A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis

Guo, Xudong; Xu, Yanxin; Wang, Zikang; Wu, Yukang; Chen, Jiayu; Wang, Guiying; Lu, Chenqi; Jia, Wenwen; Xi, Jiajie; Zhu, Shifeng; Jiapaer, Zeyidan; Wan, Xiaoping; Liu, Zhongmin; Gao, Shaorong

doi:10.1016/j.stem.2018.04.013

Cited by 86 publications

(95 citation statements)

References 38 publications

(52 reference statements)

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“…Eomes, a transcription factor participating in the control of developmental progression, was recruited to Mesp1 promotor by Linc1405. Meanwhile, it has been shown that the Linc1405/Eomes complex united WDR5 and GCN5 to activate Mesp1 in primitive streak, thereby promoting cardiac mesoderm specification and cardiogenesis [107].…”

Section: Lncrnas In Cardiac Developmentmentioning

confidence: 99%

Expedition to the missing link: Long noncoding RNAs in cardiovascular diseases

Yeh

Chang

et al. 2020

J Biomed Sci

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With the advances in deep sequencing-based transcriptome profiling technology, it is now known that human genome is transcribed more pervasively than previously thought. Up to 90% of the human DNA is transcribed, and a large proportion of the human genome is transcribed as long noncoding RNAs (lncRNAs), a heterogenous group of non-coding transcripts longer than 200 nucleotides. Emerging evidence suggests that lncRNAs are functional and contribute to the complex regulatory networks involved in cardiovascular development and diseases. In this article, we will review recent evidence on the roles of lncRNAs in the biological processes of cardiovascular development and disorders. The potential applications of lncRNAs as biomarkers and targets for therapeutics are also discussed.

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Section: Lncrnas In Cardiac Developmentmentioning

confidence: 99%

Expedition to the missing link: Long noncoding RNAs in cardiovascular diseases

Yeh

Chang

et al. 2020

J Biomed Sci

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“…5l, S7b). These genes included early mesoderm transcriptional factors T [31][32][33][34][35][36][37], cardiac transcription factors GATA4, ISL1, TBX3, DES, NKX2.5, TBX5 and TBX18, and sarcomeric genes CTNT, MYH7 (Figs. 5m-n).…”

Section: Atac-seq Reveals Global Alterations Of Chromatin Remodeling mentioning

confidence: 99%

Genome-wide Studies Reveal the Essential and Opposite Roles of ARID1A in Controlling Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells

Liu

Gao

et al. 2020

Preprint

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Background: Early human heart and brain development simultaneously occur during embryogenesis. Notably, in human newborns, congenital heart defects strongly associate with neurodevelopmental abnormalities, suggesting a common gene/complex underlying both cardiogenesis and neurogenesis. However, due to lack of in vivo studies, the molecular 2 mechanisms that govern both early human heart and brain development remain elusive.Results: Here, we report ARID1A, which is a DNA-binding-subunit of the SWI/SNF epigenetic complex, controls both neurogenesis and cardiogenesis from human embryonic stem cells (hESCs) via employing distinct mechanisms. Knockout of ARID1A (ARID1A -/-) led to spontaneous differentiation of neural cells together with globally enhanced expression of neurogenic genes in undifferentiated hESCs. Additionally, when compared with WT hESCs, cardiac differentiation from ARID1A -/-hESCs was prominently suppressed, whereas neural differentiation was significantly promoted. Whole genome-wide scRNA-seq, ATAC-seq, andChIP-seq analyses revealed that ARID1A was required to open chromatin accessibility on promoters of essential cardiogenic genes, and temporally associated with key cardiogenic transcriptional factors T and MEF2C during early cardiac development. However, during early neural development, transcription of most essential neurogenic genes was dependent on ARID1A, which could interact with a known neural restrictive silencer factor REST/NRSF. Conclusions:We uncovered the opposite roles by ARID1A to govern both early cardiac and neural development from pluripotent stem cells. Global chromatin accessibility on cardiogenic genes is dependent on ARID1A, whereas transcriptional activity of neurogenic genes is under control by ARID1A, possibly through ARID1A-REST/NRSF interaction. JL and LY initiated and designed studies. JL performed most experiments. LH performed western blot. XZ and YW worked on ATAC-seq experiments, YS and W.S provided guidance for CRISPR/Cas9 technology, YL provided guidance about scRNA-seq. HG analyzed scRNA-seq data. SL and JW performed bioinformatics analysis on ChIP-seq and ATAC-seq, and did integrative analysis to include other published datasets. JL, JW and LY interpreted data/results and wrote the manuscript. LY supervised the whole research and provided guidance. All authors

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“…During PSC differentiation, Tbx6 promoted mesoderm and endoderm induction in the absence of serum and growth factors in both a cellautonomous and non-cell-autonomous manner (Sadahiro et al 2018). Further studies in mouse will be required to determine how the precise domain of Mesp1 expression is defined within the nascent mesoderm, although other factors are also implicated in early specification of cardiac progenitors, such as long non-coding RNAs (Klattenhoff et al 2013;Ounzain et al 2015;Guo et al 2018), which have even been used to promote trans-differentiation to cardiac cell types (Hou et al 2017) (Figure 3).…”

Section: Mesp1mentioning

confidence: 99%

Cardiopharyngeal Progenitor Specification: Multiple Roads to the Heart and Head Muscles

Swedlund

Lescroart

2019

Cold Spring Harb Perspect Biol

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During embryonic development, the heart arise from various sources of undifferentiated mesodermal progenitors, with an additional contribution from ectodermal neural crest cells. Mesodermal cardiac progenitors are plastic and multipotent, but are nevertheless specified to a precise heart region and cell type very early during development. Recent findings have defined both this lineage plasticity and early commitment of cardiac progenitors, using a combination of single-cell and population analyses. In this review, we discuss several aspects of cardiac progenitor specification. We discuss their markers, fate potential in vitro and in vivo, early segregation and commitment, and also intrinsic and extrinsic cues regulating lineage restriction from multipotency to a specific cell type of the heart. Finally, we also discuss the subdivisions of the cardiopharyngeal field, and the shared origins of the heart with other mesodermal derivatives, including head and neck muscles.

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A Linc1405/Eomes Complex Promotes Cardiac Mesoderm Specification and Cardiogenesis

Cited by 86 publications

References 38 publications

Expedition to the missing link: Long noncoding RNAs in cardiovascular diseases

Expedition to the missing link: Long noncoding RNAs in cardiovascular diseases

Genome-wide Studies Reveal the Essential and Opposite Roles of ARID1A in Controlling Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells

Cardiopharyngeal Progenitor Specification: Multiple Roads to the Heart and Head Muscles

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