A method for complete determination of dimethylsulphoniopropionate (DMSP) in marine macroalgae from different geographical regions

Karsten, Ulf; Kück, K.; Daniel, Claudia; Wiencke, Christian; Kirst, G. O.

doi:10.2216/i0031-8884-33-3-171.1

Cited by 31 publications

(21 citation statements)

References 4 publications

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“…We conclude that epiphytes were absent from the leaves that were analyzed and that the detected DMSP was from P. oceanica tissues and not a contamination from epiphyte residues. Due to logistic constraints, the samples for DMSP determination needed to be preserved prior to GC analysis and we applied the protocol of Karsten et al (1994) consisting on drying material for 48 h at 60 • C in an oven. Karsten et al (1994) and Van Alstyne et al (2003) showed that the DMSP content of macroalgae increases in dried material compared to fresh material, although this was not specifically tested for angiosperms such as P. oceanica.…”

Section: Discussionmentioning

confidence: 99%

“…The leaves were scrapped with a razor blade to remove epiphytes (Dauby and Poulicek 1995), and cut into sections of 10 cm starting at the base of the leaf. They were dried at 60 • C for 48 h, wrapped in aluminum foil and stored dry until further analysis, as recommended for the analysis of the DMSP content in macrophytes (Karsten et al, 1994;Van Alstyne et al, 2003). The length of the leaf is given from the base (0 cm), increasing towards the apex.…”

Section: Samplingmentioning

confidence: 99%

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Seasonal and spatial variability of dimethylsulfoniopropionate (DMSP) in the Mediterranean seagrass Posidonia oceanica

Borges

Champenois

2015

Aquatic Botany

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a b s t r a c tWe investigated for the first time the occurrence of dimethylsulfoniopropionate (DMSP) in the leaves of Posidonia oceanica and we report its variability during 4 sampling periods covering the seasonal cycle (February, June, August and November) and along a gradient from 10 m to 30 m depth. The P. oceanica leaf DMSP content expressed per mass of dry weight (dw) ranged from 0.1 to 33.9 mol g dw −1 and averaged 5.0 mol g dw −1 . It was higher than the DMSP content of roots and rhizomes that averaged ∼0.5 mol g dw −1 . The leaf DMSP content showed seasonal variations, being highest in summer when primary production and biomass of P. oceanica were also highest. In August, the leaf DMSP content showed variations with depth, increasing from 30 m to 10 m depth. In summer, the leaf DMSP content was highest in the youngest sections of leaves (closest to base) than in the older ones (closest to apex). The seasonal and depth distribution suggest that the DMSP leaf content is positively related to irradiance, hence, we hypothesize that DMSP in P. oceanica plays a role as an antioxidant against reactive oxygen species, although we cannot unambiguously exclude other potential roles such as grazer deterrent. The average leaf DMSP content of P. oceanica is modest compared to high DMSP producing macroalgae and phytoplankton. Yet, the integrated DMSP stock associated to the meadows of P. oceanica is very large due to its enormous biomass, and at the community level it is 2 orders of magnitude higher than the potential integrated DMSP stock related to phytoplankton in the same area.

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Section: Discussionmentioning

confidence: 99%

Section: Samplingmentioning

confidence: 99%

Seasonal and spatial variability of dimethylsulfoniopropionate (DMSP) in the Mediterranean seagrass Posidonia oceanica

Borges

Champenois

2015

Aquatic Botany

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“…The algae were grown at 0 øC in 1-2 L beakers containing filtered North Sea water enriched with nutrients [Wiencke, 1990] For determination of DMSP concentrations in macroalgae, samples of incubated algae were treated and investigated as described by Karsten et al [ 1994]. The algal material was dried at 70 øC for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Methyl halides from Antarctic macroalgae

Laturnus¹,

Adams²,

Wiencke³

1998

Geophysical Research Letters

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“…However, a number of benthic marine organisms have also been shown to produce or contain DMSP. Early work identified macroalgae as an important source, with DMSP concentrations between 0 and 85 µmol g -1 in live seaweed (Reed 1983, Karsten et al 1994. Other benthic sources of DMSP, include tropical corals (Hill et al 1995, Broadbent et al 2002, coral mucus and mucus ropes (Broadbent & Jones 2004) and cord-grass (Dacey et al 1994).…”

Section: Introductionmentioning

confidence: 99%

“…While fleshy red algae are known to contain DMSP (e.g. Polysiphonia sp., 150 nmol g -1 fresh wt; Karsten et al 1994), in non maerl-forming red coralline algae (e.g. Corallina sp.)…”

Section: Introductionmentioning

confidence: 99%