A metabolomic study on the biological effects of metal pollutions in oysters Crassostrea sikamea

Abstract

a b s t r a c t a r t i c l e i n f oMetal pollution has become a great threat to organisms in the estuaries in South China. In the present study, the oysters Crassostrea sikamea were collected from one clean (Jiuzhen) and five metal polluted sites (Baijiao, Fugong, Gongqian, Jinshan and Songyu). The tissue metal concentrations in oysters indicated that the five metal sites were polluted by several metals, including Cr, Ni, Co, Cu, Zn, Ag, Cd and Pb with different patterns. Especially, Cu and Zn were the major contaminants in Baijiao, Fugong and Jinshan sites. The metabolic responses in oysters C. sikamea indicated that the metal pollutions in BJ, FG, JS and SY sites induced disturbances in osmotic regulation and energy metabolism via different metabolic pathways. However, the metal pollution in GQ site mainly influenced the osmotic regulation in the oysters C. sikamea. This study demonstrates that NMR-based metabolomics is useful to characterize metabolic responses induced by metal pollution.

The Zebra Mussel (Dreissena polymorpha) as a Model Organism for Ecotoxicological Studies: A Prior 1H NMR Spectrum Interpretation of a Whole Body Extract for Metabolism Monitoring

“…in ecotoxicological studies is mainly limited by the lack of genomic data on most of the environmentally relevant species [17,18]. Metabolomics has been involved in several studies related to the impact of contaminants on numerous aquatic organisms [20], including marine bivalve species in laboratories (e.g., [21][22][23]) or in the field (e.g., [4,[24][25][26][27]).…”

“…However, whatever the employed analytical method, signal assignment remains one of the main challenges of metabolomics [17,29,30]. In most NMR-based ecotoxicological studies, spectral peak assignment is solely based on chemical shift values [22][23][24][25][26][27], and only rarely relies on additional experimental data like heteronuclear 1 H- 13 C chemical shift correlations. However, chemical shift values may vary according to the sample pH, to the presence of other metabolites in the sample, and of course, the internal standard used for spectrum referencing (most of the time, either sodium trimethylsilylpropanesulfonate or sodium 3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionate).…”

Proteomic responses induced by metal pollutions in oysters Crassostrea sikamea

“…Cytochrome c oxidase is an important cellular enzyme with a central role in oxidative metabolism (Stiburek et al, 2006) and the activity of cytochrome c oxidase can be used as an indicator of mitochondrial quantity and quality (Čapková et al, 2002). Cu is the main metal pollutant in the JS site (Ji et al, 2016). As reported, Cu can infl uence mitochondrial function by inducing oxidative stress and reducing the amount of energy from oxidative phosphorylation (Krumschnabel et al, 2005).…”

“…hongkongensis sampled in Baijiao and Fugong sites severely contaminated by Cu (Xu et al, 2016). Besides BJ site, JS site was also heavily polluted by Cu, indicated by as high as 6 746.7 μg/g Cu (dry weight) concentrated in oysters (Ji et al, 2016). Therefore, the 78 kDa glucose regulated protein could be a protein biomarker of Cu pollution in oysters.…”

Assessment of the Environmental Significance of Heavy Metals Pollution in Surficial Sediments of South Brittany Waters, France: An Ecological Risk Assessment Approach

“…It occurred in numerous residents of the Jinzu River basin in Toyama Prefecture, Japan, a region extremely polluted with Cd that originated from a zinc mine located upstream (Baba et al, 2013;Ogawa et al, 2004). Moreover, green-colored oysters as a result of Cu contamination have been found in several estuaries and harbors around the world, including USA (Roosenburg, 1969), Spain (Funes et al, 2006), China (Ji et al, 2016;Wang et al, 2011), and Taiwan (Fang and Dai, 2017;Lee et al, 1996). Chronic Cu toxicity can cause liver disease and critical neurological damage (Ali and Khan, 2019;Gao et al, 2013).…”