A method to detect the parasitic nematodes from the family Anisakidae, in Sardina pilchardus, using specific primers of 18 S DNA gene

Real-time PCR assay for detection of Trichinella in meat

“…In the previous studies of molecular detection it was not tested, and it could lead to falseepositive assignment (Santos, Sasal, Verneau, & Lenfant, 2006). The present study reflect the specificity of primers used, confirming absence of amplification with meat of pig, wild board, deer and horse, which could be the more probably source of contamination for human (Boireau et al, 2000).…”

Detection of anisakids in fish and seafood products by real-time PCR

“…In the great majority of previous studies the specificity of primers used were not tested with other related parasites or host fish (Abe et al, 2006;Kijewska et al, 2002;Szostakowska et al, 2002), among them worth mentioning, the work of Santos et al (2006) which can lead to false positive assignment for unspecific or unexpected bands (Santos et al, 2006).On the contrary, in both our previous (Espiñeira et al, 2010) and current studies in which the specificity is checked with related parasites and numerous host fish and cephalopods belonging to different families and orders.…”

“…Different genetic techniques for the identification of Anisakis and other parasites have been developed in last years PCReRFLP (Abe, Tominaga, & et al, 2006;Espiñeira, Herrero, & et al, 2010;Kijewska, Rokicki, & et al, 2002;Szostakowska, Myjak, & et al, 2002), multiplex or specific PCR assay (Chen et al, 2008;Santos, Sasal, & et al, 2006;Umeharaa, Kawakamia, & et al, 2007) SSCP analysis (Zhang, Hu, & et al, 2007;Zhu et al, 2007) and ALF (Espiñeira et al, 2010).…”

Detection and identification of anisakids in seafood by fragment length polymorphism analysis and PCR–RFLP of ITS-1 region

“…In the great majority of previous studies the specificity of primers used not were tested with others related parasites or host fishes (Abe et al, 2006;Kijewska et al, 2002;Szostakowska et al, 2002), among them worth mentioning, the work of Santos et al (2006) which can lead to false positive assignment for unspecific or unexpected bands (Santos et al, 2006). In most of works the sensibility of the methods were not tested either, in some cases dilutions of DNA for each species are used for this task (Chen et al, 2008).…”

“…Kijewska et al developed a method to identify 11 species of parasites using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method (PCR-RFLP) (Kijewska, Rokicki, Sitko, & Wegrzyn, 2002). Also Santos et al designed a methodology to detect parasitic nematodes, but the primer sets designed were not specific for the Anisakidae family and the method was only applied to Sardina pilchardus (Santos et al, 2006).…”