“…In addition, to gaining more information about the interaction prodrug/phospholipid, the experiments have also been carried out using squalene (2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene) and squalene acid (1,1 ,2-tris-nor-squalenic acid) used to allow the conjugation with gemcitabine (Scheme 1). Differential scanning calorimetry (DSC) is a nonperturbative technique largely employed to detect the effects exerted by biomolecules on the lipid bilayers of a cell-like membrane in the processes of entrapment and release inside lipid vesicles [9,10]. Lipid membranes (multilamellar vesicles, MLV, are usually employed as synthetic simplified biomembranes models) undergo a sharp phase transition from an ordered gel-like structure (L β ) to a disordered fluid-like structure (L α ).…”