A method for accurate determination of volume and cell numbers in small organs

Dornfeld, Ernst J.; Slater, Daniel; Scheffé, Henry

doi:10.1002/ar.1090820211

Cited by 74 publications

(9 citation statements)

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“…The number of oocytes was estimated by the method of Dornfeld, Slater & Scheffé [1942]. Every eleventh section was marked, and all oocytes with nucleoli were counted and classified in six groups, normal and atretic follicles being differentiated : Group 1 One layer of flattened or rounded granulosa cells Group 2 One layer of cuboidal granulosa cells Group 3 Two layers of cuboidal granulosa cells Group 4 Three layers of cuboidal granulosa cells Group 5 Four layers of cuboidal granulosa cells but antrum absent Group 6 Antrum present.…”

Section: Methodsmentioning

confidence: 99%

The Numbers of Normal and Atretic Ova in the Mature Rat

Mandl¹,

Zuckerman²

1950

Journal of Endocrinology

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Section: Methodsmentioning

confidence: 99%

The Numbers of Normal and Atretic Ova in the Mature Rat

Mandl¹,

Zuckerman²

1950

Journal of Endocrinology

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“…Follicles with a nucleolus in the oocyte were counted. The total number of follicles was estimated using the method of Dornfeld et al (1942). The diameter of the follicles was determined as the mean of the longest and shortest diameter of each follicle measured as a straight-line distance between opposite points of the basement membrane.…”

Section: Histological Proceduresmentioning

confidence: 99%

Protective effects of D-Trp6-luteinising hormone-releasing hormone microcapsules against cyclophosphamide-induced gonadotoxicity in female rats

Bokser¹,

Szende²,

Schally³

1990

Br J Cancer

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Summary The possible protective effect of an agonist of luteinising hormone-releasing hormone (LH-RH) against the ovarian damage caused by cyclophosphamide was investigated in rats. D-Trp6-LH-RH microcapsules were injected once a month for 3 months, in a dose calculated to release 25 jg day-. Control animals received the injection vehicle. Sixty days after the first injection of microcapsules, cyclophosphamide was given at a loading dose of 50mgkg-' followed by 5 mg kg-' day-' for 30 days, while the treatment with D-Trp6-LH-RH was continued. When the ovaries were examined 3 months and 5 months after discontinuation of treatment, a significant reduction in the total number of follicles (P<0.01) was found in non-pretreated animals given cyclophosphamide. This reduction affected mainly follicles larger than 100 .tm. An irreversible disintegration and destruction of granulosa cells was also observed in this group. In animals pretreated with D-Trp6-LH-RH, administration of cyclophosphamide caused no reduction in the number and diameter of follicles. Thus, the treatment with D-Trp6-LH-RH microcapsules before and during chemotherapy prevented the ovarian injury inflicted by cyclophosphamide. The suppression of gonadal function by LH-RH analogues could be possibly utilised for the protection of the ovaries against damage caused by cytotoxic drugs.

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“…Through the method of Chalkley, with the exception of Day 50 (one animal), 600 cells were scored as to germinal or somatic character in the largest sagittal section of one ovary of each of three animals at fourteen selected stages of gestation (Table 1). Likewise, the volume of at least one ovary of each of three foetuses at points up to Day 170 was calculated after the method of Dornfeld et al (1942).…”

Section: Histologymentioning

confidence: 99%

Development and Radio-Response of the Prenatal Bovine Ovary

Erickson¹

1966

Reproduction

163

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Qualitative observations of forty-nine bovine foetuses taken at varying stages of gestation revealed the following: (1) the germinal ridge is established at the 32nd day of gestation; (2) due to the formation of the tunica albuginea of the primitive testis, foetal sex is distinguishable by Day 39; (3) meiosis begins in ovaries at 75 to 80 days post coitum; (4) oogonial mitosis is discontinued by 150 to 170 days; (5) the majority of the oocytes have attained their stage of rest (pachytene) and are enveloped in primordial follicles by Day 170; and (6) vesicular follicles appear in ovaries at Day 250 of gestation.Total germ-cell numbers, based on volumetric determinations or direct counts, increased from a low of approximately 16,000 at Day 50 to a high of 2,700,000 at Day 110. From Day 110 numbers declined abruptly to 107,000 at Day 170 and the ovaries of four foetuses at 270 days of gestation (average length of gestation, 283 days) contained an average of 68,000 \m=+-\ 21,000 germ cells.Seventy prenatally irradiated (400 R, whole-body to dam) and twenty-five non-irradiated heifers were slaughtered at either 9 or 23 months of age. Microscopic examinations of their ovaries revealed that irradiation was apparently without effect during the interval of 17 to 59 days of gestation, and of questionable effect during the interval of 59 to 119 days of gestation. Irradiation administered during the 119-to 154-day interval, however, diminished germ-cell numbers by an average of 68%. From Day 154 to term, irradiation was again without effect. The mitotically active oogonium was probably the cell affected during the interval of 59 to 119 days of gestation and the drastic germinal decline during the 119-to 154-day interval was believed to be related to the progression of oocytes from zygotene to pachytene, a transition during which the oocyte under normal conditions is highly susceptible to death.

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A method for accurate determination of volume and cell numbers in small organs

Cited by 74 publications

References 0 publications

The Numbers of Normal and Atretic Ova in the Mature Rat

The Numbers of Normal and Atretic Ova in the Mature Rat

Protective effects of D-Trp6-luteinising hormone-releasing hormone microcapsules against cyclophosphamide-induced gonadotoxicity in female rats

Development and Radio-Response of the Prenatal Bovine Ovary

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