A longitudinal study of <i>Escherichia coli</i> O157 in fourteen
 

cattle herds

Hancock, Dale D.; Besser, Thomas E.; Rice, Daniel H.; Herriott, Donald E.; Tarr, Phillip I.

doi:10.1017/s0950268896007212

Cited by 285 publications

(247 citation statements)

References 13 publications

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“…The prevalence of EHEC O157 in cattle has been reported to be 0.2-60.4% in the United States [10,13,23,28], 1.9% in Australia [7], and 4.2-66.7% in Europe [5,6,16,24]. In Japan, it was reported that the isolation rate of the EHEC was between 0.3% [12] and 6.5% [15] in faeces samples from animals delivered to a slaughterhouse.…”

Section: Shiga Toxin (Stx)-producing Escherichia Coli (Stec) Ismentioning

confidence: 99%

“…EHEC O157 was also isolated from environmental samples, though the isolation rate was low in comparison with that from faeces. Porter et al [21] and Cobbold et al [10] reported that EHEC O157 was more usually isolated from samples from slurry or in close proximity to the milking parlour. In this study, it was notable that EHEC O157 was detected not only in bedding and the feed trough being in direct contact with the cattle, but from the barn alley as well.…”

Section: Isolation and Prevalence Of Ehec O157 From Faeces Of Heifersmentioning

confidence: 99%

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A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

Ezawa

Gocho

Saitoh

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. A long-term study was performed on the prevalence of enterohemorrhagic Escherichia coli (EHEC) O157 in bovine faeces. The present study was conducted on heifers raised on a farm showing a high isolation rate of EHEC O157 in previous years. The p revalence of EHEC O157 isolated from faecal samples was 10.6% (222/2104), 5.6% (181/3225), and 5.6% (153/2744) from 1998 to 2000, respectively. The numbers of EHEC O157-positive heifers for the same 3 years were 46.3% (185/400), 36.8% (147/399), and 31.7% (130/410), respectively. The seasonal prevalence of EHEC O157 varied according to the year. Most positive heifers excreted the EHEC O157 only once during the survey, though it was excreted 2 or 3 times by some heifers. The results obtained in the present study showed that the farm examined was heavily contaminated with EHEC O157. It is assumed that EHEC O157 does not remain in individual catt le long-term, but does exist long-term on farms due to repeated infection.

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Section: Shiga Toxin (Stx)-producing Escherichia Coli (Stec) Ismentioning

confidence: 99%

Section: Isolation and Prevalence Of Ehec O157 From Faeces Of Heifersmentioning

confidence: 99%

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

Ezawa

Gocho

Saitoh

et al. 2004

The Journal of Veterinary Medical Science

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“…EHEC O157 can be isolated from various animals such as cattle [2,4,5,8,9,12,16,23], sheep [6], deer [1], and so on. In particular, it is often isolated from faeces of cattle showing no clinical signs of disease.…”

mentioning

confidence: 99%

“…In particular, it is often isolated from faeces of cattle showing no clinical signs of disease. It was reported that the prevalence of EHEC O157 in cattle was 0.2-28% in the United States [7,8,23], 1.9% in Australia [5], and 4.2-20% in Europe [2,4,9,16]. Therefore, cattle can harbour the organisms in their faeces and are regarded as a natural reservoir and source of infection [3].…”

mentioning

confidence: 99%

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Ezawa

Gocho

Kawata

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of enterohemorrhagic Escherichia coli (EHEC) O157 was examined in bovine faeces. EHEC O157 was isolated from the faeces of 42 (13.0%) of 324 cattle. Of the 4 farms and the facilities tested, the 3 farms and the facilities wer e found positive for EHEC O157. The highest isolation rate among the farms was 33.7%. The prevalence of EHEC O157 in heifers was higher than that in calves and other cattle. No cattle positive for EHEC O157 showed any clinical signs except 2 calves with diarrhea in a veterinary hospital. Almost all isolates possessed the stx gene, and Stx-positive strains carrying both stx 1 and stx 2 genes were predominant. These results indicate that EHEC O157 are distributed in bovine faeces, and that dairy and beef farms in selected regions of Japan are heavily contaminated with the organisms. KEY WORDS: cattle, EHEC O157, prevalence.

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“…E. coli O157:H7 has been implicated in outbreaks from a variety of foods including raw milk, undercooked ground beef, fermented meat, and lettuce (Ackers et al, 1998;Armstrong, Hollingsworth, & Morris, 1996;Hancock, Besser, Rice, Herriott, & Tarr, 1997;Mao, Doyle, & Chen, 2001). Milk contamination occurs usually during milking, although it is possible during storage and transportation.…”

Section: Introductionmentioning

confidence: 99%

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

Gyawali

Adkins

Minor

et al. 2013

CyTA - Journal of Food

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Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine Comportamiento y cambios en la morfología celular de Escherichia coli O157:H7 en un medio líquido y en leche descremada en presencia de cafeína The objective of this study was to investigate the antibacterial effect of caffeine on the behavior and changes in cell morphology of E. coli O157:H7 in a liquid medium and in skim milk. The inhibitory effect of caffeine at different concentrations was determined by inoculating E. coli O157:H7 in laboratory medium and skim milk samples. Samples were incubated at 37ºC for 48 h, and E. coli O157:H7 population was enumerated. Our results showed that caffeine significantly (P < 0.05) inhibited the growth of E. coli O157:H7 in laboratory medium and milk samples. A greater than 3.0 log CFU ml −1 inhibition was observed in milk containing 5.0 g/L caffeine within 12 h of incubation. Moreover, using flow cytometry, marked changes in the morphology of E. coli O157:H7 were also observed. Caffeine has potential as an antimicrobial agent and could be used as an effective natural additive to improve the safety of food products.Keywords: E. coli O157:H7; caffeine; inhibition; milk; flow cytometry El objetivo del presente estudio fue investigar el efecto antibacteriano que tiene la cafeína sobre el comportamiento y los cambios de la morfología celular de E. coli O157:H7 en un medio líquido y en leche descremada. Se determinó el efecto inhibitorio de distintas concentraciones de cafeína, inoculando E. coli O157:H7 en muestras de un medio de laboratorio y de leche descremada. Dichas muestras fueron incubadas a 37°C durante 48 horas, enumerándose posteriormente la población de E. coli O157:H7. Los resultados demostraron que la cafeína inhibió significativamente (P < 0,05) el crecimiento de E. coli O157:H7 tanto en las muestras del medio de laboratorio como en la muestra de leche. En la leche descremada que contenía 5,0 g/L de cafeína, se observó una inhibición mayor a 3,0 log CFU ml -1 , ocurrida dentro de las 12 horas de incubación. Asimismo, a partir del uso de una citometría de flujo se observaron cambios significativos en la morfología de E. coli O157:H7. La cafeína tiene el potencial de ser un agente antimicrobiano, por lo cual podría utilizarse como un aditivo natural efectivo para mejorar la seguridad de los productos alimenticios.Palabras Claves: E. coli O157:H7/; cafeína; inhibición; leche citometría de flujo

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A longitudinal study of Escherichia coli O157 in fourteen cattle herds

Cited by 285 publications

References 13 publications

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

A Three-Year Study of Enterohemorrhagic Escherichia coli O157 on a Farm in Japan

High Prevalence of Enterohemorrhagic Escherichia coli (EHEC) O157 from Cattle in Selected Regions of Japan

Behavior and changes in cell morphology ofEscherichia coliO157:H7 in liquid medium and skim milk in the presence of caffeine

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