A Luminogen with Aggregation‐Induced Emission Characteristics for Wash‐Free Bacterial Imaging, High‐Throughput Antibiotics Screening and Bacterial Susceptibility Evaluation

Abstract

A luminogen with aggregation-induced emission characteristics is reported for bacterial imaging and antibiotics screening studies. The luminogen can light up bacteria in a wash-free manner, which simplifies the imaging process and increases its accuracy in bacterial detection. It can also be applied to high-throughput screening of antibiotics and fast evaluation of bacterial susceptibility, giving reliable results in less than 5 h.

Design and synthesis of theranostic antibiotic nanodrugs that display enhanced antibacterial activity and luminescence

“…2D). These observations are consistent with many other AIE-active luminogens having donor-acceptor structures (21,22,37,(39)(40)(41), where the aggregation hampers the intramolecular motion between the donor and the acceptor, hence leading to enhanced fluorescent emission as well as a blue shift in the emission peak (15). Spherical nanoaggregates were observed at high water content (≥99 vol %) (Fig.…”

“…The significance of this work includes: (i) a modular synthetic strategy for the preparation of ciprofloxacin derivatives in high yields in one step at room temperature without any catalysts; (ii) stable nanoaggregates, prepared following the principle of aggregation-induced emission, and successfully used as fluorescent organic dots to image bacteria; and (iii) formulation of nanodrugs that showed aggregation-enhanced antibacterial activities. intramolecular motions in AIE often involves supramolecular interactions, which result in fairly stable aggregates (19)(20)(21)(22). Indeed, aqueous suspensions of AIE aggregates can be stable over several months in the absence of surfactants (23).…”

Circularly Polarized Luminescence and a Reflective Photoluminescent Chiral Nematic Liquid Crystal Display Based on an Aggregation‐Induced Emission Luminogen

“…Recently, a new class of non-planar organic luminogens with aggregate-induced emission (AIE) properties has attracted tremendous attention. [42][43][44][45][46][47] As a fantastic photophysical phenomenon, AIE is diametrically opposed to the ACQ effect. Usually containing a structure with molecular rotors, AIE molecules are weakly fl uorescent or non-fl uorescent in the solution state due to active intramolecular rotation that consumes energy from the excited state, but become brightly fl uorescent induced by aggregation or high viscosity.…”

Recent Advances and Future Prospects on Adaptive Biomaterials for Antimicrobial Applications

“…For example, when exposed to bacteria, the surface plasmon spectrum of AuNP, such as surface plasmon resonance (SPR), two‐photon scattering (TPS), surface‐enhanced Raman scattering (SERS), could be greatly changed and have been used as optical signal transduction to detect pathogens . During the past decades, several other novel nanoparticle‐based bacteria detection systems, including magnetic NPs (MNPs), quantum dots (QDs), graphene oxides (GOs), upconversion NPs (UCNPs), and aggregation‐induced emission (AIE), have been developed. Though they are fundamentally simple, these aggregation methods are commonly powerful in detecting the food‐ or water‐borne pathogens and are quite challenging to be developed into bacterial infection disease diagnosis, especially in patients.…”