A Method of Permanently Mounting Biological Tissue Cleared in Herr's Four-And-A-Half Clearing Fluid

Abstract

A technique which should be generally applicable for preparing permanent mounts of tissue cleared in Herr's four-and-a-half clearing fluid is described. This technique involves transferring plant or animal tissues through a series of solutions consisting of Pienarr's fixative, Herr's clearing fluid, chloral hydrate, acetone and finally polyester resin for mounting. Material prepared using this method is exceptionally transparent and well preserved, and is suitable for either phase contrast or Nomarski interference microscopy.

A revision of Dodonaea Miller (Sapindaceae) in Australia.

“…In order to examine the developmental stages of the seed of some Dodonaea species and so to determine the origin of the a d , I attempted to clear material ranging from young ovules to almost mature seeds by the method of Blackburn and Christophel (1976). However, it was not possible to obtain cleared ovules and without cellular detail it is difficult to determine whether the majority of Dodonaea species which appear to lack any aril do possess a small funicular aril as described by Corner (1976) for D. viscosa.…”