A metabonomics and lipidomics based network pharmacology study of qi-tonifying effects of honey-processed Astragalus on spleen qi deficiency rats

Huang, Li; Ye, Mingzhu; Wu, Jiacai; Liu, Wuping; Chen, Hong‐Yuan; Rui, Wen

doi:10.1016/j.jchromb.2020.122102

Cited by 27 publications

(13 citation statements)

References 32 publications

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“…2009 ; Huang et al. 2020 ). TCM theory also holds that the spleen in the will (meaning Zhi in TCM) is thinking, which is a state of human mental thinking and consciousness activities (Ouyang et al.…”

Section: Discussionmentioning

confidence: 99%

Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis

Zhang

et al. 2022

Pharmaceutical Biology

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Context Hedysari Radix Praeparata Cum Melle (HRPCM) and Astragali Radix Praeparata Cum Melle (ARPCM) are used interchangeably in clinics to treat spleen-qi deficiency (SQD) symptom mainly including gastrointestinal dysfunction and decreased immunity, which has unknown differences in efficacy. Objective To investigate the differences between HRPCM and ARPCM on intervening gastrointestinal- and immune-function with SQD syndrome. Materials and methods After the SQD model was established, the Sprague–Dawley (SD) rats were randomly divided into nine groups ( n = 10): normal; model; Bu-Zhong-Yi-Qi Pills; 18.9, 12.6 and 6.3 g/kg dose groups of HRPCM and ARPCM. Gastrointestinal function including d -xylose, gastrin, amylase vasoactive intestinal peptide, motilin, pepsin, H + /K + -ATPase, Na + /K + -ATPase, sodium-glucose cotransporter 1 (SGLT1), glucose transporter 2 (GLUT2) and immune function including spleen and thymus index, blood routine, interleukin (IL)-2, IL-6, interferon-γ (IFN-γ), tumour necrosis factor-α (TNF-α), immunoglobulin (Ig) M, IgA, IgG and delayed-type hypersensitivity (DTH) were detected. Finally, the efficacy differences were analysed comprehensively by the fuzzy matter-element method. Results In regulating immune, the doses differences in efficacy between HRPCM and ARPCM showed in the high-dose (18.9 g/kg), but there were no differences in the middle- and low- dose (12.6 and 6.37 g/kg); the efficacy differences were primarily reflected in levels of IL-6, IFN-γ, TNF-α and IgM in serum, and the mRNA expression of IL-6 and IFN-γ in the spleen. In regulating gastrointestinal, the efficacy differences were primarily reflected in the levels of D -xylose, MTL, and GAS in serum, and the mRNA and protein expression of SGLT1 and GLUT2 in jejunum and ileum. Discussion and conclusions HRPCM is more effective than ARPCM on regulating gastrointestinal function and immune function with SQD syndrome. Therefore, we propose that HRPCM should be mainly used to treat SQD syndrome in the future.

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Section: Discussionmentioning

confidence: 99%

Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis

Zhang

et al. 2022

Pharmaceutical Biology

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“…Therefore, the study of the chemical differences between the RAR and PAR may be helpful to reveal the material basis for the improved in tonic effects of PAR. The previous research ( Li et al, 2020 ) demonstrated that the Qi-tonifying effects of PAR were related to the isoflavonoids and cycloastragenols by mediating immune function. Our study found that malonyl isoflavonoids and cycloastragenols in RAR converted to corresponding acetyl isoflavonoids, cycloastragenols, and isoflavonoid glycosides during roasting, resulting in the high content of acetyl metabolites in PAR.…”

Section: Discussionmentioning

confidence: 99%

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

Huang

Sun

et al. 2022

Front. Chem.

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Astragali Radix (AR), which is extensively used as a healthy food supplement and medicinal herb, contains two forms of products corresponding to raw Astragalus Radix (RAR) and processed Astragali Radix (PAR), which was obtained by roasting. In this study, a non-targeted rapid resolution liquid chromatography coupled with quadruple time-of-flight mass spectrometry (RRLC-Q/TOF-MS) based metabolomics approach was developed to investigate the chemical changes of AR due to roasting. A total of 63 compounds were identified or tentatively identified. Among them, 23 isoflavonoids (composed of 12 isoflavones, eight pterocarpans, and three isoflavans) and six cycloastragenols were characterized as differential metabolites. Heatmap visualization and high-performance liquid chromatography coupled with photodiode array and evaporative light scattering detector (HPLC–PDA-ELSD) quantitative analysis revealed that malonyl isoflavonoids or cycloastragenols were at higher levels in RAR. These might be converted to corresponding acetyl isoflavonoids and cycloastragenols and related isoflavonoid glycosides during roasting. To prove this prediction, chemical conversion experiments on malonyl isoflavonoids and cycloastragenols were performed to confirm and clarify the chemical transformation mechanism.

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“…28 Currently, growing numbers of researchers have attempted to integrate network pharmacology with metabolomics or lipidomics to reveal the therapeutic roles of medicinal herbs on complicated pathogenesis. 29,30 Furthermore, molecular docking is an important approach to model the interactions between small-molecule ligands and receptor proteins at the atomic level, which allows visualization of their binding sites and patterns by calculating each binding energy. Thus, it seems to be a promising way to predict the core protein targets for active constituents, with the aim of explaining the molecular mechanism of natural medicines.…”

Section: Introductionmentioning

confidence: 99%

Tissue lipidomics, network pharmacology, and molecular docking to explore the therapeutic mechanism of anthocyanins from Lycium ruthenicum Murr. against gouty arthritis

Li¹,

Jia²,

Zhang³

et al. 2023

Food Funct.

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A metabonomics and lipidomics based network pharmacology study of qi-tonifying effects of honey-processed Astragalus on spleen qi deficiency rats

Cited by 27 publications

References 32 publications

Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis

Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Praeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis

RRLC-QTOF/MS-Based Metabolomics Reveal the Mechanism of Chemical Variations and Transformations of Astragali Radix as a Result of the Roasting Process

Tissue lipidomics, network pharmacology, and molecular docking to explore the therapeutic mechanism of anthocyanins from Lycium ruthenicum Murr. against gouty arthritis

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