A Mediator‐Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode

Yao, Yan; Shiu, Kwok Keung

doi:10.1002/elan.200804297

Cited by 40 publications

(18 citation statements)

References 37 publications

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“…There have also been combinations of GOx and horse radish peroxidase (HRP) coupled to nanotubes and the electrode through a nafion polymer film [131]. In such systems, the HRP directly undergoes electron transfer with the electrode to indirectly measure the glucose concentration through hydrogen peroxide detection [131].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

“…In such systems, the HRP directly undergoes electron transfer with the electrode to indirectly measure the glucose concentration through hydrogen peroxide detection [131]. More recently, a more stable and sensitive biosensors have been developed for glucose using a chitosan-bovine serum albumen cryogel incorporated with MWCNTs, GOx, and ferrocene [132].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

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Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

187

111

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a b s t r a c tSince the discovery of low-dimensional carbon allotropes, there is increasing interest in using carbon nanomaterials for biomedical applications. Carbon nanomaterials have been utilized in the biomedical field for bioimaging, chemical sensing, targeting, delivery, therapeutics, catalysis, and energy harvesting. Each application requires tailored surface functionalization in order to take advantage of a desired property of the nanoparticles. Herein, we review the surface immobilization of bio-molecules, including proteins, peptides, and enzymes, and present the recent advances in synthesis and applications of these conjugates. The carbon scaffold and the biological moiety form a complex interface which presents a challenge for achieving efficient and robust binding while preserving biological activity. Moreover, some applications require the utilization of the protein-nanocarbon system in a complex environment that may hinder its performance or activity. We analyze different strategies to overcome these challenges when using carbon nanomaterials as protein carriers, explore various immobilization techniques along with characterization methods, and present recent demonstrations of employing these systems for biomedical applications. Finally, we consider the challenges and future directions of this field.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Protein functionalized carbon nanomaterials for biomedical applications

Oliveira

Bisker

Bakh

et al. 2015

Carbon

187

111

View full text Add to dashboard Cite

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“…A good linear relationship was obtained in the concentration range of glucose from 10 mM to 400 mM and a correlation coefficient of 0.997 in 10 mM HQ. The sensitivity of the biosensor was 13.2 mA mM À1 cm À2 if the surface area of the GCE (0.07 cm 2 ) was used as the sensing area [38,39]. The detection limit of this biosensor was estimated as 2.3 mM at a signal-to-noise ratio of 3.…”

Section: Amperometric Response and Calibration Curvementioning

confidence: 99%

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite

Wang

Zhang

et al. 2010

Electroanalysis

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An exploration of gold nanoparticles-bacterial cellulose nanofibers (Au-BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au-BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au-BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 mM with a linear range from 10 mM to 400 mM. The biosensor was successfully applied to the determination of glucose in human blood samples.

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“…Accordingly, the fundamental principles for an electrochemical biosensor involve providing a suitable microenvironment for the enzyme to retaining its stability and bioactivity, and accelerating the electron transfer between enzyme and electrode to realize the direct electrochemistry of enzyme. For achieving these goals, various materials have been utilized to modify the surface of electrode and immobilize HRP, including conducting polymers [10,11], ionic liquid [12,13], biomolecule films [14,15], and inorganic nanostructured materials [16][17][18][19][20][21]. Application of nanomaterials in biosensors has attracted great interest in the past decades.…”

Section: Introductionmentioning

confidence: 99%

Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites

et al. 2011

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Graphene-Fe 3 O 4 (G-Fe 3 O 4 ) composites were prepared with a solvothermal method for the immobilization of horseradish peroxidase (HRP) to construct a mediator-free H 2 O 2 biosensor. The morphology and structure of the GFe 3 O 4 composites were analyzed by TEM, SEM, and XRD. Chitosan (CH) was utilized to disperse the composites, and the mixture of CH, G-Fe 3 O 4 and HRP was spin-coated on an indium tin oxide (ITO) electrode to form a biocomposites film. Electrochemical impedance spectroscopy (EIS) and cyclic voltammograms (CV) demonstrated the fast electron transfer process between HRP and the electrode. The resulted H 2 O 2 biosensor exhibited a rapid response upon the addition of H 2 O 2 , a high sensitivity of 132 mA mM À1 cm À2 and a low detection limit of 6 10 À7 M. The linear range of the biosensor is from 5 10 À6 to 3.81 10 À3 M (r = 0.997). Such interesting G-Fe 3 O 4 composites with easy preparation and low cost can provide a novel matrix for enzyme immobilization to construct biosensors with higher performance.

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A Mediator‐Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode

Cited by 40 publications

References 37 publications

Protein functionalized carbon nanomaterials for biomedical applications

Protein functionalized carbon nanomaterials for biomedical applications

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite

Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites

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A Mediator‐Free Bienzyme Amperometric Biosensor Based on Horseradish Peroxidase and Glucose Oxidase Immobilized on Carbon Nanotube Modified Electrode

Cited by 40 publications

References 37 publications

Protein functionalized carbon nanomaterials for biomedical applications

Protein functionalized carbon nanomaterials for biomedical applications

Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite

Preparation and Application of Mediator‐Free H2O2 Biosensors of Graphene‐Fe3O4 Composites

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Preparation and Application of Mediator‐Free H₂O₂ Biosensors of Graphene‐Fe₃O₄ Composites