We report the modular formulation of ciprofloxacin-based pure theranostic nanodrugs that display enhanced antibacterial activities, as well as aggregation-induced emission (AIE) enhancement that was successfully used to image bacteria. The drug derivatives, consisting of ciprofloxacin, a perfluoroaryl ring, and a phenyl ring linked by an amidine bond, were efficiently synthesized by a straightforward protocol from a perfluoroaryl azide, ciprofloxacin, and an aldehyde in acetone at room temperature. These compounds are propeller-shaped, and upon precipitation into water, readily assembled into stable nanoaggregates that transformed ciprofloxacin derivatives into AIE-active luminogens. The nanoaggregates displayed increased luminescence and were successfully used to image bacteria. In addition, these nanodrugs showed enhanced antibacterial activities, lowering the minimum inhibitory concentration (MIC) by more than one order of magnitude against both sensitive and resistant Escherichia coli. The study represents a strategy in the design and development of pure theranostic nanodrugs for combating drug-resistant bacterial infections.nanodrugs | aggregation-induced emission | fluoroquinolones
The ability of proteins to form hierarchical structures through self-assembly provides an opportunity to synthesize and organize nanoparticles. Ordered nanoparticle assemblies are a subject of widespread interest due to the potential to harness their emergent functions. In this work, the toroidal-shaped form of the protein peroxiredoxin, which has a pore size of 7 nm, was used to organize iron oxyhydroxide nanoparticles. Iron in the form of Fe was sequestered into the central cavity of the toroid ring using metal-binding sites engineered there and then hydrolyzed to form iron oxyhydroxide particles bound into the protein pore. By precise manipulation of the pH, the mineralized toroids were organized into stacks confining one-dimensional nanoparticle assemblies. We report the formation and the procedures leading to the formation of such nanostructures and their characterization by chromatography and microscopy. Electrostatic force microscopy clearly revealed the formation of iron-containing nanorods as a result of the self-assembly of the iron-loaded protein. This research bodes well for the use of peroxiredoxin as a template with which to form nanowires and structures for electronic and magnetic applications.
Spin‐wave based technologies that use collective oscillation of electrons termed magnons have been proposed for future computing landscapes due to their low energy consumption and high data transfer speeds. Magnonic crystals, materials with magnetic properties periodically varied in space, are central to such technologies. However, they are currently limited by the lithography techniques used for the magnetic patterning. To address this issue, bottom‐up self‐assembly using polymer templates to order magnetic cargo is presented. In this work, block copolymer micelles are used as templates to direct the organization of polyoxometalate (POM) molecules into organized assemblies. The structural organization of these assemblies is evaluated using microscopy and scattering techniques. The organized POM assemblies are demonstrated to modulate spin‐waves excited in permalloy thin films. This work demonstrates the first use of a bottom‐up approach to realize the fabrication of a magnonic assembly at the nanoscale. It further paves the way to achieve magnon‐mediated self‐assembled computing architectures.
The fluorescence properties of AIE‐active N‐amidinated fluoroquinolones, efficiently obtained by a perfluoroaryl azide–aldehyde–amine reaction, have been studied. The fluorophores were discovered to elicit a highly sensitive fluorescence quenching response towards guest molecules with hydrogen‐bond‐donating ability. This effect was evaluated in a range of protic/aprotic solvents with different H‐bonding capabilities, and also in aqueous media. The influence of acid/base was furthermore addressed. The hydrogen‐bonding interactions were studied by IR, NMR, UV/Vis and time‐resolved fluorescence decay, revealing their roles in quenching of the fluorescence emission. Due to the pronounced quenching property of water, the N‐amidinated fluoroquinolones could be utilized as fluorescent probes for quantifying trace amount of water in organic solvents.
Hydrogels are excellent soft materials to interface with biological systems. Precise control and tunability of dissipative properties of gels are particularly interesting in tissue engineering applications. In this work, we...
Since its discovery in 2006, the DNA origami technique has revolutionized bottom-up nanofabrication. This technique is simple yet versatile and enables the fabrication of nanostructures of almost arbitrary shapes. Furthermore, due to their intrinsic addressability, DNA origami structures can serve as templates for the arrangement of various nanoscale components (small molecules, proteins, nanoparticles, etc.) with controlled stoichiometry and nanometerscale precision, which is often beyond the reach of other nanofabrication techniques. Despite the multiple benefits of the DNA origami technique, its applicability is often restricted by the limited stability in application-specific conditions. This Review provides an overview of the strategies that have been developed to improve the stability of DNA-origami-based assemblies for potential biomedical, nanofabrication, and other applications.
Orientational control of anisotropic plasmonic nanoparticles is an attractive proposition to generate dynamic plasmonic responses. Particularly, the use of light as a stimulus to modulate the orientation is extremely useful owing to its spatiotemporal operative ability. This work showcases a light-mediated approach to tune the orientational features of gold nanorods in DNAengineered hydrogel materials. The strategy relies on the use of visible-lightinduced photothermal effects to cause deformation of the hydrogel matrix, resulting in temperature-controlled polarization-dependent optical responses whose anisotropy features are highly adaptive to the nature of DNA crosslinks. The visible-light-mediated approach showcased here can open novel avenues to create dynamic light-responsive materials with reconfigurable plasmonic responses.
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