A method to improve histopathological analysis of prostate biopsies: the “sandwich” technique

Minardi, Daniele; Galosi, Andrea Benedetto; Dell’Atti, Lucio; Yehia, Mohamed; Montironi, Rodolfo; Polito, Massimo; Muzzonigro, Giovanni

doi:10.1016/s1569-9056(02)80457-2

Cited by 3 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore we reviewed our prostate biopsy database composed by 4,500 cases, most of them using the pre-embedding technique plus inking the peripheral end of PB. We described our technique in previous reports [ 11 , 12 ]. Therefore, results are based on literature search and our experience.…”

Section: Methodsmentioning

confidence: 99%

Specimen Orientation by Marking the Peripheral End: (Potential) Clinical Advantages in Prostate Biopsy

et al. 2011

View full text Add to dashboard Cite

The aim of this paper is to identify advantages that could be obtained by orientation of the biopsy specimen using the marking technique. We reviewed our experience (4,500 cases) and the published literature. The peripheral (proximal) end of the fresh specimen is marked with ink soon after needle delivering in a few minutes. It is performed easily in association with pre-embedding method. Five potential clinical advantages were identified: (1) tumor localization, (2) atypical lesions localization and planning rebiopsy strategy, (3) planning surgical strategy, (4) selection criteria for focal therapy and active surveillance, and (5) cost reduction. Peripheral end marking is low cost, easy and reproducible. It drives several potential advantages in cancer diagnosis or isolated atypical lesions, in particular, spatial localization within the biopsy (transition versus peripheral zone, anterior versus posterior, subcapsular versus intraparenchima, and extraprostatic extension) should be easy and reliable. We can add a new pathological parameter: pathological orientation or biopsy polarity.

show abstract

Section: Methodsmentioning

confidence: 99%

Specimen Orientation by Marking the Peripheral End: (Potential) Clinical Advantages in Prostate Biopsy

et al. 2011

View full text Add to dashboard Cite

show abstract

“…After these publications, a second series of articles was published [10,19,20,21] with data about optimizing Rogatsch's preembedding method. These studies inked the proximal end of a core and used a modified ‘sandwich' technique (1 core between 2 sponges presoaked with fixative liquid).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we compared four preembedding methods: Rogatsch's method modified by colleagues from Ancona (‘sandwich' method) [10,11], the method of wrapping cores in a piece of paper (on the edge of biplicated paper) by Urbanskii et al [12] in our modification (filter paper was presoaked with normal saline), the method of putting cores in grooves on glass (preembedding glass) [13,14], and our new method described in this article in which cores were put on the edge of thick cardboard. The first two methods were chosen because most guidelines and reviews advise to use them or their modifications, whereas the two other methods have been described recently.…”

Section: Methodsmentioning

confidence: 99%

“…Afterwards the sponge was placed in a standard cassette for histological samples and covered with another presoaked sponge. Unlike the authors of the method [10,11], we did not ink the proximal end of the biopsy specimen.…”

Section: Methodsmentioning

confidence: 99%

“…They suggest sandwiching biopsy cores between two nylon meshes or enveloping them in a piece of paper. Reviews from another group recommend their ‘sandwich' technique of using sponges soaked with formalin or normal saline [9,10]. …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prostate Biopsy Core Handling: Comparison of Contemporary Preembedding Methods

Pryalukhin

Vandromme²,

Dellmann³

et al. 2015

Urol Int

View full text Add to dashboard Cite

Introduction: The probability of prostate cancer detection is related to the amount of tissue represented. For optimal tissue representation, the specimens should preserve their regular cylindrical shape and avoid artefacts and deformation caused by fixation and preembedding. The aim of our study was to compare contemporary preembedding methods including a new method of using thick cardboard. Materials and Methods: To compare the preembedding methods, we took 36 nonfragmented cores from fresh prostatectomy specimens for each method, fixed them in formalin and made histological slides. The comparison criteria were a core section area in the middle section and the number of fragments per core after processing. Results: Two methods (preembedding on the edge of thick cardboard and on biplicated paper) provided a bigger section area of specimens. The differences in amounts of fragments were very small among the methods mentioned above and the preembedding glass with grooves, but preembedding between two sponges in a histological cassette showed higher fragmentation. Conclusions: Preembedding on the edge of thick cardboard and on biplicated paper can be recommended as effective methods of prostate biopsy core fixation. Paper or cardboard for fixation of prostate biopsy cores should be presoaked with formalin or normal saline.

show abstract

Biomarker detection technologies and future directions

Nimse

Sonawane

Song³

et al. 2016

Analyst

202

137

View full text Add to dashboard Cite

Biomarkers play a vital role in disease detection and treatment follow-up. It is important to note that diseases in the early stage are typically treated with the greatest probability of success. However, due to various technical difficulties in current technologies for the detection of biomarkers, the potential of biomarkers is not explored completely. Therefore, the developments of technologies, which can enable the accurate detection of prostate cancer at an early stage with simple, experimental protocols are highly inevitable. This critical review evaluates the current methods and technologies used in the detection of biomarkers. The aim of this article is to provide a comprehensive review covering the advantages and disadvantages of the biomarker detection methods. Future directions for the development of technologies to achieve highly selective and sensitive detection of biomarkers for point-of-care applications are also commented on.

show abstract

A method to improve histopathological analysis of prostate biopsies: the “sandwich” technique

Cited by 3 publications

References 0 publications

Specimen Orientation by Marking the Peripheral End: (Potential) Clinical Advantages in Prostate Biopsy

Specimen Orientation by Marking the Peripheral End: (Potential) Clinical Advantages in Prostate Biopsy

Prostate Biopsy Core Handling: Comparison of Contemporary Preembedding Methods

Biomarker detection technologies and future directions

Contact Info

Product

Resources

About