A Method for Isolating Total RNA from Mature Buds and Other Woody Tissues of Vitis Vinifera

Journal of Experimental Botany

Shi

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Distinct gibberellin functions during and after grapevine bud dormancy release

Zheng

Journal of Experimental Botany

Shi

et al. 2018

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“…Total RNA was extracted using the CTAB protocol as previously described ( Acheampong et al , 2010 ). First-strand cDNA was synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Promega Corporation, Madison, WI, USA) according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera

Journal of Experimental Botany

Rotman

et al. 2015

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“…Total RNA extraction from finely ground powder originating from three young leaves or 20 frozen buds was done using the CTAB (cetyltrimethyl ammonium bromide) protocol (Chang, Puryear, & Cairney, 1993), with modifications as previously described (Acheampong et al, 2010). Complementary DNA was synthesized from total RNA using the M-MLV Reverse Transcriptase Kit as detailed in the manufacturer's manual (Promega, Madison, WI, USA).…”

Section: Nucleic Acid Extractionmentioning

confidence: 99%

Abscisic acid catabolism enhances dormancy release of grapevine buds

Zheng

Plant Cell & Environment

Shi

et al. 2018

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The molecular mechanism regulating dormancy release in grapevine buds is as yet unclear. It was formerly proposed that dormancy is maintained by abscisic acid (ABA)-mediated repression of bud-meristem activity and that removal of this repression triggers dormancy release. It was also proposed that such removal of repression may be achieved via natural or artificial up-regulation of VvA8H-CYP707A4, which encodes ABA 8'-hydroxylase, and is the most highly expressed paralog in grapevine buds. The current study further examines these assumptions, and its experiments reveal that (a) hypoxia and ethylene, stimuli of bud dormancy release, enhance expression of VvA8H-CYP707A4 within grape buds, (b) the VvA8H-CYP707A4 protein accumulates during the natural transition to the dormancy release stage, and (c) transgenic vines overexpressing VvA8H-CYP707A4 exhibit increased ABA catabolism and significant enhancement of bud break in controlled and natural environments and longer basal summer laterals. The results suggest that VvA8H-CYP707A4 functions as an ABA degrading enzyme, and are consistent with a model in which the VvA8H-CYP707A4 level in the bud is up-regulated by natural and artificial bud break stimuli, which leads to increased ABA degradation capacity, removal of endogenous ABA-mediated repression, and enhanced regrowth. Interestingly, it also hints at sharing of regulatory steps between latent and lateral bud outgrowth.