A method of printing uniform protein lines by using flat PDMS stamps

Zhang, Wei; Xue, Changying; Yang, Kun‐Lin

doi:10.1016/j.jcis.2010.09.029

Cited by 7 publications

(5 citation statements)

References 34 publications

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“…[25][26][27][28] Taking advantage of the low surface energy of PDMS, 15 similar procedures have been used to produce patterns and arrays by exposing the surface of this material to target proteins. [29][30][31][32][33][34] In this regard, controlling not only the amount of adsorbed protein, but also the orientation and conformation of the protein layer is particularly important when proteins (such as fibronectin 35 ) mediate interactions with other biological entities such as cells. [36][37][38][39][40] Despite the advantages and the intriguing nature of the studies reported in literature, only few research groups 41,42 have investigated the influence of adsorption kinetics on the biological activity of proteins adsorbed to PDMS.…”

Section: Introductionmentioning

confidence: 99%

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

et al. 2011

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This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.

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Section: Introductionmentioning

confidence: 99%

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

et al. 2011

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“…Because of the lower concentration, Nitrogen cannot have an appreciable influence on the argon metastable states processes populating. However, it can efficiently depopulate these states in the reaction: , In the working gas, the Arm concentration drops with Nitrogen concentration increasing [9][10][11][12]. As the Nitrogen is lower, the quantity of argon metastable states is enough to excite the Nitrogen molecules to give out UV.…”

Section: Resultsmentioning

confidence: 99%

Flat Panel Ultrovilet Lamps by vehicle of Porous Alumina and ITO glass

Li¹,

Wang²,

Wang³

2012

Proceedings of the 2nd International Conference on Electronic and Mechanical Engineering and Information Technology (2012)

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“…The PDMS surface was then moved to an open glass tube equipped with a UV pen lamp (Spectronics, model 11SC-1, 254 nm) for UV exposure. The distance between the stamp and the lamp is around 1.5 cm. − The power of the UV pen lamp is 4500 μW/cm 2 , and it provides 1.89 J/cm 2 during 7 min of exposure time. In this configuration, only square regions, which were not shielded by metal bars of the TEM grid, were exposed to UV.…”

Section: Methodsmentioning

confidence: 99%

Simplest Method for Creating Micropatterned Nanostructures on PDMS with UV Light

et al. 2011

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The fabrication of micropatterned structures on PDMS is a critical step in soft lithography, microfluidics, and many other PDMS-based applications. To substitute traditional mold-casting methods, we develop a simple method to create micropatterned nanostructures on PDMS in one step. After exposing a flat PDMS surface to a UV pen lamp through a photomask (such as a TEM grid), micropatterned nanostructures can be formed readily on the PDMS surface. We also demonstrate that fabricated PDMS can be used for the microcontact printing of protein immunoglobulin (IgG) on solid surfaces. This method is probably the simplest method of creating micropatterned nanostructures on PDMS reported so far because it does not need casting, surface coating, or chemical reagents. Only a UV pen lamp and a photomask are required, and this method can be performed under ambient conditions without vacuum. We expect that this method will greatly benefit researchers who use PDMS regularly in various applications such as soft lithography and microfluidics.

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A method of printing uniform protein lines by using flat PDMS stamps

Cited by 7 publications

References 34 publications

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Flat Panel Ultrovilet Lamps by vehicle of Porous Alumina and ITO glass

Simplest Method for Creating Micropatterned Nanostructures on PDMS with UV Light

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