A method for estimation of plasma albumin concentration from the buffering properties of whole blood

Rees, Stephen Edward; Diemer, Tue; Kristensen, Søren Risom

doi:10.1016/j.jcrc.2012.02.011

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…Also Eq 7 , being based on the modeling of the albumin effect as described by Watson [ 13 ], assumes control of pH dependent interaction between albumin and calcium and magnesium. Although the free charge on albumin has been used to measure the concentration of albumin [ 28 ] we cannot assume this always to be straightforward. As an example, the binding of calcium to albumin has been found to decrease in uremia [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Strong Relationships in Acid-Base Chemistry – Modeling Protons Based on Predictable Concentrations of Strong Ions, Total Weak Acid Concentrations, and pCO2

Ring¹,

Kellum

2016

PLoS ONE

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Understanding acid-base regulation is often reduced to pigeonholing clinical states into categories of disorders based on arterial blood sampling. An earlier ambition to quantitatively explain disorders by measuring production and elimination of acid has not become standard clinical practice. Seeking back to classical physical chemistry we propose that in any compartment, the requirement of electroneutrality leads to a strong relationship between charged moieties. This relationship is derived in the form of a general equation stating charge balance, making it possible to calculate [H+] and pH based on all other charged moieties. Therefore, to validate this construct we investigated a large number of blood samples from intensive care patients, where both data and pathology is plentiful, by comparing the measured pH to the modeled pH. We were able to predict both the mean pattern and the individual fluctuation in pH based on all other measured charges with a correlation of approximately 90% in individual patient series. However, there was a shift in pH so that fitted pH in general is overestimated (95% confidence interval -0.072–0.210) and we examine some explanations for this shift. Having confirmed the relationship between charged species we then examine some of the classical and recent literature concerning the importance of charge balance. We conclude that focusing on the charges which are predictable such as strong ions and total concentrations of weak acids leads to new insights with important implications for medicine and physiology. Importantly this construct should pave the way for quantitative acid-base models looking into the underlying mechanisms of disorders rather than just classifying them.

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Section: Discussionmentioning

confidence: 99%

Strong Relationships in Acid-Base Chemistry – Modeling Protons Based on Predictable Concentrations of Strong Ions, Total Weak Acid Concentrations, and pCO2

Ring¹,

Kellum

2016

PLoS ONE

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“…The absence of observation of food intake was a potential limitation in our study. Evidence suggested that trauma is believed to affect animal food intake [ 3 ]. However, no meta-analysis indicated that food intake was associated with the gastrointestinal motility so far.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing evidence shows that TBI can lead to several physiologic complications including gastrointestinal dysfunction [ 2 ]. Traumatic brain injury causes a delayed but significant decrease in intestinal contractile activity, which is associated with disturbances in food intake and inadequate nutrition [ 3 ]. Therefore, development of optimal treatment strategies to improve intestinal motility has important clinical significance.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Lactobacillus acidophilus on the Intestinal Smooth Muscle Contraction through PKC/MLCK/MLC Signaling Pathway in TBI Mouse Model

Sun¹,

Hu²,

Fang³

et al. 2015

PLoS ONE

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Clinical studies have shown that probiotics influence gastrointestinal motility. However, the molecular mechanisms by which probiotic Lactobacillus modulates intestinal motility in traumatic brain injury (TBI) mouse model have not been explored. In the present study, we provided evidence showing that treatment of TBI mice with Lactobacillus acidophilus significantly improved the terminal ileum villus morphology, restored the impaired interstitial cells of Cajal (ICC) and the disrupted ICC networks after TBI, and prevented TBI-mediated inhibition of contractile activity in intestinal smooth muscle. Mechanistically, the decreased concentration of MLCK, phospho-MLC20 and phospho-MYPT1 and increased concentration of MLCP and PKC were observed after TBI, and these events mediated by TBI were efficiently prevented by Lactobacillus acidophilus application. These findings may provide a novel mechanistic basis for the application of Lactobacillus acidophilus in the treatment of TBI.

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“…Considering that HDL also has the ability to bind to S1P, HDL is likely to inhibit S1P uptake in the same manner as BSA. Albumin concentration in human plasma is 4% to 5% ( 43 ). MEDEP-E14 cells exhibited S1P uptake activity even in the presence of 10% BSA ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Direct uptake of sphingosine-1-phosphate independent of phospholipid phosphatases

Goto

Miyamoto

Kihara

2021

Journal of Biological Chemistry

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Sphingosine-1-phosphate (S1P) is a lipid mediator that is relatively abundant in plasma and plays an important role in the vascular and immune systems. To date, the only known mechanism for removing S1P from plasma has been dephosphorylation by phospholipid phosphatases (PLPPs) on the surface of cells in contact with the plasma. However, there remains a possibility that PLPP-independent dephosphorylation or direct S1P uptake into cells could occur. To examine these possibilities, here we generated triple KO (TKO) HAP1 cells that lacked all PLPPs ( PLPP1–3 ) present in mammals. In the TKO cells, the intracellular metabolism of externally added deuterium-labeled S1P to ceramide was reduced to 17% compared with the WT cells, indicating that most extracellular S1P is dephosphorylated by PLPPs and then taken up into cells. However, this result also reveals the existence of a PLPP-independent S1P uptake pathway. Tracer experiments using [ 32 P]S1P showed the existence of a direct S1P uptake pathway that functions without prior dephosphorylation. Overexpression of sphingolipid transporter 2 (SPNS2) or of major facilitator superfamily domain containing 2B (MFSD2B), both known S1P efflux transporters, in TKO cells increased the direct uptake of S1P, whereas KO of MFSD2B in TKO cells reduced this uptake. These results suggest that these are channel-type transporters and capable of not only exporting but also importing S1P. Furthermore, we observed that erythroid cells expressing MFSD2B, exhibited high S1P uptake activity. Our findings describing direct S1P uptake may contribute to the elucidation of the molecular mechanisms that regulate plasma S1P concentration.

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A method for estimation of plasma albumin concentration from the buffering properties of whole blood

Cited by 4 publications

References 16 publications

Strong Relationships in Acid-Base Chemistry – Modeling Protons Based on Predictable Concentrations of Strong Ions, Total Weak Acid Concentrations, and pCO2

Strong Relationships in Acid-Base Chemistry – Modeling Protons Based on Predictable Concentrations of Strong Ions, Total Weak Acid Concentrations, and pCO2

The Effects of Lactobacillus acidophilus on the Intestinal Smooth Muscle Contraction through PKC/MLCK/MLC Signaling Pathway in TBI Mouse Model

Direct uptake of sphingosine-1-phosphate independent of phospholipid phosphatases

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