A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis

Long, J. Bradford De; Moan, Erich; Medford, June I.; Barton, Matt

doi:10.1038/379066a0

Cited by 1,422 publications

(1,253 citation statements)

References 18 publications

Supporting

Mentioning

1,182

Contrasting

Unclassified

Order By: Relevance

“…1998). ZWILLE has been suggested to specify the expression pattern of SHOOTMERISTEMLESS (STM), which is also involved in the maintenance of the apical pole (Long et al 1996). Our RT-PCR data indicate that expression of an STM homolog increased in BSO-treated embryos at stages 3.…”

Section: Developmental Responsementioning

confidence: 57%

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Stasolla

Belmonte

Tahir

et al. 2008

Planta

View full text Add to dashboard Cite

Applications of buthionine sulfoximine (BSO), an inhibitor of GSH (reduced glutathione), which switches the cellular glutathione pool towards the oxidized form GSSG, positively inXuences embryo quality by improving the structure of the shoot apical meristem and promoting embryo maturation, both of which improve the post-embryonic performance of the embryos. To investigate the mechanisms underlying BSO-mediated improvement in embryo quality the transcript proWles of developing Brassica napus microspore-derived embryos cultured in the absence (control) or presence of BSO were analyzed using a 15,000-element B. napus oligo microarray. BSO applications induced major changes in transcript accumulation patterns, especially during the late phases of embryogenesis. BSO aVected the transcription and activities of key enzymes involved in ascorbate metabolism, which resulted in major Xuctuations in cellular ascorbate levels. These changes were related to morphological characteristics of the embryos and their postembryonic performance. BSO applications also activated many genes controlling meristem formation and function, including ZWILLE, SHOOTMERISTEMLESS, and ARG-ONAUTE 1. Increased expression of these genes may contribute to the improved structural quality of the shoot poles observed in the presence of BSO. Compared to their control counterparts, middle-and late-stage BSO-treated embryos also showed increased accumulation of transcripts associated with the maturation phase of zygotic embryo development, including genes encoding ABA-responsive proteins and storage-and late-embryogenic abundant (LEA) proteins. Overall these transcriptional changes support the observation that the BSO-induced oxidized glutathione redox state allows cultured embryos to reach both morphological and physiological maturity, which in turn guarantees successful regeneration and enhanced post-embryonic growth.

show abstract

Section: Developmental Responsementioning

confidence: 57%

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Stasolla

Belmonte

Tahir

et al. 2008

Planta

View full text Add to dashboard Cite

show abstract

“…As STM, a class I KNOTTED-like protein, is expressed specifically in the shoot apical meristem (Long et al, 1996), we reasoned that the expression value measured for STM in the root could be used as a base line for gene expression in this tissue (Supplementary Table S3). Only AGL31 showed lower values than those for STM in the endodermis+cortex.…”

Section: Identified Ncatfs Are Expressed In Root Tissuesmentioning

confidence: 99%

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

Rim

Huang

Chu

et al. 2011

Molecules and Cells

View full text Add to dashboard Cite

In plants, cell-to-cell communication is pivotal for the orchestration of cell fate determination, organ development, and the integration of whole plant physiology. One of the strategies for intercellular communication uses symplasmic communication channels, called plasmodesmata (PD). These PD establish unique cytoplasmic channels for the intercellular exchange not only of metabolites and small signaling molecules, but also of regulatory proteins and RNAs to allow for local orchestration of development and physiology. A number of non-cell-autonomous transcription factors (NCATFs) have been shown to function in the coordination of specific regulatory networks. To further explore the potential of such NCATFs, a genome-wide screen was performed on the transcription factor (TF) families in Arabidopsis. We here report that, among the 76 TFs examined, 22 were shown to move beyond their sites of transcription in the root apex; these NCATFs belonged to 17 TF families, including homeobox, GRAS, and MYB. Expression studies performed on variously-sized mCherry constructs identified a range of PD size exclusion limits within tissues of the root. In addition, our studies showed that actual protein level was an important factor controlling the range of TF intercellular movement. Interestingly, our studies on CAPRICE movement revealed tissue-specificity with respect to the mode of intercellular trafficking. These findings are discussed with respect to the regulation between cell-autonomous or non-cell-autonomous action.

show abstract

“…These include the class I knotted-like homeobox (KNOX ) genes, which have been implicated as regulators of SAM formation and maintenance in both monocots and dicots (Reiser et al, 2000). In Arabidopsis, class I is comprised of four genes: SHOOT MERISTEMLESS (STM ), knotted-like from Arabidopsis thaliana (KNAT )1, KNAT2, and KNAT6 (Lincoln et al, 1994;Long et al, 1996;Semiarti et al, 2001). The STM gene is particularly important as mutations in the STM gene result in the lack of SAM formation (Barton and Poethig, 1993).…”

Section: Introductionmentioning

confidence: 99%

“…The STM gene is particularly important as mutations in the STM gene result in the lack of SAM formation (Barton and Poethig, 1993). STM is expressed throughout the SAM but is downregulated in the presumptive organ primordia (Long et al, 1996). In addition, transgenic plants overexpressing STM were severely stunted with a disorganized shoot meristem that produces many leaf-like structures that do not develop into mature leaves (Williams, 1998).…”

Section: Introductionmentioning

confidence: 99%

CUC1 gene activates the expression of SAM‐related genes to induce adventitious shoot formation

Hibara

Takada

Tasaka³

2003

The Plant Journal

177

138

View full text Add to dashboard Cite

SummaryCUP-SHAPED COTYLEDON (CUC )1 encodes members of the NAC family. These are functionally redundant genes that are involved in shoot apical meristem (SAM) formation and cotyledon separation during embryogenesis in Arabidopsis. We analyzed transgenic plants overexpressing CUC1 (35S::CUC1 ). The cotyledons of these transgenic seedlings regularly had two basal lobes, small and round epidermal cells between the sinuses, and adventitious SAMs on the adaxial surface of this region. This suggests that CUC1 promotes adventitious SAM formation by maintaining epidermal cells in an undifferentiated state. In 35S::CUC1 cotyledons, the class I knotted-like homeobox (KNOX ) genes, including SHOOT MERISTEMLESS (STM ) and BREVIPEDICELLUS (BP ), which are involved in SAM formation and/or maintenance, were ectopically expressed before adventitious SAM formation. In stm mutants, ectopic expression of CUC1 could not induce adventitious SAMs, whereas they continued to be observed in bp mutants. These results suggest that STM, but not BP, is necessary for the formation of adventitious SAMs in 35S::CUC1 cotyledons. Furthermore, we examined the relationship between CUC1 and ASYMMETRIC LEAVES (AS )1 and AS2. The as1 and as2 mutations genetically enhance 35S::CUC1 phenotypes even in the absence of STM function. Interestingly, the as1 mutation can partially rescue the mutant vegetative development phenotypes in the cuc1 cuc2 double mutant. Our results suggest that CUC1 positively regulates SAM formation not only through STM but also through an STM-independent pathway that is negatively regulated by AS1 and AS2.

show abstract

A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis

Cited by 1,422 publications

References 18 publications

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Buthionine sulfoximine (BSO)-mediated improvement in cultured embryo quality in vitro entails changes in ascorbate metabolism, meristem development and embryo maturation

Analysis of Arabidopsis Transcription Factor Families Revealed Extensive Capacity for Cell-to-Cell Movement as Well as Discrete Trafficking Patterns

CUC1 gene activates the expression of SAM‐related genes to induce adventitious shoot formation

Contact Info

Product

Resources

About