A Low‐Cost, High‐Throughput Polyacrylamide Gel Electrophoresis System for Genotyping with Microsatellite DNA Markers

Wang, D.; Shi, Jie; Carlson, S. R.; Cregan, Perry B.; Ward, Roxanne; Diers, Brian W.

doi:10.2135/cropsci2003.1828

Cited by 182 publications

(134 citation statements)

References 7 publications

(8 reference statements)

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“…Then, the machine was run according to the following setup: Initial denaturation at 95°C for 3 min; Denaturation at 94°C for 1 min; Annealing at 50-60°C for 1 min; Extension at 72°C for 1 min; completion of cycling program (40 cycles); Final extension at 72°C for 7 min and reaction were held at 4°C. The amplified products were separated on 6% polyacrylamide gels containing ethidium bromide (Wang et al, 2003). Molecular weight marker of 20 bp was run with the PCR products.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Patel¹,

Yadav²,

Avtar³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Section: Pcr Amplificationmentioning

confidence: 99%

Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Patel¹,

Yadav²,

Avtar³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…PCR products were detected on 6 % non-denaturing polyacrylamide gels using a DASG-400-50 electrophoresis system (C.B.S. Scientific Co., Del Mar, CA, USA) as described by Wang et al (2003). Gels stained with ethidium bromide were photographed, and scored under UV light.…”

Section: Dna Extraction and Marker Analysismentioning

confidence: 99%

Mapping soybean aphid resistance genes in PI 567598B

et al. 2013

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The soybean aphid (Aphis glycines Matsumura) has been a major pest of soybean [Glycine max (L.) Merr.] in North America since it was first reported in 2000. Our previous study revealed that the strong aphid resistance of plant introduction (PI) 567598B was controlled by two recessive genes. The objective of this study was to locate these two genes on the soybean genetic linkage map using molecular markers. A mapping population of 282 F 4:5 lines derived from IA2070 9 E06902 was evaluated for aphid resistance in a field trial in 2009 and a greenhouse trial in 2010. Two quantitative trait loci (QTLs) were identified using the composite and multiple interval mapping methods, and were mapped on chromosomes 7 (linkage group M) and 16 (linkage group J), respectively. E06902, a parent derived from PI 567598B, conferred resistance at both loci. In the 2010 greenhouse trial, each of the two QTLs explained over 30 % of the phenotypic variation. Significant epistatic interaction was also found between these two QTLs. However, in the 2009 field trial, only the QTL on chromosome 16 was found and it explained 56.1 % of the phenotypic variation. These two QTLs and their interaction were confirmed with another population consisting of 94 F 2:5 lines in the 2008 and 2009 greenhouse trials. For both trials in the alternative population, these two loci explained about 50 and 80.4 % of the total phenotypic variation, respectively. Our study shows that soybean aphid isolate used in the 2009 field trial defeated the QTL found on chromosome 7. Presence of the QTL on chromosome 16 conferred soybean aphid resistance in all trials. The markers linked to the aphid-resistant QTLs in PI 567598B or its derived lines can be used in marker-assisted breeding for aphid resistance.

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“…(1 ethidium bromide for 2 to 2.5 h (Wang et al 2003) and recorded on a digital gel documentation system.…”

Section: Ssr Analysismentioning

confidence: 99%

Genetic variability of Canadian elite cultivars of summer turnip rape (Brassica rapa L.) revealed by simple sequence repeat markers

Fu¹,

Gugel²

2009

Can. J. Plant Sci.

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. 2009. Genetic variability of Canadian elite cultivars of summer turnip rape (Brassica rapa L.) revealed by simple sequence repeat markers. Can. J. Plant Sci. 89: 865Á874. Canadian public oilseed breeding programs have played a leading role in the development of canola-quality Brassica rapa oilseed cultivars, but the genetic variability of the cultivars developed over the past 60 yr is poorly understood. Simple sequence repeat (SSR) markers were applied to assess the genetic variability of 294 plants representing one landrace introduced from Poland ca. 1940 and nine Canadian elite B. rapa cultivars released by Agriculture and Agri-Food Canada since 1964. Application of 18 SSR primer pairs detected 27 likely loci on five or more linkage groups and 123 polymorphic alleles. The allelic frequencies ranged from 0.003 to 0.997 and averaged 0.262. The estimates of mean heterozygosity for these cultivars ranged from 0.126 to 0.197 and averaged 0.165. Significant decreases of SSR alleles and average dissimilarities were observed over the 60 yr of breeding effort. The proportion of total SSR variation residing among the cultivars was 10.4%; between high vs. low erucic acid cultivars 5% and between high-vs. low-glucosinate cultivars 5%. Pairwise genetic differentiation ranged from 0.025 to 0.184 and averaged 0.104. Genetic clustering of these cultivars revealed AC Sunbeam to be a genetically unique cultivar, and two distinct groups of the other nine cultivars were separated by high-vs. low-glucosinate content. Several distinct genotypes, largely derived from the landrace Polish, were identified. These findings are useful for broadening the genetic base of elite B. rapa germplasm, selecting genetically diverse genotypes for synthetic and hybrid combinations, and conserving this germplasm. L'he´te´rozygotie moyenne estime´e pour ces cultivars varie de 0,126 a`0,197, pour une moyenne de 0,165. Les auteurs ont note´une diminution importante des alle`les SSR et des dissemblances moyennes durant les soixante anne´es qu'a dure´le programme d'hybridation. La variation des SSR s'e´tablissait a`10,4% pour l'ensemble des cultivars, soit 5% pour ceux ah aute ou a`faible concentration d'acide e´rucique et 5% pour ceux a`forte ou a`basse concentration de glucosinolates. La diffe´renciation ge´ne´tique par paire varie de 0,025 a`0,184, pour une moyenne de 0,104. Le regroupement ge´ne´tique des cultivars indique qu'AC Sunbeam est un cultivar unique, ge´ne´tiquement parlant; les neuf autres cultivars ont e´te´divise´s en deux groupes distincts selon leur concentration, faible ou e´leve´e, de glucosinolates. Plusieurs ge´notypes diffe´rents, de´coulant dans une large mesure de la race polonaise, ont pu eˆtre identifie´s. Ces re´sultats contribueront a`e´largir la base ge´ne´tique du mate´riel e´lite de B. rapa, la se´lection de ge´notypes diffe´rents en vue de la cre´ation de cultivars par synthe`se ou hybridation, ainsi que la conservation du mate´riel ge´ne´tique.

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A Low‐Cost, High‐Throughput Polyacrylamide Gel Electrophoresis System for Genotyping with Microsatellite DNA Markers

Cited by 182 publications

References 7 publications

Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Mapping soybean aphid resistance genes in PI 567598B

Genetic variability of Canadian elite cultivars of summer turnip rape (Brassica rapa L.) revealed by simple sequence repeat markers

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