A metabolomics research based on UHPLC‐ESI‐Q‐TOF‐MS coupled with metabolic pathway analysis: Treatment effects of stir‐frying Xanthii Fructus on allergic rhinitis in mice model

Abstract

Xanthii Fructus (XF), a well-known herb in traditional Chinese medicine, has been frequently used for the treatment of allergic rhinitis in the clinic. Its therapeutic metabolic mechanism, however, remains undetermined. In this work, a metabolomics research coupled with metabolic pathway analysis has been employed to screen out the potential mechanism in its effects on allergic rhinitis. Specifically, mouse serum samples containing XF were analyzed based on ultra-high performance liquid chromatography equipped with electrospray ionization quadruple time-of-flight mass spectrometry detection (UHPLC-ESI-Q-TOF-MS) in both positive and negative polarity. In addition, the raw data gained from UHPLC-ESI-Q-TOF-MS were processed by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) in order to discover remarkable metabolites. Twenty-seven potential biomarkers in mouse serum were filtered from free databases like HMDB. Interestingly, this study filtered the potential metabolic pathways including glycerophospholipid metabolism and branch-chain amino acid metabolism. We hope that this paper will provide a feasible strategy for revealing the therapeutic mechanism of XF in allergic rhinitis mice model.

The Natural Products Targeting on Allergic Rhinitis: From Traditional Medicine to Modern Drug Discovery

“…Xanthii Fructus, the ripe fruits of Xanthium sibiricum Patr., is a well-known herb used as traditional Chinese medicine [105]. OVA-sensitized Male BALB/C mice were orally treated with 1 g/kg of stir-fried Xanthii Fructus ethanol extract for 15 days.…”

Metabolomics biotechnology, applications, and future trends: a systematic review

“…Twenty-seven potential biomarkers were discovered, mainly involved in metabolic pathways such as glycerophospholipid metabolism and branched-chain amino acid metabolism. 61 Wang, Y. et al found that gross saponins of Tribulus terrestris fruit can reverse middle cerebral artery occlusion-induced serum metabolic deviations by regulating multiple metabolic pathways. 62 Zhou, J. et al found that gastrointestinal injury was mainly related to vitamin B6 metabolism, phenylalanine metabolism, arachidonic acid metabolism, and taurine.…”

Comparative analysis of the liver‐protective effects of raw and stir‐fried semen of Hovenia dulcis in rats via gas chromatography–mass spectrometry–based serum metabolomic profiling and chemometrics

“…Traditional Chinese medicines (TCMs) are highly processed herbs. Many processing methods have been developed for processing herbs, such as stir‐frying (Zhuang et al, 2018), steaming (Zhu et al, 2016), wine‐sautéing (Huang et al, 2014), salt‐processing (Wu et al, 2022), among others. These methods are designed to meet certain clinical requirements.…”