Antibiotic biosynthetic pathways and pathway engineering—a growing research field in China

Abstract

This review describes the recent research activities in China in relation to studies on antibiotic biosynthetic pathways and pathway engineering in actinomycetes. 75 references are cited.

Recombinational cloning of the antibiotic biosynthetic gene clusters in linear plasmid SCP1 ofStreptomyces coelicolorA3(2)

“…This strategy was based on (1) large antibiotic biosynthetic gene clusters were often cloned and identified in a set of overlapping cosmids, with various sizes of homologous sequences at the ends of the inserts (e.g. Deng & Bai, ); (2) introduction of a circular plasmid containing telomeres into S. lividans (and S. coelicolor ) resulted in the formation of linear replicons (i.e. precise deletion of an E. coli portion bracketing the two telomeres of plasmids) in some clones and circular molecules in others (Qin & Cohen, ; Zhang et al ., ).…”

“…20 to 140 kb; e.g. Deng & Bai, ), we designed here a recombinational procedure. Using this new method, we cloned the 81‐kb avermectin (Ikeda et al ., ) and the 76‐kb spinosad biosynthetic gene clusters (Waldron et al ., ) in SCP1 in S. coelicolor A3(2).…”

DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites

“…pHZ868, a pHZ132 (18,19) derivative carrying the complete dnd gene cluster (Table 1), was digested with XhoI to generate a 1971-bp DNA fragment carrying part of dnd B encoding the C-terminal amino acids, which was recovered from an agarose gel and ligated into the SalI site of pOJ260, giving rise to pJTU155. Oligonucleotide primers dndB-L (5′-CA GAATTC GAAACTTCCCATCACTC-3′, EcoRI site underlined) and dndB-R (5′-CAT GGATCC CTTGTTCAAGATCCG-3′, BamHI site underlined) were used to amplify a 1.85-kb DNA region with an internal 1674-bp EcoRI–BamHI fragment carrying part of the dnd B gene encoding the N-terminal amino acids, using pHZ868 as template.…”

Studies on the dynamic accumulations of Sophora alopecuroides L. Alkaloids in different harvest times and the appropriate harvest time

“…The reference standard of M1-M5 quinolizidine alkaloids was isolated previously from the total alkaloids of S. alopecuroides L. by author, structures of which were elucidated by comparison of spectral data (UV, IR, MS, 1 H NMR and 13 C NMR) with the literature data [11][12][13]. The purity of each reference standard was determined to be above 98% by LC analysis based on a peak area normalisation method, detected by HPLC-PDA and confirmed by LC-ESI-TOF-MS and NMR spectroscopy.…”