A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells

Ding, Lin; Hui, Jingjing; Ju, Huangxian

doi:10.1039/c6cc07574f

Cited by 11 publications

(5 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are four mammalian sialidases, NEU1-4. Lysosomal sialidase NEU1 initiates the degradation of sialoglycoconjugates [16][17][18]; cytosolic sialidase NEU2 exhibits highest activity with gangliosides [19,20]; the plasma membrane-associated sialidase NEU3 is specific for gangliosides [21]; and sialidase NEU4, which is bound to the outer mitochondrial membranes via protein-protein interactions or occurs in the lysosomal lumen, has a wide substrate specificity from glycoproteins to gangliosides and oligosaccharides [22]. The released sialic acids are pumped back into the cytosol, where they can enter another cycle of sialyl glycoconjugate production or be broken down by N-acetylneuraminate lyase (NAL) to ManNAc and pyruvate [23].…”

mentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

120

View full text Add to dashboard Cite

Sialic acids, a subset of nine carbon acidic sugars, often exist as the terminal sugars of glycans on either glycoproteins or glycolipids on the cell surface. Sialic acids play important roles in many physiological and pathological processes via carbohydrate-protein interactions, including cell–cell communication, bacterial and viral infections. In particular, hypersialylation in tumors, as well as their roles in tumor growth and metastasis, have been widely described. Recent studies have indicated that the aberrant sialylation is a vital way for tumor cells to escape immune surveillance and keep malignance. In this article, we outline the present state of knowledge on the metabolic pathway of human sialic acids, the function of hypersialylation in tumors, as well as the recent labeling and analytical techniques for sialic acids. It is expected to offer a brief introduction of sialic acid metabolism and provide advanced analytical strategies in sialic acid studies.

show abstract

mentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

120

View full text Add to dashboard Cite

show abstract

“…The released Neu5Ac enters into another cycle of sialoglycoconjugates in the cytosol. The main distributions of neuraminidases are the cytosol [ 29 ], lysosomal [ 30 ], mitochondria [ 31 ], plasma membrane [ 32 ], etc. Due to the lack of the specific transporter on the cell membrane, extracellular SA enters into the cell by endocytosis or pinocytosis [ 33 ].…”

Section: Main Structures Distributions and Metabolism Of Samentioning

confidence: 99%

“…The released Neu5Ac enters into another cycle of sialoglycoconjugates in the cytosol. The main distributions of neuraminidases are the cytosol [29], lysosomal [30], Meanwhile, the sialoglycoconjugates can be hydrolyzed by neuraminidase (NEU) to form Neu5Ac. The released Neu5Ac enters into another cycle of sialoglycoconjugates in the cytosol.…”

Section: Metabolism Of Samentioning

confidence: 99%

Insights into the Structure, Metabolism, Biological Functions and Molecular Mechanisms of Sialic Acid: A Review

Li,

Lin,

Luo

et al. 2023

Foods

View full text Add to dashboard Cite

Sialic acid (SA) is a kind of functional monosaccharide which exists widely in edible bird’s nest (EBN), milk, meat, mucous membrane surface, etc. SA is an important functional component in promoting brain development, anti-oxidation, anti-inflammation, anti-virus, anti-tumor and immune regulation. The intestinal mucosa covers the microbial community that has a significant impact on health. In the gut, SA can also regulate gut microbiota and metabolites, participating in different biological functions. The structure, source and physiological functions of SA were reviewed in this paper. The biological functions of SA through regulating key signaling pathways and target genes were discussed. In summary, SA can modulate gut microbiota and metabolites, which affect gene expressions and exert its biological activities. It is helpful to provide scientific reference for the further investigation of SA in the functional foods.

show abstract

“…This strategy could be applied to monitor the ST activity during drug treatment and discriminate tumor and normal cells. To detect intracellular Neus, they delivered a lysosomal Neu-specific substrate 4-methylumbelliferyl- N -acetylneuraminic acid into lysosomes, which could release the cleavage product of 4-methylumbelliferone into the cytosol for neutral pH-enhanced fluorescence detection of lysosomal Neu activity in living cells . The designed strategy enabled the monitor of the upregulation of lysosomal Neu induced by sodium butyrate, and the dynamic tracking of the variation of lysosomal Neu activity in living cells during the apoptosis process and drug treatment.…”

Section: Intracellular Glycans Analysismentioning

confidence: 99%

In Situ Cellular Glycan Analysis

Chen

Ding

2018

Acc. Chem. Res.

Self Cite

View full text Add to dashboard Cite

Glycan decorates all mammalian cell surfaces through glycosylation, which is one of the most important post-modifications of proteins. Glycans mediate a wide variety of biological processes, including cell growth and differentiation, cell-cell communication, immune response, pathogen interaction, and intracellular signaling events. Besides, tumor cells aberrantly express distinct sets of glycans, which can indicate different tumor onsets and progression processes. Thus, analysis of cellular glycans may contribute to understanding of glycan-related biological processes and correlation of glycan patterns with disease states for clinical diagnosis and treatment. Although proteomics and glycomics have included great efforts for in vitro study of glycan structures and functions using lysis samples of cells or tissues, they cannot offer real-time qualitative or quantitative information, especially spatial distribution, of glycans on/in intact cells, which is important to the revelation of glycan-related biological events. Moreover, the complex lysis and separation procedures may bring unpredictable loss of glycan information. Focusing on the great urgency for in situ analysis of cellular glycans, our group developed a series of methods for in situ analysis of cellular glycans in the past 10 years. By construction of electrochemical glycan-recognizable probes, glycans on the cell surface can be quantified by direct or competitive electrochemical detection. Using multichannel electrodes or encoded lectin probes, multiple glycans on the cell surface can be dynamically monitored simultaneously. Through design of functional nanoprobes, the cell surface protein-specific glycans and intracellular glycan-related enzymes can be visualized by fluorescence or Raman imaging. Besides, some biological enzymes-based methods have been developed for remodeling or imaging of protein-specific glycans and other types of glycoconjugates, such as gangliosides. Through tracing the changes of glycan expression induced by drugs or gene interference, some glycan-related biological processes have been deduced or proved, demonstrating the reliability and practicability of the developed methods. This Account surveys the key technologies developed in this area, along with the discussion on the shortages of current methodology as well as the possible strategies to overcome those shortages. The future trend in this topic is also discussed. It is expected that this Account can provide a versatile arsenal for chemical and biological researchers to unravel the complex mechanisms involved in glycan-related biological processes and light new beacons in tumor diagnosis and treatment.

show abstract

A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in living cells

Cited by 11 publications

References 24 publications

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Insights into the Structure, Metabolism, Biological Functions and Molecular Mechanisms of Sialic Acid: A Review

In Situ Cellular Glycan Analysis

Contact Info

Product

Resources

About