A metabolomic study on early detection of steroid-induced avascular necrosis of the femoral head

Ren, Xiangnan; Wu, Fan; Shao, Zixing; Chen, Kaiyun; Yu, Xuefeng; Liang, Qionglin

doi:10.18632/oncotarget.24150

Cited by 21 publications

(30 citation statements)

References 44 publications

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“…According to the related reference [35], the samples were taken at the speci c nodes and serum samples were prepared. Above 3 mL venous blood was gotten from speci c rabbits.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

“…The indexes were as follows: 3D T 2 WI VISTA (TR 2600 ms, TE 200 ms), slice thickness 1.2 mm, ip angle 90 deg and matrix (reconstruction 256 × matrix scan 256). Then the MRI results of lovastatin for treatment of SANFH were researched [35].…”

Section: Magnetic Resonance Imaging Scanmentioning

confidence: 99%

“…Acquity™ UPLC BEH C18 column (2.1 × 100 mm, 1.7 µm) (Waters, USA) was used. The chromatographic condition in reference was used [35] . The mobile phase consisting of isopropanol/facetonitrile 50:50 (V/V) with 0.1% formic acid (mobile phase A) and 0.1% formic acid solution (mobile phase B).…”

Section: Uhplc-ms/ms Analysismentioning

confidence: 99%

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Untargeted metabolomics reveals the effect of lovastatin on steroid-induced necrosis of the femoral head in rabbits

Ren

Shao

et al. 2020

Preprint

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PURPOSE Lovastatin is an important medicine and it shows a significant effect against glucocorticoid-induced necrosis of the femoral head. This study aimed to investigate the effect of lovastatin on preventing necrosis of the femoral head of by serum metabolomics strategy. METHODS Adult healthy adult Japanese white rabbits were divided into three groups: control group, model group and drug group. The pathologic changes of femoral head were assessed with magnetic resonance imaging and microscope. Metabolomics based on UHPLC-MS/MS was used to analyze the collected serum sample. Data were analyzed using principal component analysis (PCA), partial least squares-discriminate analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). All potential metabolites were identified by comparing with HMDB database, metlin database, lipid maps and chemspider database. RESULTS 11 potential biomarkers were noted and identified as potential biomarkers. The change of biomarkers suggested that lovastatin on preventing necrosis of the femoral head may affect glycerophospholipid metabolism, linoleic acid metabolism, sphingolipid metabolism, alpha-Linolenic acid metabolism, pyrimidine metabolism, arachidonic acid metabolism. CONCLUSION The study suggested that lovastatin could prevent the glucocorticoid-induced necrosis of the femoral head of rabbits. The possible reasons were closely associated with adjusting the lipid metabolism, inhibiting adipogenesis and delaying the osteocyte apoptosis.

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“…According to the related reference [35], the samples were taken at the speci c nodes and serum samples were prepared. Above 3 mL venous blood was gotten from speci c rabbits.…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

Section: Magnetic Resonance Imaging Scanmentioning

confidence: 99%

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Untargeted metabolomics reveals the effect of lovastatin on steroid-induced necrosis of the femoral head in rabbits

Ren

Shao

et al. 2020

Preprint

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“…However, the pathogenesis of steroid-induced ONFH is still not very clear. Presently, osteoblast apoptosis, 10,11 lipid metabolism abnormality, 12 intravascular coagulation, 13 and vascular endothelial dysfunction 14 are hypothesized as factors causing ONFH. However, a study indicated that the pathogenesis of steroid-induced ONFH was the result of a comprehensive effect of multiple factors.…”

Section: Introductionmentioning

confidence: 99%

Effect of glucocorticoids on the function of microvascular endothelial cells in the human femoral head bone

Lu¹,

Yu²,

Guo³

et al. 2020

Adv Clin Exp Med

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Background. The pathogenesis of glucocorticoid (GC)-induced osteonecrosis (ON) of the femoral head remains unclear. Recent research has suggested that it is closely associated with injured bone microvascular endothelial cells (BMECs). However, few studies have used BMECs to perform research pertaining ON of the femoral head. Objectives. The objective of this study was to investigate the functional changes of BMECs treated with a GC and to detect the changes in related genes using microarrays. Material and methods. Cells were isolated using an enzymatic method and identified with EC markers, such as von Willebrand factor (vWF), CD31 and vascular endothelial cadherin (VE-cadherin). Bone microvascular endothelial cells were treated with 0. 1 mg/mL and 0.3 mg/mL of hydrocortisone to establish a GCdamaged model of BMECs. The mRNA microarrays were used to detect the differential expression profiles between BMECs with and without GC damage. Results. Primary cells appeared as having a cobblestone-like morphology. Immunofluorescence staining revealed that the cells were 100% positive for vWF and CD31, and near 100% positive for VE-cadherin. It also confirmed that the cells were BMECs. Bone microvascular endothelial cells treated with 0. 1 mg/mL of hydrocortisone showed shrinkage, and those treated with 0.3 mg/mL of hydrocortisone mostly showed apoptosis. The mRNA microarray showed that genes associated with endothelial cells, such as endothelin 1 (ET-1) receptor, angiotensin II (AII) receptor, intercellular adhesion molecule 1 (ICAM-1), and plasminogen activator inhibitor 1 (PAI-1), were upregulated, and genes associated with endothelial nitric oxide synthase (eNOS), endothelin 1 (ET-1), prostaglandin I 2 (PGI 2) synthase, PGI 2 receptor, vascular endothelial growth factor (VEGF), prostaglandin E (PGE) synthase, and PGE receptor were downregulated. The results of quantitative polymerase chain reaction (qPCR) validation were consistent with the findings of mRNA microarrays. Conclusions. Glucocorticoids promoted BMECs to express vasoconstrictors and procoagulant factors and related receptors, and decreased the expression of vasodilators and their receptors.

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“…Avascular necrosis is an adverse outcome of femoral neck fractures, and adequate reduction and internal fixation may reduce avascular necrosis after osteosynthesis. The femoral head avascular necrosis rate in the elderly can be decreased through closed reduction and fixation, and reduction quality, fracture type, and the timing of the operation are closely correlated with femoral head avascular necrosis. Several studies have explored the risk differences of avascular necrosis in femoral neck fractures in children and adolescents who have received open reduction or closed reduction.…”

Section: Introductionmentioning

confidence: 99%

Poor Outcomes of Children and Adolescents with Femoral Neck Fractures: A Meta‐Analysis Based on Clinical Studies

Chen

Zhang²,

Guo

et al. 2020

Orthopaedic Surgery

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Objective: To comprehensively assess the differences in outcome between open reduction and closed reduction for children and adolescents with femoral neck fractures.Methods: Based on the predetermined strategies, eligible studies were obtained by searching Embase, the Cochrane Library, and PubMed databases (retrieval time: June 2018) and through manual retrieval for paper documents. The 95% confidence intervals (CI) and risk ratios (RR) were used as evaluation indexes. Moreover, the results of avascular necrosis, coxa vara, or non-union were compared between open reduction and closed reduction under random or fixed effects models. After sensitivity analysis was carried out, publication bias was evaluated for the eligible studies using Egger's test.Results: Six studies were included in our meta-analysis. No significant heterogeneity was found among the included studies (P ≥ 0.05) and, thus, the fixed effects model was used for merging the effect sizes of avascular necrosis (RR [95% CI] = 0.50 [0.26, 0.98], P = 0.04), coxa vara (RR [95% CI] = 0.16 [0.04, 0.70], P = 0.01), and non-union (RR [95% CI] = 0.22 [0.05, 0.93], P = 0.04). Sensitivity analysis suggested that the results of avascular necrosis were not stable (RR = 0.50, 95% CI = 0.25 1.17, P = 0.12), while those of coxa vara and non-union were stable. There was no significant publication bias among the eligible studies (t = −0.70, P = 0.522).Conclusion: Femoral neck fractures treated by open reduction had less adverse outcomes compared with those treated by closed reduction.

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A metabolomic study on early detection of steroid-induced avascular necrosis of the femoral head

Cited by 21 publications

References 44 publications

Untargeted metabolomics reveals the effect of lovastatin on steroid-induced necrosis of the femoral head in rabbits

Untargeted metabolomics reveals the effect of lovastatin on steroid-induced necrosis of the femoral head in rabbits

Effect of glucocorticoids on the function of microvascular endothelial cells in the human femoral head bone

Poor Outcomes of Children and Adolescents with Femoral Neck Fractures: A Meta‐Analysis Based on Clinical Studies

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