A Meta-Analysis on Human Papillomavirus Type Distribution among Women with Cervical Neoplasia in the WHO Eastern Mediterranean Region

Shoja, Zabihollah; Farahmand, Mohammad; Hosseini, Neda; Jalilvand, Somayeh

doi:10.1159/000502824

Cited by 16 publications

(19 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPV genotyping indicated that CINs were mainly positive for HPV16, followed by HPV18. Our data are in agreement with previous investigations indicating that HPV16 was the most common genotype in uterine cervical precancerous lesions, followed by HPV 18 (Shoja et al, 2019). However, other studies reported additional HPVs, including HPV 6/11/31/33/45, although at lower prevalence (Ogembo et al, 2015;Shoja et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

et al. 2020

View full text Add to dashboard Cite

Human papillomaviruses (HPVs) are small DNA tumor viruses that mainly infect mucosal epithelia of anogenital and upper respiratory tracts. There has been progressive demand for more analytical assays for HPV DNA quantification. A novel droplet digital PCR (ddPCR) method was developed to simultaneously detect and quantify HPV DNA from different HPV types. DdPCR was initially tested for assay sensitivity, accuracy, specificity as well as intra-and inter-run assay variation employing four recombinant plasmids containing HPV16, HPV18, HPV11, and HPV45 DNAs. The assay was extended to investigate/quantify HPV DNA in Cervical Intraepithelial Neoplasia (CIN, n = 45) specimens and human cell lines (n = 4). DdPCR and qPCR data from clinical samples were compared. The assay showed high accuracy, sensitivity and specificity, with low intra-/inter-run variations, in detecting/quantifying HPV16/18/11/45 DNAs. HPV DNA was detected in 51.1% (23/45) CIN DNA samples by ddPCR, whereas 40% (18/45) CIN tested HPV-positive by qPCR. Five CIN, tested positive by ddPCR, were found to be negative by qPCR. In CIN specimens, the mean HPV DNA loads determined by ddPCR were 3.81 copy/cell (range 0.002-51.02 copy/cell), whereas 8.04 copy/cell (range 0.003-78.73 copy/cell) by qPCR. DdPCR and qPCR concordantly detected HPV DNA in SiHa, CaSki and Hela cells, whereas HaCaT tested HPV-negative. The correlation between HPV DNA loads simultaneously detected by ddPCR/qPCR in CINs/cell lines was good (R 2 = 0.9706, p < 0.0001). Our data indicate that ddPCR is a valuable technique in quantifying HPV DNA load in CIN specimens and human cell lines, thereby improving clinical applications, such as patient management after primary diagnosis of HPV-related lesions with HPV-type specific assays.

show abstract

Section: Discussionsupporting

confidence: 93%

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The most prevalent HPV in this study was genotype 16 at 42.9%, which is in line with the findings of most studies conducted in the region (28,31,33,34), and other areas of the world where the HPV vaccine was not implemented effectively (32,35). Other results in this study might deviate from the findings reported elsewhere due to a difference in the age group compared to other studies.…”

Section: Discussionsupporting

confidence: 87%

Prevalence and Genotype Distribution of Human Papillomavirus Among a Subpopulation of Jordanian Women

Khasawneh¹,

Asali²,

Kilani³

et al. 2020

International Journal of Women’s Health and Reproduction Scienc

View full text Add to dashboard Cite

Objectives: Human papillomavirus (HPV) infection is the leading sexually-transmitted infection (STI) worldwide and the main etiology of cervical cancer. HPV infection rates are important in directing vaccination policies and screening for cervical cancer. Unfortunately, no recent reports have evaluated the prevalence of this infection among Jordanian women despite major globalization-driven changes in sexual behavior. Accordingly, this study aimed to determine the prevalence of HPV infection and its genotypic distribution in the cervical samples of Jordanian women. Materials and Methods: The study was carried out at Prince Hamza Hospital (Amman, Jordan) during 2016-2017. Women (15-75 years old) were randomly selected for cervical cell collection. DNA was extracted and then amplified using MY09/11 and GP5+/6+ consensus primers. Finally, positive samples were genotyped by applying real-time-polymerase chain reaction and reverse line blotting. Results: Fourteen out of 348 women tested positive for HPV with a prevalence rate of 4%. In addition, multiple HPV genotypes were observed in 36% (5/14) of infected women while single HPV genotype infection was found in 64% (9/14) of infected women. Further, high-risk (HR), potential high-risk (pHR), and low-risk (LR) HPV genotypes were detected in most cases with a 78.6% (11/14) infection rate, 42.9% (6/14), and 7.1% (1/14) of infected women, respectively. Eventually, 10 different genotypes were detected in infected women and HPV 16 was the most common type (42.9%, 6/14). Conclusions: Our data suggest that the prevalence of HPV infection among Jordanian women is below the global and regional rates. It is hoped that these data should facilitate the implementation of appropriate cervical cancer screening and future HPV vaccination programs.

show abstract

“…The infection rate during these ages was significantly higher compared to other age groups, suggesting a “U”-shaped infection. Our results are not in agreement with previously studies where authors reported that the highest peak was obtained for younger women (under 25 years old), then a decreasing trend with age was observed and another maximum peak around 50 years old [ 15 , 19 – 23 ]. Many variables including geographic locations and population, demographic factors and possibly vaccination could be responsible for this difference in the prevalence of HPV-positive status and age trend modification.…”

Section: Discussioncontrasting

confidence: 99%

“…We can conclude that despite the small size of our subgroup, our results are in agreement with several reports. Our data confirm the correlation between HPV infection prevalence and the gravity of cervical intraepithelial neoplasia subgroup [ 23 , 28 , 29 ].…”

Section: Discussionsupporting

confidence: 89%

Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women

Rhouma

Ardhaoui

Fehri

et al. 2021

Infect Agents Cancer

View full text Add to dashboard Cite

Background High-risk human papillomavirus (HR-HPV) are responsible for cervical cancer (CC) which represents the second most prevalent gynecological cancer among Tunisian women. Preventive strategies against CC are based on prophylactic vaccines that have not yet been implemented into the national vaccination program of Tunisia. Therefore, the present study aimed to investigate the HPV genotypes distribution in cervical neoplasia in Tunisian women in order to predict the impact of using current HPV vaccines on cancer prevention in Tunisia. Methods A total of 200 formalin-fixed paraffin embedded biopsies were collected in our study. DNA was extracted using Qiagen Mini prep kit. DNA quality was controlled by Beta Globin PCR. Only positive samples for Beta Globin test were used. HPV detection was performed by a nested PCR using PYGMY and GP5+/6+ primers. Genotyping was performed by Reverse Line hybridization using 31 probes. Results The mean age of participants was 38.97 years and 75% were over 30 years. Cervical neoplasia distribution according to age showed that CINII/CINIII was observed among women over 30 years old. All samples were positive for Beta Globin PCR. Overall HPV prevalence in cervical lesions was 83% (166/200). HPV was present in 65% of CINI, 82% of CINII/CINIII and 85% of CC. HR-HPV was statistically significantly associated with cervical intraepithelial neoplasia (p < 10–3). HR-HPV distribution according to lesion grade and cervical cancer showed that HPV16 and HPV18 were present in all lesions. For CINII/CINIII, HPV 35 (37.5%) was the most detected type, followed by HPV18 (33.3%) HPV 45 (28.5%) and HPV 16 (18.9%). HPV 45(57.5%), HPV 18 (53.3%) were the most detected in CC. HPV58, 59, 68 were only detected in CC and associated with HPV45, 18 and HPV16. HPV39, 31, 33, 52, 56 and HPV70 was associated only with CINI. Conclusions Our findings can give useful information for vaccine implementation by helping the health policymakers to choose the most appropriate vaccine type in Tunisia.

show abstract

A Meta-Analysis on Human Papillomavirus Type Distribution among Women with Cervical Neoplasia in the WHO Eastern Mediterranean Region

Cited by 16 publications

References 74 publications

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Simultaneous Detection and Viral DNA Load Quantification of Different Human Papillomavirus Types in Clinical Specimens by the High Analytical Droplet Digital PCR Method

Prevalence and Genotype Distribution of Human Papillomavirus Among a Subpopulation of Jordanian Women

Distribution of human papillomavirus in precancerous and cancerous cervical neoplasia in Tunisian women

Contact Info

Product

Resources

About