A Lysosome‐Targeting AIEgen for Autophagy Visualization

Abstract

In this work, a morpholine-functionalized aggregation-induced emission luminogen (AIEgen), AIE-LysoY, is reported for lysosomal imaging and autophagy visualization. To attain outstanding imaging contrast, AIE-LysoY is equipped with excited state intramolecular proton transfer (ESIPT) characteristic. AIE-LysoY provides a new platform for lysosome visualization with good biocompatibility, large Stokes shift, superior signal-to-noise ratio, and high photostability.

Two-photon luminescent metal complexes for bioimaging and cancer phototherapy

“…However, the impact of photostability is not considered by this TPA action cross-section [34]. Photostability is one of the most important criteria for developing fluorescent imaging agents [45]. Poor photostability will significantly limit the applications of a fluorescent probe.…”

Aggregation‐Induced Emission: Lighting up Cells, Revealing Life!

“…Inspired by the interesting work described above, Tang and co‐workers have also designed another lysosome‐targeting probe, AIE‐LysoY, which has AIE and ESIPT characteristics for tracing lysosomes and visualizing autophagy (Figure f) . The maximum absorption is at 390 nm and the strong emission is at 565 nm in water.…”

“…Lysosomes play a critical role in the autophagy process, so visualizing lysosomal activities is very important for research into autophagy‐related disease. As mentioned in Section , the lysosome‐targeted probe AIE‐LysoY has been utilized for monitoring the autophagy process . The ability of the probe to track the autophagy process in real time has also been examined.…”

Optical Imaging Paves the Way for Autophagy Research

“…and nanomaterials specifically located in autophagic structures. For example, aggregationinduced emission fluorogen and luminogen have been developed as bioprobes for tracking the mitochondria and lysosome, respectively, in the autophagy process with good specificity, biocompatibility, and photostability [45,46].…”