A mathematical model to describe in vitro kinetics of H2 gas accumulation

Wang, M.; Janssen, Peter H.; Sun, Xuezhao; Muetzel, S.; Tavendale, Michael H.; Tan, Zhi Liang; Pacheco, D.

doi:10.1016/j.anifeedsci.2013.05.002

Cited by 41 publications

(46 citation statements)

References 27 publications

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“…FRD 0 and T 0.5 indexes reflect the rate of degradation during early incubation (< 12 h) and the required incubation time to reach half of the maximum gas production, respectively. Generally speaking, the faster FRD 0 is, the shorter T 0.5 becomes (Wang et al 2013). We found that FRD 0 for maize stover was significantly higher than for rice straw, i.e.…”

Section: In Vitro Gas Production Characteristics and Ch 4 Productionmentioning

confidence: 56%

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Effects ofLactobacillus acidophilussupplementation for improvingin vitrorumen fermentation characteristics of cereal straws

Chen

Ren

Zhou

et al. 2016

Italian Journal of Animal Science

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We explored and compared the effects of four doses (0, 0.25 Â 10 7 , 0.50 Â 10 7 , and 0.75 Â 10 7 cfu/mL) of Lactobacillus acidophilus supplementation on ruminal fermentation characteristics of maize stover and rice straw. The maximum gas production (Vf) for maize stover and the rate of gas production during early incubation (FRD 0 ) for rice straw were quadratic (p < .05) and linear (p < .01), respectively, and increased when supplemented with L. acidophilus. The L. acidophilus supplementation increased ruminal NH 3 -N concentration for maize stover (p < .01) and rice straw (p < .05), respectively. The supplementation level of L. acidophilus did not affect the production of volatile fatty acids (VFAs) except for valeric acid, nor did it affect CH 4 production, IVDMD, IVNDFD, ruminal pH value, total volatile fatty acids (TVFAs) or the ratio of acetic to propionic acid (A:P). As a fermentation substrate, when compared to rice straw, maize stover increased in vitro gas production parameters (e.g. V f , k, FRD 0 and t 0.5 ), IVDMD, IVNDFD, ruminal NH 3 -N concentration and TVFAs, whereas there was no (p > .05) difference in CH 4 production, ruminal pH value or A:P between the two substrates. The results indicate that L. acidophilus increase in vitro gas production and ruminal NH 3 -N concentration when using either fermentation substrate, with the greatest increase observed at a dosage of 0.75 Â 10 7 cfu/mL. Additionally, maize stover is superior to rice straw as a fermentation substrate or forage for ruminants. The present positive in vitro results should be tested using in vivo experiments in future.ARTICLE HISTORY

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Section: In Vitro Gas Production Characteristics and Ch 4 Productionmentioning

confidence: 56%

“…FRD 0 was used to calculate the initial fractional rate of degradation (/h) as follows (Wang et al 2013):…”

Section: Calculation and Statistical Analysismentioning

confidence: 99%

Effects ofLactobacillus acidophilussupplementation for improvingin vitrorumen fermentation characteristics of cereal straws

Chen

Ren

Zhou

et al. 2016

Italian Journal of Animal Science

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“…However, the methane eructated by ruminants contains only minute amounts of H 2 ; the concentration of dissolved H 2 in the rumen is near 1 μM (8), equivalent to a H 2 partial pressure of near 140 Pa. Because at 1 μM, H 2 formation from most substrates in the rumen is exergonic (9), the low H 2 concentration indicates that H 2 is consumed in the rumen by the methanogens more rapidly than it is formed by other microorganisms (10). The H 2 concentration increases substantially only when methane formation from H 2 and CO 2 is specifically inhibited by more than 50% (10,11). Already a small increase in the H 2 concentration (8) leads to both down-regulation of H 2 -generating pathways (12) and up-regulation of H 2 -neutral and H 2 -consuming pathways such as propionate formation, resulting in additional energy…”

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confidence: 99%

Mode of action uncovered for the specific reduction of methane emissions from ruminants by the small molecule 3-nitrooxypropanol

Duin

Wagner

Shima

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

208

188

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Ruminants, such as cows, sheep, and goats, predominantly ferment in their rumen plant material to acetate, propionate, butyrate, CO2, and methane. Whereas the short fatty acids are absorbed and metabolized by the animals, the greenhouse gas methane escapes via eructation and breathing of the animals into the atmosphere. Along with the methane, up to 12% of the gross energy content of the feedstock is lost. Therefore, our recent report has raised interest in 3-nitrooxypropanol (3-NOP), which when added to the feed of ruminants in milligram amounts persistently reduces enteric methane emissions from livestock without apparent negative side effects [Hristov AN, et al. (2015) Proc Natl Acad Sci USA 112(34):10663–10668]. We now show with the aid of in silico, in vitro, and in vivo experiments that 3-NOP specifically targets methyl-coenzyme M reductase (MCR). The nickel enzyme, which is only active when its Ni ion is in the +1 oxidation state, catalyzes the methane-forming step in the rumen fermentation. Molecular docking suggested that 3-NOP preferably binds into the active site of MCR in a pose that places its reducible nitrate group in electron transfer distance to Ni(I). With purified MCR, we found that 3-NOP indeed inactivates MCR at micromolar concentrations by oxidation of its active site Ni(I). Concomitantly, the nitrate ester is reduced to nitrite, which also inactivates MCR at micromolar concentrations by oxidation of Ni(I). Using pure cultures, 3-NOP is demonstrated to inhibit growth of methanogenic archaea at concentrations that do not affect the growth of nonmethanogenic bacteria in the rumen.

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“…The vented gas entered a gas chromatograph (Agilent 7890 A, Agilent Inc., Palo Alto, CA, USA) for measuring methane and hydrogen gas concentrations . Methane and gas hydrogen production was calculated according to the equation described by Wang et al . The in vitro fermentation was terminated after 72 h of incubation to collect liquid, solid and microbial samples.…”

Section: Methodsmentioning

confidence: 99%

Urea plus nitrate pretreatment of rice and wheat straws enhances degradation and reduces methane production inin vitroruminal culture

Zhang

Wang

et al. 2018

J Sci Food Agric

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A mathematical model to describe in vitro kinetics of H2 gas accumulation

Cited by 41 publications

References 27 publications

Effects ofLactobacillus acidophilussupplementation for improvingin vitrorumen fermentation characteristics of cereal straws

Effects ofLactobacillus acidophilussupplementation for improvingin vitrorumen fermentation characteristics of cereal straws

Mode of action uncovered for the specific reduction of methane emissions from ruminants by the small molecule 3-nitrooxypropanol

Urea plus nitrate pretreatment of rice and wheat straws enhances degradation and reduces methane production inin vitroruminal culture

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