A Low‐Cost Laser‐Based Nano‐3D Polymer Printer for Rapid Surface Patterning and Chemical Synthesis of Peptide and Glycan Microarrays

Abstract

A low‐cost laser‐based printing setup is presented, which allows for the spot‐wise patterning of surfaces with defined polymer nanolayers. These nanolayer spots serve as a “solid solvent,” embedding different chemicals, chemical building blocks, materials, or precursors and can be stacked on top of each other. By melting the spot pattern, the polymer‐embedded molecules are released for chemical reaction. This enables researchers to quickly pattern a surface with different molecules and materials, mixing them directly on the surface for high‐throughput chemical synthesis to generate and screen diverse microarray libraries. In contrast to expensive ink‐jet or contact printing, this approach does not require premixing of inks, which enables in situ combinatorial mixing. Easy access and versatility of this patterning approach are shown by generating microarrays of various biomolecules, such as glycans for the first time, to screen interactions of antibodies and lectins. In addition, a layer‐by‐layer solid‐phase synthesis of peptides directly on the microarray is presented. Amino acid–containing nanolayers are repeatedly laser‐transferred and reacted with the functionalized acceptor surface in defined patterns. This simple system enables a reproducible array production, down to spot‐to‐spot distances of 100 µm, and offers a flexible and cheap alternative to expensive spotting robot technology.

Epitopes of Naturally Acquired and Vaccine‐Induced Anti‐Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution

“…Advances in technology development, which for example rely on the patterning of co‐polymers for the synthesis of biomolecules, [ 24 ] make the rapid production of peptide microarrays feasible. [ 25–27 ] High‐density peptide microarrays have been used for the development of diagnostic biomarkers, for example, in Malaria, Zika virus, and Chagas disease, [ 28–30 ] as well as in the analysis of therapeutic antibodies against C. difficile . [ 31 ] Additionally, epitopes recognized by antibodies elicited after vaccination against infectious diseases, such as Tuberculosis, Malaria, and Tetanus, can be identified.…”

Glycan Nanobiosensors

“…The technology also makes possible the mixing of different molecules on the surface ( Figure 5 C). The proof of concept was also tested by the binding of Con A to a mannose-patterned interface [ 55 ].…”

Trendbericht: Makromolekulare Chemie

“…Ähnlich wie bei einer Schreibmaschine überträgt ein Laser kleinste Polymernanoschichten, die bestimmte Farben in Form chemischer Bausteine enthalten, in fein definierte Muster. 56) BB…”