction were analytical targets, and methods such as liquid chromatography and spectrometry using HPLC, HPTLC, LC-MS, and LC-ESI-MS/MS have been routinely used for analysis [16][17][18][19][20][21][22][23][24][25]. However, these methods have certain limitations such as the low number of detectable target compounds, or sample preparation could only be performed in a limited manner using matrices to exclude soft capsule type of dietary supplements, which contain oils as an ingredient [26][27][28][29].…”