A method for the molecular imprinting of hemoglobin on silica surfaces using silanes

Shiomi, Toru; Matsui, Masanao; Mizukami, Fujio; Sakaguchi, Kengo

doi:10.1016/j.biomaterials.2005.02.007

Cited by 227 publications

(160 citation statements)

References 27 publications

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“…Shiomi imprinted porous silica with hemoglobin (Hb) by covalently immobilizing it to the silica using imine bonds (75). After imprinting the protein was removed by an oxalic acid wash, to break the covalent attachments.…”

Section: Dmentioning

confidence: 99%

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From 3D to 2D: A Review of the Molecular Imprinting of Proteins

Turner¹,

Jeans²,

Brain³

et al. 2006

Biotechnol. Prog.

186

217

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Molecular imprinting is a generic technology that allows for the introduction of sites of specific molecular affinity into otherwise homogeneous polymeric matrices. Commonly this technique has been shown to be effective when targeting small molecules of molecular weight <1500, while extending the technique to larger molecules such as proteins has proven difficult. A number of key inherent problems in protein imprinting have been identified, including permanent entrapment, poor mass transfer, denaturation, and heterogeneity in binding pocket affinity, which have been addressed using a variety of approaches. This review focuses on protein imprinting in its various forms, ranging from conventional bulk techniques to novel thin film and monolayer surface imprinting approaches.

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Section: Dmentioning

confidence: 99%

“…Further examples of each technique are required, using a wider range of proteins. The potential demonstrated with sol-gels is high (93), but as yet only three protocols have been reported (60,62,75).…”

Section: Conclusion and Future Outlookmentioning

confidence: 99%

From 3D to 2D: A Review of the Molecular Imprinting of Proteins

Turner¹,

Jeans²,

Brain³

et al. 2006

Biotechnol. Prog.

186

217

View full text Add to dashboard Cite

show abstract

“…Nevertheless, examples of molecularly imprinted materials for biomolecular recognition have been developed for proteins [18][19][20][21][22][23][24][25] . The most convincing examples had been developed using surfaceimprinting approaches.…”

mentioning

confidence: 99%

A synthetic nanomaterial for virus recognition produced by surface imprinting

et al. 2013

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Major stumbling blocks in the production of fully synthetic materials designed to feature virus recognition properties are that the target is large and its self-assembled architecture is fragile. Here we describe a synthetic strategy to produce organic/inorganic nanoparticulate hybrids that recognize non-enveloped icosahedral viruses in water at concentrations down to the picomolar range. We demonstrate that these systems bind a virus that, in turn, acts as a template during the nanomaterial synthesis. These virus imprinted particles then display remarkable selectivity and affinity. The reported method, which is based on surface imprinting using silica nanoparticles that act as a carrier material and organosilanes serving as biomimetic building blocks, goes beyond simple shape imprinting. We demonstrate the formation of a chemical imprint, comparable to the formation of biosilica, due to the template effect of the virion surface on the synthesis of the recognition material.

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“…11,12 MIPs have been made with various types of monomers, mostly of which are acrylic and silane based. 13,14 However, most MIPs suffer from poor binding affinity or specificity. 15,16 At the same time, it is difficult to produce a fluorescence signal for binding.…”

Section: Introductionmentioning

confidence: 99%

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

Zhang

Liu

2016

ACS Appl. Mater. Interfaces

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Molecularly imprinted polymers (MIPs) are produced in the presence of a template molecule. After removing the template, the cavity can selectively rebind the template. MIPs are attractive functional materials with a low cost and high stability, but traditional MIPs often suffer from low binding affinity. This study employs DNA aptamer fragments as macromonomers to improve MIPs. The DNA aptamer for adenosine was first split into two halves, fluorescently labeled, and co-polymerized into MIPs. With a fluorescence quenching assay, the importance of imprinting was confirmed. Further studies were carried out using isothermal titration calorimetry (ITC).Compared to the mixture of the free aptamer fragments, their MIPs doubled the binding affinity.Each free aptamer fragment alone cannot bind adenosine, while MIPs containing each fragment are effective binders. We further shortened one of the aptamer fragments, and the DNA length was pushed to as short as six nucleotides, yielding MIPs with a dissociation constant of 27 µM adenosine. This study provides a new method for preparing functional MIP materials by combining high affinity biopolymer fragments with low cost synthetic monomers, allowing higher binding affinity and providing a method for signaling binding based on DNA chemistry.

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A method for the molecular imprinting of hemoglobin on silica surfaces using silanes

Cited by 227 publications

References 27 publications

From 3D to 2D: A Review of the Molecular Imprinting of Proteins

From 3D to 2D: A Review of the Molecular Imprinting of Proteins

A synthetic nanomaterial for virus recognition produced by surface imprinting

Molecularly Imprinted Polymers with DNA Aptamer Fragments as Macromonomers

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