A linear plasmid temperature-sensitive for replication in Streptomyces hygroscopicus 10-22

Abstract

Streptomyces hygroscopicus 10-22 harbors a conjugative, autonomously replicating linear plasmid pHZ6 of ca. 70 kb, which shows no obvious homology with chromosomal DNA and is temperature-sensitive for replication, being stable in the host at 28³C but easily lost at 37³C. On a lawn of the wild-type S. hygroscopicus 10-22 cured of pHZ6, pHZ6 elicit pocks. Temperature sensitivity seemed to be a unique property for pHZ6 among six linear plasmids tested, including the well-known linear plasmids SLP2 in Streptomyces lividans 1326 and SCP1 in Streptomyces coelicolor A3(2). The distinct identity of pHZ6 from previously identified pHZ1^pHZ5 was demonstrated by the profile of relevant plasmids in six well-defined strains originated from S. hygroscopicus 10-22. ß

Antibiotic biosynthetic pathways and pathway engineering—a growing research field in China

“…Inactivation of sanV resulted in the loss of nikkomycin X 15 and Z 16 but the accumulation of nikkomycin Ox and Oz without the methyl group at C3 of HPHT, unambiguously demonstrating the involvment of SanV in HPHT formation. 63 In vitro characterization of SanU and SanV not only revealed the common coenzyme B12 binding feature of glutamate mutases, but also proved their ability of converting glutamate to 3-methylaspartate (23), another immediate precursor for HPHT biosynthesis (Fig. 15).…”

“…40027 demonstrated the use of the two systems. 23 On a lawn of the wildtype S. hygroscopicus 10-22 cured of pHZ6, pHZ6 elicits pocks. Temperature-sensitivity seemed to be a unique property for pHZ6 among six linear plasmids tested, including the well-known linear plasmid SLP2 in S. lividans 1326 and SCP1 in Streptomyces coelicolor A3 (2).…”

Candicidin Biosynthesis Gene Cluster Is Widely Distributed among Streptomyces spp. Isolated from the Sediments and the Neuston Layer of the Trondheim Fjord, Norway

“…In order to demonstrate transfer of the can plasmid, a "curing" experiment with the can plasmid strain was performed in order to obtain a suitable recipient for mating. Loss of linear plasmids as a result of growth at a higher temperature has been demonstrated for Streptomyces (30). A "curing" experiment with Streptomyces sp.…”

Influence of Geosmin-Producing Streptomyces on the Growth and Volatile Metabolites of Yeasts during Chinese Liquor Fermentation

“…19 The plasmid containing the genes can be conjugatively transferred to other streptomycetes. 19 Linear plasmids have also been identified as temperature-sensitive and easily lost, 25 and are commonly transferred to other species. 26 Although the can plasmid is not transferable to other Streptomyces strains in the laboratory, it may be responsible for spreading the candicidin biosynthesis gene cluster to other strains in natural environments such as Chinese liquor-making plants.…”