A low level of lysophosphatidic acid in human gingival crevicular fluid from patients with periodontitis due to high soluble lysophospholipase activity: Its potential protective role on alveolar bone loss by periodontitis

Hashimura, Satoru; Kido, Jun‐ichi; Matsuda, Ryozo; Yokota, Mika; Matsui, Hirokazu; Inoue-Fujiwara, Manami; Inagaki, Yuji; Hidaka, Mayumi; Tanaka, Tamotsu; Tsutsumi, Toshihiko; Nagata, Toshihiko; Tokumura, Akira

doi:10.1016/j.bbalip.2020.158698

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…For LPA, conflicting results were obtained. In particular, one study 37 reported a marked increase in CP and AgP groups compared with H controls for all the four tested LPA species; whereas another study 30 obtained significantly higher LPA concentrations in H subjects, although numerical values were not given.…”

Section: Findings From Specific Metabolitesmentioning

confidence: 93%

“…31,33,34,38,40 The majority of studies used paper strips (Periopaper) to collect GCF; while few studies used endodontic paper points 37 and microcapillary pipettes. 39 Eleven studies reported a sampling duration of 30 s; 26,27,29,[31][32][33][34][35][36]38,40 2 studies of 10 seconds; 28,30 while 2 other studies did not mention it. 37,39 Nine studies used Periotron 8000 for volume measurement.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…37,39 Nine studies used Periotron 8000 for volume measurement. 26,[29][30][31][32]35,36,38,40 Mass spectrometry (MS), either coupled with liquid or gas chromatography (LC or GC), was the preferred method for untargeted analysis; while for targeted analysis the detection methods were strictly dependent on the metabolite tested as summarized in the last column of Table 1.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Malondialdehyde (MDA) was the most investigated molecule, recurring in four studies 31,32,38,40 followed by 8-hydroxy-deoxyguanosine (8-OHdG) in three studies, 33,35,36 and lysophosphatidic acid (LPA) in two studies. 30,37 LPA precursors, lysophosphatidylcholine (LPC), and lysophosphatidylethanolamine (LPE), together with choline, 4-hydroxynonenal (4-HNE), neopterin, and reduced and oxidized glutathione were only evaluated in one article. Analysis of diagnostic accuracy was performed in only one study.…”

Section: Study Characteristicsmentioning

confidence: 99%

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Metabolomics of gingival crevicular fluid to identify biomarkers for periodontitis: A systematic review with meta‐analysis

Baima

Corana

Iaderosa

et al. 2021

J of Periodontal Research

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The present systematic review aimed to examine periodontitis-specific biomarkers in the gingival crevicular fluid (GCF) that could have a diagnostic relevance, and to provide a qualitative assessment of the current literature. Metabolites are reliable indicators of pathophysiological statuses, and their quantification in the GCF can provide an outlook of the changes associated with periodontitis and have diagnostic value. Relevant studies identified from PubMed, Embase, Cochrane Library, and Scopus databases were examined to answer the following PECO question: "In systemically healthy individuals, can concentration of specific metabolites in the GCF be used to discriminate subjects with healthy periodontium (H) or gingivitis from patients with periodontitis (P) and which is the diagnostic accuracy?" Quality of included studies was rated using a modified version of the QUADOMICS tool. Meta-analysis was conducted whenever possible. After the screening of 1,554 titles, 15 studies were selected, with sample size ranging from 30 to 93 subjects. Eleven studies performed targeted metabolomics analysis and provided data for 10 metabolites. Among the most consistent markers, malondialdehyde levels were found higher in the P group compared with H group (SMD = 2.86; 95% CI: 1.64, 4.08). Also, a significant increase of 8-hydroxy-deoxyguanosine, 4-hydroxynonenal, and neopterin was detected in periodontally diseased sites, while glutathione showed an inverse trend. When considering data from untargeted metabolomic analysis in four studies, more than 40 metabolites were found significantly discriminant, mainly related to amino acids and lipids degradation pathways. Notably, only one study reported measures of diagnostic accuracy. Several metabolites were differentially expressed in GCF of subjects across different periodontal conditions, having a major potential for investigating periodontal pathophysiology and for site-specific diagnosis. Oxidative stress-related molecules, such as malondialdehyde and 8-hydroxy-deoxyguanosine, were the most consistently associated to periodontitis (PROSPERO CRD42020188482).

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Section: Findings From Specific Metabolitesmentioning

confidence: 93%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

See 2 more Smart Citations

Metabolomics of gingival crevicular fluid to identify biomarkers for periodontitis: A systematic review with meta‐analysis

Baima

Corana

Iaderosa

et al. 2021

J of Periodontal Research

View full text Add to dashboard Cite

show abstract

“…Several other components of GCF are also shown to reflect the periodontal disease status that includes neutrophil elastase [42], periostin [43], Chitinase-3-like protein 1 (also known as YKL-40) [44], lysophosphatidic acid (LPA) [45], human beta-defensins (hBDs) [46], hypoxia-inducible factor-1α (HIF-1α) [47], osteoprotegerin [48], and anti-Hsp-70 (heat shock protein family A) [49]. In light of innumerable proactive studies, it has already been suggested that this is an ideal fluid as a strong and unmatchable latest chemical gizmo that cross matches the clinical parameters too.…”

Section: Gcf As a Biomarkermentioning

confidence: 99%

Gingival Crevicular Fluid (GCF): A Diagnostic Tool for the Detection of Periodontal Health and Diseases

et al. 2021

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The methodologies applicable for the evaluation of periodontal associated diseases are constantly evolving to provide quick, realistic, and scientifically proven results. Trends in the past followed a clinical evaluation of periodontal tissues and radiographic-based reports that formed the foundation for detection of diseases involving the structures supporting the teeth. As the confines and limitations of conventional strategies became obvious over the passage of time, hand in hand variety of techniques have evolved and experimentally justified. These improvisations are based on an improved understanding of the periodontal-pathogenic cascade. Periodontal pathogenesis and a paradigm shift from disease understanding to disease prevention and treatment entail few prerequisites that demand the objectivity of diagnostics procedure that includes sensitivity and specificity along with an explanation of the intensity of the disease, Gingival crevicular fluid an oral bio-fluid resides in the close proximity with gingival tissues have been widely used to understand and differentiate the periodontal health and diseased status. The biomarkers present in the GCF can be a reliable tool to detect the minute changes seen in the disease processes. The GCF consists of various host and bacterial-derived products as well as biomarkers which in turn can be evaluated for the diagnosis, prognosis as well as management of the periodontal disease. Thus, the review aims at describing GCF as a potential oral biofluid helpful in differentiating periodontal health and disease status.

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Periodontitis: Clinical Aspects, Pathophysiology, Experimental Approaches and Emerging Therapies

Kang

Berthiaume

2021

Nano LIFE

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This review paper presents an overview of periodontal disease, beginning with clinical aspects including prevalence, etiology, disease progression mechanisms and implications for general health, followed by current diagnostic and therapeutic approaches. We then describe experimental models used for in vitro and in vivo investigations of periodontal disease, and emerging therapeutic approaches based on biomedical and tissue engineering concepts, nanotechnology, gene and stem cell therapies. We aim to provide bioengineers, tissue engineers and nanotechnology researchers an overall understanding of the state-of-the-art of periodontal disease to foster technological innovation based on interdisciplinary research and collaboration between physical and medical scientists.

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A low level of lysophosphatidic acid in human gingival crevicular fluid from patients with periodontitis due to high soluble lysophospholipase activity: Its potential protective role on alveolar bone loss by periodontitis

Cited by 6 publications

References 40 publications

Metabolomics of gingival crevicular fluid to identify biomarkers for periodontitis: A systematic review with meta‐analysis

Metabolomics of gingival crevicular fluid to identify biomarkers for periodontitis: A systematic review with meta‐analysis

Gingival Crevicular Fluid (GCF): A Diagnostic Tool for the Detection of Periodontal Health and Diseases

Periodontitis: Clinical Aspects, Pathophysiology, Experimental Approaches and Emerging Therapies

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