“…In this way, the comparisons between different materials, based on their birefringence, will give more accurate and reliable results. The measurement and study of birefringence is not only useful for mineral recognition in petrographical thin sections, but it is also used in characterizing technological materials, such as liquid crystals (Li, X. et al, 2016), optical fibers (Xu, S., et al 2017), in the synthesis of metasurfaces (Achouri, K. et al, 2016), in stiffness calculations of birefringent materials (Neves, N. M., et al, 2004), in identifying the properties of collagen (Wolman, M. & Kasten, F. H., 1986), in the structural characterization of living cells (Mehta, S. B., et al, 2013), and for thickness calculation of calcite crystals in coccolith samples (Beaufort, L., et al, 2014;Gonzalez-Lemos, S. et al, 2017). The wide use of these measurements ensure multiple applications of our calibration methodology for precision characterization and comparisons, especially with a low-cost system.…”