A method for the culture of lichen thalli under controlled conditions

Galun, Margalith; Marton, Kela; Behr, Leah

doi:10.1007/bf00645120

Cited by 15 publications

(4 citation statements)

References 4 publications

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“…Lichens are reputedly slow growing, difficult to grow indoors or in growth chambers, and apt to become unhealthy or die in response to supplements of the usual limiting resources, such as water or nitrogen (Galun et al 1972, Bubrick 1988, Bando and Sugino 1995. ''Overirrigation or excess nutrients usually leads to death or dissolution of the symbiosis'' (Bubrick 1988:127).…”

Section: Introductionmentioning

confidence: 99%

A single phosphorus treatment doubles growth of cyanobacterial lichen transplants

McCune¹,

Caldwell²

2009

Ecology

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Lichens are reputedly slow growing and become unhealthy or die in response to supplements of the usual limiting resources, such as water and nitrogen. We found, however, that the tripartite cyanobacterial lichen Lobaria pulmonaria doubled in annual biomass growth after a single 20-minute immersion in a phosphorus solution (K2HPO4), as compared to controls receiving no supplemental phosphorus. This stimulation of cyanolichens by phosphorus has direct relevance to community and population ecology of lichens, including improving models of lichen performance in relation to air quality, improving forest management practices affecting old-growth associated cyanolichens, and understanding the distribution and abundance of cyanolichens on the landscape. Phosphorus may be as important a stimulant to cyanobacterial-rich lichen communities as it is to cyanobacteria in aquatic ecosystems.

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Section: Introductionmentioning

confidence: 99%

A single phosphorus treatment doubles growth of cyanobacterial lichen transplants

McCune¹,

Caldwell²

2009

Ecology

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“…If reclamation scientists could test treatments in a growth chamber, that could allow them to optimize limited field time in the arctic. To date, only four studies have assessed lichen biocrust survival in a controlled environment for reclamation purposes (Maestre et al 2006; Bowker & Antoninka 2016; Antoninka et al 2018; Bowker et al 2020), and eight studies have examined optimal growth chamber conditions for field‐collected lichen species (Kershaw & Millbank 1969; Dibben 1971; Galun et al 1972; Bidussi et al 2013; Bu et al 2013; Antoninka et al 2015; Gauslaa et al 2016; Zhao et al 2016). The majority of this work has been done in lower latitude dryland systems and none of these studies have assessed growth chamber conditions for Arctic lichen biocrusts.…”

Section: Introductionmentioning

confidence: 99%

“…Of the studies on lichens and lichen dominated biocrusts, common inoculation techniques in the field and growth chamber included selecting individual species, transplanting intact crust pieces, or artificial fragmentation of crust material (sieving, pulverization, or wet slurry) (Maestre et al 2006; Roturier et al 2007; Bowker et al 2020). Growth chamber watering regimes varied from daily, every few days, to once a month, with several studies emphasizing the importance of alternating wet and dry periods (Galun et al 1972; Maestre et al 2006; Antoninka et al 2018). Lichens are sensitive to substrate properties including pH, texture, and nutrients (Robinson et al 1989; Belnap & Eldridge 2001; Bowker et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Optimizing growth chamber conditions for maintaining Arctic lichen‐dominated biocrusts

Ficko

McClymont²,

Haughland

et al. 2023

Restoration Ecology

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Optimizing growth chamber conditions for Arctic lichen biocrusts will create new opportunities to assess and prioritize reclamation techniques given the challenges associated with conducting arctic field work. Our study is the first to examine growth chamber conditions for optimizing survival and growth of Arctic lichen biocrusts, as measured by changes in lichen cover. We assessed effects of substrate crossed with substrate depth, substrate sterilization, lichen inoculation and community composition, and watering frequency in four concurrent experiments over 6 weeks on survival of arctic biocrusts collected from Diavik Diamond Mine Inc., Northwest Territories, Canada. Mixed species declined less than Flavocetraria cucullata, and substrate affected F. cucullata survival over time. Live lichen cover declined least with a 3-day watering frequency and substrate depth of 1 cm. Sterilization did not affect lichen survival, and no contamination was observed over 6 weeks. Our results highlight the challenges of maintaining and growing lichens under controlled conditions, as only a few treatments showed increases in cover. Our research shows that even short-term growth chamber experiments have potential to screen reclamation treatments prior to field assessments, permitting reclamation scientists to optimize limited time and resources while in the field.

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“…Few investigations have referred to resynthesis experiments with cyanobacterial lichens (e.g. Reess 1871, Galun et al 1972, Marton & Galun 1976, Ahmadjian 1989. Degelius (1954) attempted to synthesise Collema species on different substrata such as clay and silica gel, but without success.…”

Section: Introductionmentioning

confidence: 99%

Artificial Resynthesis of Thalli of the Cyanobacterial Lichen Peltigera Praetextata Under Laboratory Conditions

Stocker-Wörgötter

Türk

1991

The Lichenologist

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The resynthesis of foliose thalli of the cyanobacterial lichen Peltigera praetextata was achieved on a soil substratum under laboratory conditions. On agar plates mycelia formation by this lichen fungus only occurred when a sufficient number of photobiont cells was present. Resynthesis on agar was restricted to the formation of loose algal-fungal associations, which showed no lichen-like structures. Early resynthesis stages were formed on soil only after a very long lag phase of 2–3 months. First soredia-like stages (resynthesis products of fungal hyphae and cyanobacteria) appeared, later tissue clumps and thallus primordia differentiated. Welldeveloped lichen thalli with rhizines that resembled young thalli from the natural environment were formed after an incubation time of 2–3 years.

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A method for the culture of lichen thalli under controlled conditions

Cited by 15 publications

References 4 publications

A single phosphorus treatment doubles growth of cyanobacterial lichen transplants

A single phosphorus treatment doubles growth of cyanobacterial lichen transplants

Optimizing growth chamber conditions for maintaining Arctic lichen‐dominated biocrusts

Artificial Resynthesis of Thalli of the Cyanobacterial Lichen Peltigera Praetextata Under Laboratory Conditions

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