Summary
Germinated wheat and barley were extruded with a pilot‐scale corotating twin‐screw extruder under different barrel temperatures (100 and 120 °C), feed moisture content (30 and 35%) and CO2 injection. The effect of germination, barrel temperature, feed moisture content and CO2 injection on physical properties, proximate composition, γ‐aminobutyric acid, β‐glucan, phytic acid and protease activity was investigated. Results indicated that the barrel temperature, moisture content and CO2 injection significantly affected water absorption index (WAI), water‐soluble solid index (WSI), specific length, expansion ratio, bulk density, mechanical properties and colour of all extruded products (P < 0.05). Extrusion process significantly decreased γ‐aminobutyric acid and protease activity in extruded germinated wheat (EGW) and barley (EGB) products (with and without CO2 injection). However, extrusion process (with and without CO2 injection) increased β‐glucan in EGB and decreased phytic acid in EGW. The content of proximate composition, γ‐aminobutyric acid, β‐glucan, phytic acid and protease activity was slightly affected by CO2 injection.