Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera

Acheampong, Atiako Kwame; Hu, Jianhong; Rotman, Ariel; Zheng, Chuanlin; Halaly, Tamar; Takebayashi, Yumiko; Kamiya, Yûji; Lichter, Amnon; Sun, Tai-ping; Or, Etti

doi:10.1093/jxb/eru504

Cited by 38 publications

(63 citation statements)

References 59 publications

(85 reference statements)

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“…GA orchestrates a broad range of processes and many levels of regulation are known to be involved in determining GA-responses, including biosynthesis, metabolism and signalling [8, 78]. Our data and those of others [11, 79] showed that another level of regulation exists in terms of GA–GID1–DELLA binding capacity and preference.…”

Section: Resultssupporting

confidence: 71%

“…Conserved DELLA domain is essential for GA-GID1–DELLA interaction, since any deletion or point substitution results in loss-of-interaction ability despite the presence of GA [47, 49, 58, 75]. Recently, a grape DELLA protein, VvDELLA3, exhibiting high sequence similarity to PslRGA has been characterized [11]. Although, both VvDELLA3 and PslRGA share the disrupted DELLA domain, VvDELLA3 displayed selective interaction with VvGID1-like proteins.…”

Section: Resultsmentioning

confidence: 99%

“…Gibberellin (GA) is an essential hormone involved in diverse biological processes, leading to correct plant growth and development [5–8]. In tree fruit species, the proper establishment of reproductive growth is dependent on coordinated levels of GA at the appropriate developmental stages [9–11]. Application of GA resulted in visible improvement of fruit quality traits in terms of size, weight and many other characteristics [12–13].…”

Section: Introductionmentioning

confidence: 99%

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Plum Fruit Development Occurs via Gibberellin–Sensitive and –Insensitive DELLA Repressors

et al. 2017

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Fruit growth depends on highly coordinated hormonal activities. The phytohormone gibberellin (GA) promotes growth by triggering degradation of the growth-repressing DELLA proteins; however, the extent to which such proteins contribute to GA-mediated fruit development remains to be clarified. Three new plum genes encoding DELLA proteins, PslGAI, PslRGL and PslRGA were isolated and functionally characterized. Analysis of expression profile during fruit development suggested that PslDELLA are transcriptionally regulated during flower and fruit ontogeny with potential positive regulation by GA and ethylene, depending on organ and developmental stage. PslGAI and PslRGL deduced proteins contain all domains present in typical DELLA proteins. However, PslRGA exhibited a degenerated DELLA domain and subsequently lacks in GID1–DELLA interaction property. PslDELLA–overexpression in WT Arabidopsis caused dramatic disruption in overall growth including root length, stem elongation, plant architecture, flower structure, fertility, and considerable retardation in development due to dramatic distortion in GA-metabolic pathway. GA treatment enhanced PslGAI/PslRGL interaction with PslGID1 receptors, causing protein destabilization and relief of growth-restraining effect. By contrast, PslRGA protein was not degraded by GA due to its inability to interact with PslGID1. Relative to other PslDELLA–mutants, PslRGA–plants displayed stronger constitutive repressive growth that was irreversible by GA application. The present results describe additional complexities in GA-signalling during plum fruit development, which may be particularly important to optimize successful reproductive growth.

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Section: Resultssupporting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Plum Fruit Development Occurs via Gibberellin–Sensitive and –Insensitive DELLA Repressors

et al. 2017

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“…The transcript levels of VvGID1 s, VvDELLA s, VvSLY1 s, and VvGASA s were measured by quantitative real-time PCR (qRT-PCR) as previously described (Acheampong et al, 2015), while and VvGA2ox s, VvGA3ox s, and VvGA20ox s expressions were by qRT-PCR using EvaGreen DNA-binding dye (Biotium Inc., Hayward, CA, United States) on the 96.96 Dynamic Array Integrated Fluidic Circuits (IFCs) (Fluidigm, San Francisco, CA, United States). Expressions of genes were normalized to transcripts of previously characterized, non-GA regulated VvGAPDH (Reid et al, 2006; Giacomelli et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

“…Full-length VvDELLA proteins, used as sizing standards to locate endogenous VvDELLA proteins, were also expressed and purified as previously described (Acheampong et al, 2015), and quantified using BSA standards.…”

Section: Methodsmentioning

confidence: 99%

Abnormal Endogenous Repression of GA Signaling in a Seedless Table Grape Cultivar with High Berry Growth Response to GA Application

et al. 2017

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Gibberellin (GA) application is routinely used in the table grape industry to increase berry size and cluster length. Although grapevine cultivars show a wide range of growth responsiveness to GA3 application, the reasons for these differences is unclear. To shed light on this issue, two commercial grapevine cultivars with contrasting berry response to GA were selected for comparative analysis, in which we tested if the differences in response: (1) is organ-specific or cultivar-related; (2) will be reflected in qualitative/quantitative differences in transcripts/proteins of central components of GA metabolism and signaling and levels of GA metabolites. Our results showed that in addition to the high response of its berries to GA, internodes and rachis of cv. Black finger (BF) presented a greater growth response compared to that of cv. Spring blush (SB). In agreement, the results exposed significant quantitative differences in GA signaling components in several organs of both cultivars. Exceptionally higher level of all three functional VvDELLA proteins was recorded in young BF organs, accompanied by elevated VvGID1 expression and lower VvSLY1b transcripts. Absence of seed traces, low endogenous GA quantities and lower expression of VvGA20ox4 and VvGA3ox3 were also recorded in berries of BF. Our results raise the hypothesis that, in young organs of BF, low expression of VvSLY1b may be responsible for the massive accumulation of VvDELLA proteins, which then leads to elevated VvGID1 levels. This integrated analysis suggests causal relationship between endogenous mechanisms leading to anomalous GA signaling repression in BF, manifested by high quantities of VvDELLA proteins, and greater growth response to GA application.

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Regulatory mechanism of GA3 on tuber growth by DELLA-dependent pathway in yam (Dioscorea opposita)

Zhou

Gong

et al. 2021

Plant Mol Biol

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Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera

Abstract: HighlightCharacterization of grape GA signalling components and GA quantitation unveil the berry-size regulating DELLA, and show that differential organ responses stem from both VvDELLAs and endogenous GA levels.

Cited by 38 publications

References 59 publications

Plum Fruit Development Occurs via Gibberellin–Sensitive and –Insensitive DELLA Repressors

Plum Fruit Development Occurs via Gibberellin–Sensitive and –Insensitive DELLA Repressors

Abnormal Endogenous Repression of GA Signaling in a Seedless Table Grape Cultivar with High Berry Growth Response to GA Application

Regulatory mechanism of GA3 on tuber growth by DELLA-dependent pathway in yam (Dioscorea opposita)

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