Urea plus nitrate pretreatment of rice and wheat straws enhances degradation and reduces methane production in<i>in vitro</i>ruminal culture

Zhang, Xiumin; Wang, Min; Wang, Rong; Ma, Zhiyuan; Long, Donglei; Mao, Hongxiang; Wen, Jie; Bernard, L.; Beauchemin, K. A.; Tan, Zhiliang

doi:10.1002/jsfa.9056

Cited by 22 publications

(30 citation statements)

References 38 publications

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“…It has been reported that UN pretreatment improved ruminal degradation of rice straw measured in in vitro batch incubation [9]. The present in vivo study also indicates that goats fed UN had greater total-tract digestibility of NDF than those fed control.…”

Section: Discussionsupporting

confidence: 75%

“…A shortage of [H] would result in reduced methanogenesis. Our previous in vitro study found that urea plus nitrate pretreatment for rice straw decreased CH 4 production with a reduction of acetate to propionate ratio [9]. van Zijderveld et al [38] observed that nitrate supplementation caused CH 4 inhibition, which was accompanied by increased propionate molar proportion at the expense of acetate and butyrate.…”

Section: Discussionmentioning

confidence: 99%

“…Straw was pretreated with urea (34 g/kg straw dry matter [DM]) and 6 g/kg DM of ammonium nitrate (to supply 4.7 nitrate g/kg straw DM) for the urea plus nitrate treatment (UN). The pretreatment procedure was fully described by Zhang et al [9]. Briefly, urea and ammonium nitrate were dissolved in water (40%, w/v ) and sprayed onto the straw to achieve the appropriate concentrations.…”

Section: Methodsmentioning

confidence: 99%

“…Nitrate supplementation has been identified as a possible strategy to reduce CH 4 emissions [6, 7], because nitrate reduction to ammonia incorporates metabolic hydrogen ([H]) away from methanogenesis [8]. Urea plus nitrate pretreatment has been reported to increase degradation and reduce CH 4 production of rice straw in vitro [9], and further verification of these combined effects in vivo is needed.…”

Section: Introductionmentioning

confidence: 99%

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Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats

Zhang

Medrano

Wang

et al. 2019

J Animal Sci Biotechnol

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BackgroundUrea pretreatment is an efficient strategy to improve fiber digestibility of low quality roughages for ruminants. Nitrate and oil are usually used to inhibit enteric methane (CH4) emissions from ruminants. The objective of this study was to examine the combined effects of urea plus nitrate pretreated rice straw and corn oil supplementation to the diet on nutrient digestibility, nitrogen (N) balance, CH4 emissions, ruminal fermentation characteristics and microbiota in goats. Nine female goats were used in a triple 3 × 3 Latin Square design (27 d periods). The treatments were: control (untreated rice straw, no added corn oil), rice straw pretreated with urea and nitrate (34 and 4.7 g/kg of rice straw on a dry matter [DM] basis, respectively, UN), and UN diet supplemented with corn oil (15 g/kg soybean and 15 g/kg corn were replaced by 30 g/kg corn oil, DM basis, UNCO).ResultsCompared with control, UN increased neutral detergent fiber (NDF) digestibility (P < 0.001) and copies of protozoa (P < 0.001) and R. albus (P < 0.05) in the rumen, but decreased N retention (−21.2%, P < 0.001), dissolved hydrogen concentration (−22.8%, P < 0.001), molar proportion of butyrate (−18.2%, P < 0.05), (acetate + butyrate) to propionate ratio (P < 0.05) and enteric CH4 emissions (−10.2%, P < 0.05). In comparison with UN, UNCO increased N retention (+34.9%, P < 0.001) and decreased copies of protozoa (P < 0.001) and methanogens (P < 0.001). Compared with control, UNCO increased NDF digestibility (+8.3%, P < 0.001), reduced ruminal dissolved CH4 concentration (−24.4%, P < 0.001) and enteric CH4 emissions (−12.6%, P < 0.05).ConclusionsA combination of rice straw pretreated with urea plus nitrate and corn oil supplementation of the diet improved fiber digestibility and lowered enteric CH4 emissions without negative effects on N retention. These strategies improved the utilization of rice straw by goats.

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Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats

Zhang

Medrano

Wang

et al. 2019

J Animal Sci Biotechnol

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“…Generally, inclusion of high level of concentrate in the diet represents higher content of readily fermentable substance (e.g., starch) than that of high forage diets. Previous studies demonstrated that starch-rich diets reduced ruminal pH and H 2 concentration, and shifted fermentation patter towards to an increased propionate formation, which would depress the activity of methanogens and consequently reduce CH 4 emissions [30,31]. Moreover, the composition and structure of ruminal methanogens was demonstrated to differ across heifer physiological stages, which would affect the enteric CH 4 emissions of heifers [32].…”

Section: Discussionmentioning

confidence: 99%

Effects of Dietary Forage Proportion on Feed Intake, Growth Performance, Nutrient Digestibility, and Enteric Methane Emissions of Holstein Heifers at Various Growth Stages

Dong

Diao

2019

Animals

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Simple SummaryEnteric methane (CH4) emission from ruminants is a large source of anthropogenic greenhouse gas production, which is an inevitable by-product when feedstuff is digested and fermented in the rumen, representing approximately 7% of dietary energy loss. Although the Chinese government has committed to reduce CH4 emissions under the requirement of the Copenhagen Accord (2009), there is lack of accurate CH4 emission data from young cows as the guideline of IPCC gives little consideration to the variations of geographic conditions, animal physiology stages, and dietary components of dairy production system. Our study investigated the effects of different dietary forage-to-concentrate on feed intake, growth performance, nutrient digestibility, and enteric CH4 emissions of Holstein heifers under various growth stage, and developed the prediction equations using production and emission data. Our results demonstrated that enteric CH4 emission was significantly affected by dietary composition and physiological condition; results obtained from the current study will be of great importance for development of regional or national emission inventories and mitigation approaches for heifers at specific growth stage.AbstractEnteric methane (CH4) emissions from young ruminants contribute to a substantial proportion of atmospheric CH4 accumulation. Development of emission inventory and mitigation approaches needs accurate estimation of individual emission from animals under various physiological conditions and production systems. This research investigated the effect of different dietary concentrate contents on feed intake, growth performance, nutrient digestibility and CH4 emissions of heifers at various stages, and also developed linear or non-linear prediction equations using data measured by sulphur hexafluoride tracer technique. Increasing dietary concentrate contents increased feed intake and growth rate, enhanced nutrient digestibility, and reduced enteric CH4 emissions. Heifers at the age of 9, 12, and 15 months with an average weight of 267.7, 342.1, and 418.6 kg produced 105.2, 137.4, and 209.4 g/day of CH4, and have an average value of CH4 energy per gross energy intake (Ym) 0.054, 0.064, 0.0667, respectively. Equations relating CH4 emission values with animal and feed characteristics were developed with high determination coefficients for heifers at different growth stages. Dietary concentrate contents had significant influence on overall performance of heifers. These data can be used to develop regional or national emission inventories and mitigation approaches for heifers under various production regimes in China.

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Mixed ensiling plus nitrate destroy fiber structure of rape straw, increase degradation, and reduce methanogenesis through in vitro ruminal fermentation

Pu,

Zhang,

et al. 2024

J Sci Food Agric

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BACKGROUNDBetter utilization of rape straw can provide alternative strategies for sustainable ruminant and food production. The research reported here investigated changes in the carbohydrate composition of rape straw as a result of mixed ensiling with whole‐crop corn or inoculated with nitrate, and the consequent effects on ruminal fermentation through in vitro batch culture. The three treatments included: rape straw and corn silage (RSTC), and ensiling treatment of rape straw with whole‐crop corn (RSIC) or with calcium nitrate inoculation (RSICN).RESULTSEnsiling treatment of rape straw and whole‐crop corn or plus nitrate enriched lactic acid bacteria and lactate. The treatments broke the fiber surface connections of rape straw, leading to higher neutral detergent soluble (NDS) content and lower fiber content. Ensiling treatments led to greater (P < 0.05) dry matter degradation (DMD), molar proportions of propionate and butyrate, relative abundance of the phylum Bacteroidetes and genus Prevotella, and lower (P < 0.05) methane production in terms of g kg−1 DMD, molar proportions of acetate, and lower acetate to propionate ratio than the RSTC treatment. The RSICN treatment led to the lowest (P < 0.05) hydrogen concentration and methane production among the three treatments.CONCLUSIONEnsiling treatments of rape straw and whole‐crop corn destroy the micro‐structure of rape straw, promote substrate degradation by enriching the phylum Bacteroidetes and the genus Prevotella, and decrease methane production by favoring propionate and butyrate production. Nitrate inoculation in the ensiling treatment of rape straw and whole‐crop corn further decreases methane production without influencing substrate degradation by providing an additional hydrogen sink. © 2023 Society of Chemical Industry.

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Urea plus nitrate pretreatment of rice and wheat straws enhances degradation and reduces methane production inin vitroruminal culture

Cited by 22 publications

References 38 publications

Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats

Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats

Effects of Dietary Forage Proportion on Feed Intake, Growth Performance, Nutrient Digestibility, and Enteric Methane Emissions of Holstein Heifers at Various Growth Stages

Mixed ensiling plus nitrate destroy fiber structure of rape straw, increase degradation, and reduce methanogenesis through in vitro ruminal fermentation

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