Detection and identification of anisakids in seafood by fragment length polymorphism analysis and PCR–RFLP of ITS-1 region

Espiñeira, Montserrat; Herrero, Beatriz; Vieites, Juan M.; Santaclara, Francisco J.

doi:10.1016/j.foodcont.2009.12.026

Cited by 18 publications

(13 citation statements)

References 48 publications

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“…12 out of 16 endonucleases cut the target sequence in one or more of the species, while four endonucleases (DraI, EcoRV, PstI and SacI) did not (D'Amelio et al, 2000). Many studies have been performed on the ITS region with HinfI and HhaI for all species of Anisakid nematodes (Espiñeira et al, 2010;Shih et al, 2010). In this study, the restriction profiles of Anisakis spp.…”

Section: Discussionmentioning

confidence: 83%

Occurrence and prevalence of fish-borne Anisakis larvae in the spotted mackerel Scomber australasicus from Taiwanese waters

Chen

2015

Acta Tropica

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Section: Discussionmentioning

confidence: 83%

Occurrence and prevalence of fish-borne Anisakis larvae in the spotted mackerel Scomber australasicus from Taiwanese waters

Chen

2015

Acta Tropica

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“…These findings were the basis for using the PCR-RFLP based approach to detect yacon in real herbal tea mixtures, as is presented here. PCR-RFLP was reported by Espiñeira et al (2010) as an alternative to sequencing, and it has been widely used in many previous analyses to confirm the identity of a particular PCR product (Espiñeira et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Žiarovská¹,

Rajchl²,

Lojka³

et al. 2016

Czech J. Food Sci.

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AbstractŽiarovská J., Rajchl A., Fernández E., Prchalová J., Milella L. (2016): Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods. Czech J. Food Sci., 34: 495-502.The identification of yacon, a medicinal plant, in tea mixtures by rapid Polymerase Chain Reaction (PCR) and the Direct Analysis in Real Time coupled with Time-of-Flight Mass Spectrometry (DART/TOF-MS) method were evaluated. Three tea products and a pure yacon tea were analysed using the molecular method PCR, concretely the intraspecific variation of the internal transcribed spacer (ITS) regions of rDNA and the DART method coupled with TOF-MS. The results show the reliability of PCR and restriction cleavage of the ITS as a combined approach to confirm the presence of yacon in herbal tea mixtures. Three fragments of approximately 700, 408, and 235 bp in length are present when yacon is a part of the herbal tea mixture. The Principal Component Analysis (PCA) based on the fingerprints of the complete Total Ion Current (TIC) mass spectra shows sufficient separation of herbal teas with and without yacon leaves. The reported methods are technically rapid and can be used as an effective tool for the purposes of yacon identification or authentication.

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“…Beyond pathogen screening there is interest in screening meat products for authenticity and DNA from foods associated as a source of allergens, for example peanut (Espineira et al, 2010 andMeyer, 2000). There have been significant advances in DNA based diagnostics to the extent that a whole bacterial genome can be sequenced within a week.…”

Section: Genosensorsmentioning

confidence: 99%

Developments in nanoparticles for use in biosensors to assess food safety and quality

Warriner

Reddy

Namvar

et al. 2014

Trends in Food Science & Technology

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Highlights•Background to the evolution of biosensor based on nanoparticles.•Application of nanoparticles in biosensor devices.•Overview of biosensor formats applied in food testing.•Examples of integrated biosensor systems for food sampling.•Prospects and challenges in nanosensors in pathogen testing.The following will provide an overview on how advances in nanoparticle technology have contributed towards developing biosensors to screen for safety and quality markers associated with foods. The novel properties of nanoparticles will be described and how such characteristics have been exploited in sensor design will be provided. All the biosensor formats were initially developed for the health care sector to meet the demand for point-of-care diagnostics. As a consequence, research has been directed towards miniaturization thereby reducing the sample volume to nanolitres. However, the needs of the food sector are very different which may ultimately limit commercial application of nanoparticle based nanosensors. IntroductionThrough evolution we have developed senses to enable us to differentiate good foods from bad. The art of cheese making, brewing and viticulture has relied on the producer using their senses to assess quality. Establishing if a food was safe was more problematic and typically relied on tasters to ensure dishes provided to their masters were sound. Clearly, analytical testing is a more reliable and objective means of assessing the quality and safety of foods. Laboratory testing provided a basis for developing protocols and limits that could be applied to foods to define quality or safety. Yet, the expense and delay in acquiring results remain critical limitations. Consequently, there is an on-going demand for biosensors that can be used outside the laboratory environment to assess the safety and quality of foods on-site.In many instances the needs of the food industry mirrored that of the health sector where rapid diagnostics would enhance treatments and prognosis. The health sector provides a more lucrative market for diagnostic companies so it is not unexpected to find that the majority of research to date has been towards developing biosensors to meet the market demands. Consequently, those biosensors encountered in the food sector are derived from those devices initially fabricated to meet the needs of the health care sector. Within this context, the following review provides a brief history on how the field of biosensors developed and the contribution made by advances in nanoparticle technology. The efforts in transferring biosensor technologies to the food sector will be provided with a focus on safety, and to a degree, quality assessment. The review does not attempt to provide an exhaustive list of advances given the numerous quality reviews are already published in this area (Arugula and Simonian,

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Detection and identification of anisakids in seafood by fragment length polymorphism analysis and PCR–RFLP of ITS-1 region

Cited by 18 publications

References 48 publications

Occurrence and prevalence of fish-borne Anisakis larvae in the spotted mackerel Scomber australasicus from Taiwanese waters

Occurrence and prevalence of fish-borne Anisakis larvae in the spotted mackerel Scomber australasicus from Taiwanese waters

Identification of Smallanthus sonchifolius in herbal tea mixtures by PCR and DART/TOF-MS methods

Developments in nanoparticles for use in biosensors to assess food safety and quality

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