A mathematical model of skeletal muscle regeneration with upper body vibration

Jones, Garrett; Smallwood, Cameron; Ruchti, Tysum; Blotter, Jonathan D.; Feland, J. Brent

doi:10.1016/j.mbs.2020.108424

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Interestingly, 30 Hz mechanical vibrations have been suggested to induce muscle hypertrophy in newborn mice in vivo and enhance terminal differentiation of satellite cells in vitro . Furthermore, high-frequency stimuli (30-90 Hz) have been suggested for musculoskeletal regenerative rehabilitation therapy ( Thompson et al, 2016 ; Jones et al, 2020 ) which may act through activation of a mechano-sensitive signalling pathway ( Uzer et al, 2015 ). Finally, a systematic review including trials that evaluated the effect of localised vibration on muscle strength in healthy individuals concluded that vibrations (8-300 Hz) can enhance the strength in healthy adults ( Alghadir et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation

Terrie

Burattini

Vlierberghe

et al. 2022

Front. Bioeng. Biotechnol.

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Skeletal muscle tissue engineering (SMTE) aims at the in vitro generation of 3D skeletal muscle engineered constructs which mimic the native muscle structure and function. Although native skeletal muscle is a highly dynamic tissue, most research approaches still focus on static cell culture methods, while research on stimulation protocols indicates a positive effect, especially on myogenesis. A more mature muscle construct may be needed especially for the potential applications for regenerative medicine purposes, disease or drug disposition models. Most efforts towards dynamic cell or tissue culture methods have been geared towards mechanical or electrical stimulation or a combination of those. In the context of dynamic methods, pulsed electromagnetic field (PEMF) stimulation has been extensively used in bone tissue engineering, but the impact of PEMF on skeletal muscle development is poorly explored. Here, we evaluated the effects of PEMF stimulation on human skeletal muscle cells both in 2D and 3D experiments. First, PEMF was applied on 2D cultures of human myoblasts during differentiation. In 2D, enhanced myogenesis was observed, as evidenced by an increased myotube diameter and fusion index. Second, 2D results were translated towards 3D bioartificial muscles (BAMs). BAMs were subjected to PEMF for varying exposure times, where a 2-h daily stimulation was found to be effective in enhancing 3D myotube formation. Third, applying this protocol for the entire 16-days culture period was compared to a stimulation starting at day 8, once the myotubes were formed. The latter was found to result in significantly higher myotube diameter, fusion index, and increased myosin heavy chain 1 expression. This work shows the potential of electromagnetic stimulation for enhancing myotube formation both in 2D and 3D, warranting its further consideration in dynamic culturing techniques.

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Section: Discussionmentioning

confidence: 99%

Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation

Terrie

Burattini

Vlierberghe

et al. 2022

Front. Bioeng. Biotechnol.

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Theoretical and numerical study of a skeletal muscle regeneration model with inflammatory response

Ruiz-Bolaños,

Kojouharov,

Solis

2024

International Journal of Computer Mathematics

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Effect of Localized Vibration Massage on Popliteal Blood Flow

Needs

Blotter

Cowan

et al. 2023

JCM

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There is a broad scope of literature investigating whole-body vibration (WBV) effects on blood flow (BF). However, it is unclear how therapeutic localized vibrations alter BF. Low-frequency massage guns are advertised to enhance muscle recovery, which may be through BF changes; however, studies using these devices are lacking. Thus, the purpose of this study was to determine if popliteal artery BF increases from localized vibration to the calf. Twenty-six healthy, recreationally active university students (fourteen males, twelve females, mean age 22.3 years) participated. Each subject received eight therapeutic conditions randomized on different days with ultrasound blood flow measurements. The eight conditions combined either control, 30 Hz, 38 Hz, or 47 Hz for a duration of 5 or 10 min. BF measurements of mean blood velocity, arterial diameter, volume flow, and heart rate were measured. Using a cell means mixed model, we found that both control conditions resulted in decreased BF and that both 38 Hz and 47 Hz resulted in significant increases in volume flow and mean blood velocity, which remained elevated longer than the BF induced by 30 Hz. This study demonstrates localized vibrations at 38 Hz and 47 Hz significantly increase BF without affecting the heart rate and may support muscle recovery.

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A mathematical model of skeletal muscle regeneration with upper body vibration

Cited by 3 publications

References 21 publications

Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation

Enhancing Myoblast Fusion and Myotube Diameter in Human 3D Skeletal Muscle Constructs by Electromagnetic Stimulation

Theoretical and numerical study of a skeletal muscle regeneration model with inflammatory response

Effect of Localized Vibration Massage on Popliteal Blood Flow

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