A Medium for, Initial Visual Demonstration of Production of Coagulase and Fermentation of Mannitol by Pathogenic Staphylococci

The potential hazard of staphylococci and micrococci to human subjects in the life support systems evaluator with elevated cabin temperature

“…This was shown to be the case when a l l the coagulase-positive cultures (plate method) were tested by the tube method of Fisk (33). Statistical analysis was carried out on C types as determined by the tube method (33) and by the plate method (28). The results of t h i s analysis appear i n table VI1 which indicates statistical agreement between both methods i n the frequency of occurrence o f C types under body areas "A".…”

“…Underlined number refer to those types found to be significantly higher when their averages were compared by the Duncan Multiple Range Test (35). The nose, groin, glans penis, and anus exhibited the largest frequency of C M types as determined by the plate method of Esber and Faulconer (28); the nose, the largest frequency of C types as determined by the tube method of Fisk (33); D types occurred most frequently i n the nose, groin, glans penis, anus, and toes. Of a l l body areas "A" listed, the nose, groin, glans penis, and anus predominate as those areas most likely to harbor potentially pathogenic staphylococci.…”

“…In the present study coagulase production as determined by the modified tube method of Fisk (33) was selected as the main index of staphylococcal potential pathogenicity, because Lotter and Horstman found (32) that about one-tenth a5 many coagulase-positive cultures are detected by the tube method of Fisk (33) as by the plate method of Esber and Faulconer (28). However, the factor(s) i n the plate medium responsible for the discrepancy between the two meihods have not been identified.…”

“…Before replication to biochemical test media, staphylococci were grown on 5% sheep blood agar plates and theri colonies showed hemolysis after 48 hours of aerobic incubation a t 37°C. Coagulase production and mannitol utilization were observed on the coagulase-mannitol plate medium of Esber and Faulconer (28), to which 15% sterile coagulase horse plasma (B. B. L .)…”

“…plates (31). Lotter and Horstrnan (32) found that results of the coagulase plate method (28) are unreliable after a l l coagulase-positive cultures had been tested by the coagulase tube method (33).…”