Genetic Diversity Analysis for Salinity Tolerance in  Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Patel, Rekha; Yadav, Renu; Avtar, Ram; Jangra, Sumit; Boken, Geeta; Singh, Beant; Yadav, Neelam

doi:10.20546/ijcmas.2018.701.215

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…In our study, PIC value of the markers varied from minimum 0.2957 with primer SORF-73 (B-27) to a maximum 0.8768 with SR_9222 with an average of 0.6555. The average PIC values were comparable as reported (Patel et al, 2018) where the PIC value ranged from 0.427-0.730 with an average of 0.555 and Shyam et al, (2020) who reported polymorphism information content (PIC) value of the markers varied from minimum 0.51for SSR Na10-D07 to maximum 0.73 with primer BRMS-098 with a mean value of 0.62 (Table 5). PIC values reported by Bharti et al, (2018) ranged from 0.34 to 0.49 with an average of 0.41 while Gupta et al, (2014) reported low PIC value 0.281 for SSR markers during their study on molecular diversity analysis of mustard.…”

Section: Ssupporting

confidence: 84%

Analysis of Genetic Diversity among Brassica juncea Genotypes using Morpho-physiological and SSR Markers

Verma¹,

Tripathi²,

Tiwari³

et al. 2021

Int.J.Curr.Microbiol.App.Sci

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Section: Ssupporting

confidence: 84%

Analysis of Genetic Diversity among Brassica juncea Genotypes using Morpho-physiological and SSR Markers

Verma¹,

Tripathi²,

Tiwari³

et al. 2021

Int.J.Curr.Microbiol.App.Sci

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“…However, BG119 (0.206), BG116 (0.234) and BG135 (0.278) had low PIC values and could be considered as the least powerful markers. The average PIC value (0.519) observed in our study was consistent with previous estimates of SSR marker analysis in Indian mustard by Avtar et al (2016) [2] and Patel et al (2018) who reported average PIC values of 0.529 and 0.555, respectively. On the other hand, the observed value was higher than the values of 0.46, 0.28 and 0.31 reported by Turi et al (2012) [20] , Gupta et al (2014) [5] , and Sudan et al (2016) [17] , respectively.…”

Section: Results and Discussion Ssr Marker-based Divergence And Allelic Diversity Analysissupporting

confidence: 92%

Molecular diversity analysis using Simple sequence repeats (SSRs) in ‘A’ and ‘R’ lines of Ogura CMS system in Indian mustard

Kumar¹,

Avtar²,

Ahlawat³

et al. 2020

Int. J. Chem. Stud.

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Fifty-eight SSR primers were used for molecular diversity analysis among 45 'A' and 'R' lines (5 A lines and 40 restorers) of Indian mustard. Out of 58 primers 33 primers were found to be polymorphic. A total of 95 alleles were detected across all 45 genotypes. The PCR amplified products ranged from 70 bp to 350 bp in size. The number of alleles per locus varied from 2 to 5 with an average of 2.89. The PIC value varied from 0.206 to 0.749 with an average of 0.519. The highest PIC value was obtained for BG 132 (0.749) followed by BG 114 (0.744). The UPGMA cluster analysis suggested demarcation of 45 genotypes into six distinct groups in dendrogram wherein three groups had single genotype (restorer line) each. Cluster VI consisted of all five Ogura CMS lines only. In the remaining clusters, restorer lines were distributed. Based on similarity coefficient, cross combinations viz., RH 0749-OA & IOR-17, RH 0749-OA & IOR-20 and RH 119-OA & OR-17 were identified as diverse and might be suitable for using parent in heterosis exploitation in Indian mustard.

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“…Polymorphism information content (PIC) value of the markers varied from minimum 0.51 for SSR Na10-D07 to maximum 0.73 with primer BRMS-098 with a mean value of 0.62. In our study, the average PIC values were found to be higher than that of reported by Patel et al [29], where the PIC value ranged from 0.427-0.730 with an average of 0.555. PIC values reported by Bharti et al (2018) ranged from 0.34 to 0.49 with an average of 0.41.…”

Section: Discussioncontrasting

confidence: 86%

Characterization of Indian Mustard Germplasm on the Basis of Morphological Traits and SSR Markers

Rajpoot¹,

Tripathi²,

Tiwari³

et al. 2020

CJAST

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The genus Brassica is one of the most important oil seed crops in India with high degree of genetic diversity. In present study, genetic diversity was studied in forty germplasm lines and eight cultivars of Indian mustard using morphological traits and SSR markers. Morphological characters were taken for days to 50% flowering, days to maturity, plant height (cm), length of main raceme (cm), number of primary branches/plant, number of secondary branches/plant, number of silique per plant, number of seeds per silique, 1000 seed weight (g) and seed yield per plant (g). Total 50 SSR markers were used for characterization of these lines, out of which 7 SSR markers were highly polymorphic between all the germplasms of mustard. An UPGMA phonogram was constructed for all 48 Germplasms and the similarity coefficient ranged from 0.00 to 0.91. Number of alleles ranged from 3 to 4, genetic diversity ranged from 71% to 65% with average value of 67%, heterozygosity raged from 20 to 10% with average of 12% and PIC value for markers ranged from 0.65 to 0.59 with mean PIC value 0.61. All seven SSR primers showed PIC value above 0.5 (50%) indicating high genetic diversity in the studied plant material.

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Genetic Diversity Analysis for Salinity Tolerance in Indian Mustard [Brassica juncea (L.)] Using SSR Markers

Cited by 8 publications

References 33 publications

Analysis of Genetic Diversity among Brassica juncea Genotypes using Morpho-physiological and SSR Markers

Analysis of Genetic Diversity among Brassica juncea Genotypes using Morpho-physiological and SSR Markers

Molecular diversity analysis using Simple sequence repeats (SSRs) in ‘A’ and ‘R’ lines of Ogura CMS system in Indian mustard

Characterization of Indian Mustard Germplasm on the Basis of Morphological Traits and SSR Markers

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