A Method for Estimating Volume-Surface Ratios

Chalkley, H. W.; Cornfield, Jerome; Park, Helen

doi:10.1126/science.110.2856.295

Cited by 198 publications

(56 citation statements)

References 1 publication

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“…1) on the cell V/A a morphometric analysis (Chalkley, Cornfield & Park, 1949) (Glvnn, 19056) and involved centrifugation and resuspension of the cells. After loading with isotope, three washes were carried out to clear the extracellullar activity; each took 3 min.…”

Section: Culture Methodsmentioning

confidence: 99%

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Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Lamb¹,

Mackinnon²

1971

The Journal of Physiology

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SUMMARY1. The general characteristics and Na and K movements of L cells (derived from mouse epithelium) have been measured. Both cells grown in suspension (LS cells) and as a monolayer (L cells) were used.2. The volume of L cells was 1-2 x 10-9 cm3 and of LS cells 3-5 x 10-9 cm3; of this 82 % was water.3. Electron micrographs showed the presence of numerous protrusions (filopodia) from both forms of the cell. These had the effect of increasing the surface area of the cell by 2-4 times over smooth cells of the same volume. On changing from the flattened to the spherical shape during trypsinization, the filopodia altered to maintain a constant V/A ratio. 6. Both DNP (10-3M) and IAA (IO 4M) are required for maximum inhibition of K uptake, as both aerobic and anaerobic metabolic pathways may be used to drive the pump.7. K removal decreases the Na efflux, and Na removal (eventually) decreases the K influx providing evidence for Na/K coupling.

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Section: Culture Methodsmentioning

confidence: 99%

“…These 'filopodia' had the effect of greatly increasing the surface area (roughly x 2) with only a small increase in volume (< 5 %). From a morphometric analysis of these sections (Chalkley et al 1949) (Table 1). The following points may therefore be made.…”

Section: Estimation Of Nucleotides In L Cellsmentioning

confidence: 99%

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Lamb¹,

Mackinnon²

1971

The Journal of Physiology

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“…Quantitative morphological analysis was carried out according to the methods of Chalkley (10,11) and others (12,13).…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural Bases for Metabolically Linked Mechanical Activity in Mitochondria

Hackenbrock

1968

The Journal of Cell Biology

482

148

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Isolated mitochondria are capable of undergoing dramatic reversible ultrastructural transformations between a condensed and an orthodox conformation. These two conformations are the extremes in ultrastructural organization between which structually and functionally intact mitochondria transform during reversible respiratory cycles. It has been found that electron transport is required for the condensed-to-orthodox ultrastructural transformation which occurs in mitochondria under State IV conditions, i.e., under conditions in which exogenous substrate is present and ADP is deficient. Inhibition of State IV electron transport at the cyanide-, antimycin A-, or Amytal-sensitive sites in the respiratory chain results in inhibition of this transformation. Resumption of electron transport in initially inhibited mitochondrial systems, initiated by channeling electrons through pathways which bypass the inhibited sites, results in resumption of the ultrastructural transformation. The condensed-toorthodox transformation is DNP insensitive and, therefore, does not require participation of the coupling enzymes of the energy-transfer pathway. It is concluded that this ultrastructural transformation is manifest by the conversion of the chemical energy of electron transport directly into mechanical work. The reversed ultrastructural transformation, i.e., orthodoxto-condensed, which occurs during ADP-activated State III electron transport, is inhibited by DNP and parallels suppression of acceptor control and oxidative phosphorylation. Mechanochemical ultrastructural transformation as a basis for energy transfer in mitochondria is considered with respect to the results presented.

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“…Chord lines often cross from one alveolus through an alveolar duct to an opposite alveolus, indicating that L m characterizes the entire acinar airspace complex and not just alveoli. In simplest terms, L m is an estimator of volume-to-surface ratio of acinar airspaces (V/S) airspace , alveoli and alveolar ducts taken together: L m 5 4 Á VðairspaceÞ=SðaÞ 5 4 Á ðV=SÞ airspace (22) where V(airspace) is air volume in alveoli and ducts, and S(a) is alveolar surface area (7,24,(114)(115)(116)(117). Two methods can be used to estimate L m (118).…”

Section: Mean Linear Interceptmentioning

confidence: 99%

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

Hsia¹,

Hyde²,

Ochs³

et al. 2010

Am J Respir Crit Care Med

779

688

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A Method for Estimating Volume-Surface Ratios

Cited by 198 publications

References 1 publication

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Effect of ouabain and metabolic inhibitors on the Na and K movements of nucleotide contents of L cells

Ultrastructural Bases for Metabolically Linked Mechanical Activity in Mitochondria

An Official Research Policy Statement of the American Thoracic Society/European Respiratory Society: Standards for Quantitative Assessment of Lung Structure

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