mcr-Positive Escherichia coli ST131-H22 from Poultry in Brazil

Saidenberg, André Becker Simões; Stegger, Marc; Price, Lance B.; Johannesen, Thor Bech; Aziz, Maliha; Cunha, Marcos Paulo Vieira; Moreno, Andréa Micke; Knöbl, Terezinha

doi:10.3201/eid2608.191724

Cited by 21 publications

(19 citation statements)

References 8 publications

(18 reference statements)

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“…Whereas E. coli ST131-H30 is the most prevalent sublineage causing extraintestinal infections in humans (Dahbi et al, 2014;Matsumura et al, 2015;Mamani et al, 2019), ST131-H22 predominates in animals, contaminating associated meat products, and can be transmitted zoonotically (Liu et al, 2018;Roer et al, 2019;Saidenberg et al, 2020). Findings from our phylogenetic analysis showed that avian, swine, and human ST131-H22 strains were closely related, supporting results from previous studies (Liu et al, 2018;Reid et al, 2019;Roer et al, 2019;Saidenberg et al, 2020), and also included our environmental strain in the same cluster as those strains (Figure 2), highlighting their transmission at the human-animalenvironment interface.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies have focused on the ST131-H30 sublineage, which is one of the leading causes of extraintestinal infections in humans, including C2 subclade associated with the bla CTX−M−15 gene (Dahbi et al, 2014;Matsumura et al, 2015;Mamani et al, 2019). In contrast, the most prevalent animal ST131 strains belong to the ST131-H22 sublineage and can be transmitted zoonotically, presenting a public health challenge (Liu et al, 2018;Roer et al, 2019;Saidenberg et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

et al. 2021

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The pandemic Escherichia coli sequence type 131 (ST131) carrying plasmid-mediated colistin resistance mcr genes has emerged worldwide causing extraintestinal infections, with lineages belonging to three major clades (A, B, and C). Clade B is the most prevalent in animals, contaminating associated meat products, and can be transmitted zoonotically. However, the blaCTX–M–15 gene has only been associated with C2 subclade so far. In this study, we performed a genomic investigation of an E. coli (strain S802) isolated from a kale crop in Brazil, which exhibited a multidrug-resistant (MDR) profile to clinically significant antimicrobials (i.e., polymyxin, broad-spectrum cephalosporins, aminoglycosides, and fluoroquinolones). Whole-genome sequencing analysis revealed that the S802 strain belonged to serotype O25:H4, ST131/CC131, phylogenetic group B2, and virotype D5. Furthermore, S802 carried the clade B-associated fimH22 allele, genes encoding resistance to clinically important antimicrobials, metals, and biocides, and was phylogenetically related to human, avian, and swine ST131-H22 strains. Additionally, IncHI2-IncQ1, IncF [F2:A-:B1], and ColE1-like plasmids were identified harboring mcr-1.1, blaCTX–M–15, and qnrB19, respectively. The emergence of the E. coli ST131-H22 sublineage carrying mcr-1.1, blaCTX–M–15, and qnrB19 in agricultural soil represents a threat to food and environmental safety. Therefore, a One Health approach to genomic surveillance studies is required to effectively detect and limit the spread of antimicrobial-resistant bacteria and their resistance genes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

et al. 2021

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“…Many antibiotics belonging to different classes, such as tetracyclines (tetracycline, oxytetracycline, chlortetracycline), sulfonamides (sulfadimethoxine, trimethoprim, sulfadiazine, sulfamethazine, sulfaquinoxaline, ormethoprim), aminoglycosides (apramycin, gentamicin, neomycin, spectinomycin), penicillins (amoxicillin, ampicillin), cephalosporins (ceftiofur), quinolones (danofloxacin, sarafloxacin, enrofloxacin), polymyxins (colistin), chloramphenicols (florfenicol), macrolides (erythromycin), and lincosamides (lincomycin) have been used in poultry industry worldwide for the control of APEC infections [ 23 ]. However, APEC resistance to multiple antibiotics has been reported [ 32 , 34 , 35 , 51 , 53 , 54 , 70 , 71 , 72 , 73 , 107 , 108 , 108 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 ], which limits the use of these antibiotics and suggests a challenge ahead in using these antibiotics. Table 2 provides a summary of antibiotic resistance and resistance genes (mechanisms) reported worldwid...…”

Section: Control Strategiesmentioning

confidence: 99%

Avian Pathogenic Escherichia coli (APEC): An Overview of Virulence and Pathogenesis Factors, Zoonotic Potential, and Control Strategies

et al. 2021

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Avian pathogenic Escherichia coli (APEC) causes colibacillosis in avian species, and recent reports have suggested APEC as a potential foodborne zoonotic pathogen. Herein, we discuss the virulence and pathogenesis factors of APEC, review the zoonotic potential, provide the current status of antibiotic resistance and progress in vaccine development, and summarize the alternative control measures being investigated. In addition to the known virulence factors, several other factors including quorum sensing system, secretion systems, two-component systems, transcriptional regulators, and genes associated with metabolism also contribute to APEC pathogenesis. The clear understanding of these factors will help in developing new effective treatments. The APEC isolates (particularly belonging to ST95 and ST131 or O1, O2, and O18) have genetic similarities and commonalities in virulence genes with human uropathogenic E. coli (UPEC) and neonatal meningitis E. coli (NMEC) and abilities to cause urinary tract infections and meningitis in humans. Therefore, the zoonotic potential of APEC cannot be undervalued. APEC resistance to almost all classes of antibiotics, including carbapenems, has been already reported. There is a need for an effective APEC vaccine that can provide protection against diverse APEC serotypes. Alternative therapies, especially the virulence inhibitors, can provide a novel solution with less likelihood of developing resistance.

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“…Investigations of the ExPEC within poultry were mainly focused on the avian pathogenic Escherichia coli (APEC), a subset of ExPEC, from poultry with colibacillosis [ 15 , 16 ] and APEC mainly caused respiratory and systemic disease in poultry; however, the molecular definition criteria of APEC in those studies was different from that of ExPEC [ 6 , 17 ]. Recently, ExPEC isolates within diseased chickens were also reported [ 18 , 19 ]. Notably, the feces of healthy chickens also carried ExPEC isolates [ 14 , 20 ], and the fecal ExPEC isolates could contaminate chicken carcasses at slaughter, including from rupture of the digestive system during processing, and then transmitted to humans by the food chain or direct human-animal contact [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…The presence of antibiotic resistance, one of the ten threats to global health for 2019 as determined by the World Health Organization, among ExPEC isolates has been another big concern. Antibiotic resistance genes, such as extended-spectrum β-lactamases (ESBLs)-encoding genes have been reported in ExPEC isolates [ 22 ] and ExPEC including those from poultry can also acquire different resistance genes [ 18 ], which would inevitably reduce the therapeutic options, increase morbidity and mortality of ExPEC infections, and eventually bring an increased risk to public health [ 23 , 24 ]. Although antibiotics do not select virulent strains such as ExPEC intrinsically [ 25 ], the heavy use of antibiotics in food-producing animals could facilitate the dissemination of ExPEC because such pathogens from environment and diseased animals have been often reported to be multidrug resistant (MDR) [ 7 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiotic Resistance Characteristics of Extraintestinal Pathogenic Escherichia coli among Healthy Chickens from Farms and Live Poultry Markets in China

Zou

Liu

et al. 2021

Animals

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Chicken products and chickens with colibacillosis are often reported to be a suspected source of extraintestinal pathogenic Escherichia coli (ExPEC) causing several diseases in humans. Such pathogens in healthy chickens can also contaminate chicken carcasses at the slaughter and then are transmitted to humans via food supply; however, reports about the ExPEC in healthy chickens are still rare. In this study, we determined the prevalence and characteristics of ExPEC isolates in healthy chickens in China. A total of 926 E. coli isolates from seven layer farms (371 isolates), one white-feather broiler farm (78 isolates) and 17 live poultry markets (477 isolates from yellow-feather broilers) in 10 cities in China, were isolated and analyzed for antibiotic resistance phenotypes and genotypes. The molecular detection of ExPEC among these healthy chicken E. coli isolates was performed by PCRs, and the serogroups and antibiotic resistance characteristics of ExPEC were also analyzed. Pulsed-field gel electrophoresis (PFGE) and Multilocus sequence typing (MLST) were used to analyze the genetic relatedness of these ExPEC isolates. We found that the resistance rate for each of the 15 antimicrobials tested among E. coli from white-feather broilers was significantly higher than that from brown-egg layers and that from yellow-feather broilers in live poultry markets (p < 0.05). A total of 22 of the 926 E. coli isolates (2.4%) from healthy chickens were qualified as ExPEC, and the detection rate (7.7%, 6/78) of ExPEC among white-feather broilers was significantly higher than that (1.6%, 6/371) from brown-egg layers and that (2.1%, 10/477) from yellow-feather broilers (p < 0.05). PFGE and MLST analysis indicated that clonal dissemination of these ExPEC isolates was unlikely. Serogroup O78 was the most predominant type among the six serogroups identified in this study, and all the six serogroups had been frequently reported in human ExPEC isolates in many countries. All the 22 ExPEC isolates were multidrug-resistant (MDR) and the resistance rates to ampicillin (100%) and sulfamethoxazole-trimethoprim (100%) were the highest, followed by tetracycline (95.5%) and doxycycline (90.9%). blaCTX-M was found in 15 of the 22 ExPEC isolates including 10 harboring additional fosfomycin resistance gene fosA3. Notably, plasmid-borne colistin resistance gene mcr-1 was identified in six ExPEC isolates in this study. Worryingly, two ExPEC isolates were found to carry both mcr-1 and blaNDM, compromising both the efficacies of carbapenems and colistin. The presence of ExPEC isolates in healthy chickens, especially those carrying mcr-1 and/or blaNDM, is alarming and will pose a threat to the health of consumers. To our knowledge, this is the first report of mcr-1-positive ExPEC isolates harboring blaNDM from healthy chickens.

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mcr-Positive Escherichia coli ST131-H22 from Poultry in Brazil

Abstract: Her primary research interests are severe respiratory viral infections in children and the elderly, and molecular epidemiology studies.

Cited by 21 publications

References 8 publications

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

Colistin-Resistant mcr-1-Positive Escherichia coli ST131-H22 Carrying blaCTX–M–15 and qnrB19 in Agricultural Soil

Avian Pathogenic Escherichia coli (APEC): An Overview of Virulence and Pathogenesis Factors, Zoonotic Potential, and Control Strategies

Prevalence and Antibiotic Resistance Characteristics of Extraintestinal Pathogenic Escherichia coli among Healthy Chickens from Farms and Live Poultry Markets in China

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