A Method for Patterning Multiple Types of Cells by Using Electrochemical Desorption of Self‐Assembled Monolayers within Microfluidic Channels

Abstract

Fenced in: Selective electrochemical desorption of self‐assembled monolayers of HS(CH2)11(OCH2CH2)6OH was carried out on parts of a gold substrate restricted to microfluidic channels. This chemical transformation activates parts of the surface for adhesion of multiple types of cells with well‐controlled geometry.

A Strategy for Depositing Different Types of Cells in Three Dimensions to Mimic Tubular Structures in Tissues

“…Furthermore, precise control of cell location and orientation in tubular structures is still an unresolved problem. [11] Herein, our strategy circumvents these limitations. This strategy has two steps: i) delivering and patterning different types of cells on a 2D SIRM using microfluidic channels; [12][13][14] ii) releasing the SIRM to roll up into a 3D tube.…”

Surface Coating as a Key Parameter in Engineering Neuronal Network Structures In Vitro

“…We further studied how astrocytes could affect the polarized morphology of neurons. While there is a number of ways for patterning multiple types of cells controllably on the same substrate [52,53], the specific morphology of neural cells that evolve polarized morphology after adhesion makes it essential to develop new methods for culturing multiple neural cells together. Through the combination of lCP with microfluidic channels (Fig.…”

Controllable Patterning of Different Cells Via Optical Assembly of 1D Periodic Cell Structures

“…Compared with the previously reported cell patterning methods, such as microcontact printing, [ 8 ] lithography, [ 9 ] inkjet printing, [ 10 ] dielectrophoresis, [ 11 ] and electrochemical desorption, [ 12 ] the main advantages of our method are 1) the method is very simple with only a single ATF and a laser source, without the need of elaborately fabricated substrates of electrodes to predetermine the locations of cell patterns; 2) the patterning offers single-cell precision and control; 3) cells of different types can be controllable positioned into designated locations, forming cell structures with periodic confi gurations via direct cell-cell contact; 4) the patterned cell structures can be fl exibly moved in 3D to designated locations; 5) the optical signal propagating along the cell structures can be detected in real-time. Compared with the optical trapping method using optical tweezers for cell patterning by trapping multiple cells, [ 16 ] the main advantages of this method are setup simplicity and manipulation fl exibility.…”

“…[ 6,7 ] Until now, different techniques have demonstrated the ability for cell patterning by immobilizing cells on designated regions of a surface. For example, cell patterning has been achieved by using microcontact printing [ 8 ] or lithography [ 9 ] to defi ne the locations of cell attachment, inkjet printing [ 10 ] to place cells pixel by pixel on a substrate, dielectrophoresis [ 11 ] to use dielectrophoretic forces to concentrate cells into specifi c locations, and electrochemical desorption [ 12 ] to use electrochemically constrained surface changes to attach cells. In addition, by cooperating with microcontact printing, orthogonal engineering matrix can be used to regulate multicellular morphology, which suggests important ways in developing implantable materials via cell patterning.…”