A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden

Börjesson, Stefan; Greko, Christina; Myrenås, Mattias; Landén, Annica; Nilsson, Oskar; Pedersen, Karl

doi:10.1016/j.jgar.2019.08.007

Cited by 51 publications

(52 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study demonstrates that broad-spectrum cephalosporin-resistant members of the genus Klebsiella are present in the Austrian horse population, although the prevalence in clinical samples seems to be low. These findings are in accordance with previous studies describing the presence of these particular bacteria in horse populations of other countries [4,[6][7][8][9][10]. Moreover, a previous study carried out in 2018 on clinical samples from Austrian patients reported 8.4% of K. pneumoniae isolates as resistant to third-generation cephalosporins [23].…”

Section: Discussionsupporting

confidence: 92%

“…Wild type wild type parC (Ser80-Ile) parC (Ser80-Ile) 1 ST-sequence type obtained after MLST; 2 n.a.-not applicable; 3 core genome multi-locus sequence-based type; 4 PPP-Posterior probability scores; 5 P-phenotypic resistance: CTX-cefotaxime, CAZ-ceftazidime, FOX-cefoxitin, ATM-aztreonam, GEN-gentamicin, TOB-tobramycin, TET-tetracycline, DOX-doxycycline, CHL-chloramphenicol, SXT-trimethoprim/sulfamethoxazole, CIP-ciprofloxacin; 6 G-genotypic resistance; 7 bold and underline-detected on transconjugats/transformants; 8 bold-within class 1 integron; 9 mutation in gyrA and parC of quinolone resistance-determining region (QRDR). All four K. pneumoniae isolates belonged to different sequence types (ST) and cgMLST complex type (CT) (ST147-CT1202, ST307-CT4645, ST1228-CT4644 and a new ST4848-4643).…”

Section: Qrdrmentioning

confidence: 99%

“…Contrarily, there is still a scarcity of information on broad-spectrum cephalosporin-resistant K. pneumoniae and members of the genus Klebsiella isolated from horses and other domestic animals. To date, only a few equine broad-spectrum cephalosporin-resistant Klebsiella have been isolated and characterized [4,[6][7][8][9][10]. Recent studies reported that some of the characterized resistant K. pneumoniae isolates of equine origin were human-associated multidrug-resistant (MDR) K. pneumoniae [4,8].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria

Loncaric

Rosel

Szostak

et al. 2020

Animals

View full text Add to dashboard Cite

The aim of the present study was to investigate the diversity of broad-spectrum cephalosporin-resistant Klebsiella spp. isolated from horses in Austria that originated from diseased horses. A total of seven non-repetitive cefotaxime-resistant Klebsiella sp. isolates were obtained during diagnostic activities from autumn 2012 to October 2019. Antimicrobial susceptibility testing was performed. The isolates were genotyped by whole-genome sequencing (WGS). Four out of seven Klebsiella isolates were identified as K. pneumoniae, two as K. michiganensis and one as K. oxytoca. All isolates displayed a multi-drug resistant phenotype. The detection of resistance genes reflected well the phenotypic resistance profiles of the respective isolates. All but one isolate displayed the extended-spectrum β-lactamases (ESBL) phenotype and carried CTX-M cefotaximases, whereas one isolate displayed an ESBL and AmpC phenotype and carried cephamycinase (CMY)-2 and sulfhydryl variable (SHV)-type b and Temoniera (TEM) β-lactamases. Among Klebsiella pneumoniae isolates, for different sequence types (ST) could be detected (ST147, ST307, ST1228, and a new ST4848). Besides resistance genes, a variety of virulence genes, including genes coding for yersiniabactin were detected. Considering the high proximity between horses and humans, our results undoubtedly identified a public health issue. This deserves to be also monitored in the years to come.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Qrdrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria

Loncaric

Rosel

Szostak

et al. 2020

Animals

View full text Add to dashboard Cite

show abstract

“…In Latin America, mcr-5 has been found in poultry in Paraguay (9). The description of the mcr-9 homologue from humans in the United States and horses in Sweden has raised attention to another mcr gene with potential for global spread (10). Both mcr variants in this study, 153_Br and 157_Br, showed phenotypic resistance (6) and came from the same farm (Figure, panel B).…”

Section: Research Lettersmentioning

confidence: 63%

mcr-Positive Escherichia coli ST131-H22 from Poultry in Brazil

Saidenberg¹,

Stegger²,

Price³

et al. 2020

Emerg. Infect. Dis.

View full text Add to dashboard Cite

show abstract

“…At SVA, species identification was performed using Bruker MALDI Biotyper System and suspected ESBL/pAmpC-E isolates were thereafter confirmed phenotypically and tested for antibiotic susceptibility using Sensititre ™ EUVSEC and EUVSEC2 microdilution panels (Thermo Fischer Scientific, Waltham, MA USA) while MRCPS was confirmed by polymerase chain reaction [12]. Verified isolates, including the isolate from the 2012 screening, were subjected to next-generation-sequencing using Illumina based technologies and subjected to alignment and bioinformatic analyses as previously described [13]. Serotypes were determined using SerotypeFinder (https ://cge.cbs.dtu.…”

Section: Resultsmentioning

confidence: 99%

Low occurrence of extended-spectrum cephalosporinase producing Enterobacteriaceae and no detection of methicillin-resistant coagulase-positive staphylococci in healthy dogs in Sweden

et al. 2020

Self Cite

View full text Add to dashboard Cite

Sweden has a long tradition of monitoring occurrence of antibiotic resistant bacteria in both animals and humans, but there currently is no organised and harmonized monitoring on carriage of Enterobacteriaceae producing extended-spectrum beta-lactamase (ESBL), plasmid-mediated AmpC beta-lactamase (pAmpC), or methicillin-resistant coagulase positive staphylococci e.g. methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP) in dogs. The aim of the current study was therefore to determine the prevalence of ESBL/pAmpC producing Enterobacteriaceae and methicillin-resistant coagulase positive staphylococci in healthy dogs in Sweden, and to phenotypically and genotypically characterize any identified isolates. It was shown that 0.9% (95% confident interval 0.3-2.7%) of the dogs (n = 325) carried multi-resistant ESBL-producing Escherichia coli, but that no methicillin-resistant coagulase positive staphylococci could be detected. In conclusion, the occurrence of multi-drug resistant bacteria remains rare among healthy dogs in Sweden. In addition, the ESBL-producing E. coli identified showed genetic characteristics related to those reported from humans.

show abstract

A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden

Cited by 51 publications

References 7 publications

Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria

Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria

mcr-Positive Escherichia coli ST131-H22 from Poultry in Brazil

Low occurrence of extended-spectrum cephalosporinase producing Enterobacteriaceae and no detection of methicillin-resistant coagulase-positive staphylococci in healthy dogs in Sweden

Contact Info

Product

Resources

About